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12,913 results

Showing: 3201 - 3250 of 12913 results

  2. pcDNA3.1-nAChr-Alpha6-GFP

    Plasmid
    #50486
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Tags
    GFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the GFP open…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. pSCALPs-HA-NFAT1 (4-460)-GFP

    Plasmid
    #88879
    Purpose
    Lentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFP
    Depositor
    Silvia Monticelli
    Article
    Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi
    Insert
    HA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)
    Use
    Lentiviral
    Tags
    GFP fusion protein and HA tag
    Expression
    Mammalian
    Mutation
    amino acids 4-460 only; L78P & L287P *see bel…
    Promoter
    SFFV (spleen focus forming virus)
    Available Since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    88879-plasmid-map-sequence-id-177202

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  5. MSCV-FoxP3-IRES-Thy1.1

    Plasmid
    #229616
    Purpose
    Mammalian expression of full length mouse FoxP3
    Depositor
    Sun Hur
    Insert
    full length FoxP3 (Foxp3 Mouse)
    Expression
    Mammalian
    Mutation
    none
    Available Since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229616-plasmid-map-sequence-id-457336

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  6. MSCV-FoxP3-R337Q-IRES-Thy1.1

    Plasmid
    #229617
    Purpose
    Mammalian expression of full length mouse FoxP3 (R337Q)
    Depositor
    Sun Hur
    Insert
    full length FoxP3, carrying single point mutation R337Q (Foxp3 Mouse)
    Expression
    Mammalian
    Mutation
    R337Q
    Available Since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229617-plasmid-map-sequence-id-457335

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  7. MSCV-FoxP3-A372S-IRES-Thy1.1

    Plasmid
    #229618
    Purpose
    Mammalian expression of full length mouse FoxP3 (A372S)
    Depositor
    Sun Hur
    Insert
    full length FoxP3, carrying single point mutation A372S (Foxp3 Mouse)
    Expression
    Mammalian
    Mutation
    A372S
    Available Since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229618-plasmid-map-sequence-id-457333

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  8. MSCV-FoxP3-R337Q/A372S-IRES-Thy1.1

    Plasmid
    #229619
    Purpose
    Mammalian expression of full length mouse FoxP3 (R337Q, A372S)
    Depositor
    Sun Hur
    Insert
    full length FoxP3, carrying double mutation R337Q/A372S (Foxp3 Mouse)
    Expression
    Mammalian
    Mutation
    R337Q/A372S
    Available Since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229619-plasmid-map-sequence-id-457331

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  11. pENTR11 CA NFAT2

    Plasmid
    #11793
    Depositor
    Anjana Rao
    Insert
    NFAT2 (Nfatc1 Mouse)
    Expression
    Bacterial
    Mutation
    Constitutively active: multiple serine to alanine…
    Available Since
    Aug. 7, 2006
    Availability
    Academic Institutions and Nonprofits only

  16. pLV-Enh eGFP Reporter-muKC2-X1

    Plasmid
    #59200
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    krt1-ex1
    Use
    Lentiviral
    Promoter
    Mu Hsp68 minimal promoter
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. Per2:iLuc

    Plasmid
    #160293
    Purpose
    target Per2 gene with a color-switching luciferase cassette in mouse genome and the transfection of the recombinant DNA will lead genomic recombination and make a knock-in allele in mouse ES cells.
    Depositor
    Joseph Takahashi
    Article
    Shan et al Neuron. 2020 Aug 4. pii: S0896-6273(20)3
    Insert
    Per2LUC
    Expression
    Mammalian
    Promoter
    no promotor
    Available Since
    Dec. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    160293-plasmid-map-sequence-id-318636

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  19. pAAV-shMcu-2

    Plasmid
    #181867
    Purpose
    Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter
    Depositor
    Hilmar Bading
    Article
    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc
    Insert
    mitochondrial calcium uniporter (Mcu Mouse)
    Use
    AAV, Adenoviral, and Mouse Targeting
    Tags
    hrGFP
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181867-plasmid-map-sequence-id-356033

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  29. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2

    Plasmid
    #99694
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Actc1 (gRNA: GGTATATAAGGGGTTTTAAG) (Actc1 Synthetic, Mouse)
    Use
    AAV and CRISPR
    Tags
    VPR mini
    Mutation
    dead Cas9
    Available Since
    Jan. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    99694-plasmid-map-sequence-id-194587

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  30. Dronpa-H3.3-N-14

    Plasmid
    #57278
    Purpose
    Localization: Nucleus/Histones, Excitation: 503, Emission: 518
    Depositor
    Michael Davidson
    Insert
    H3.3 (H3f3b Mouse)
    Tags
    Dronpa
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. pcDNA3.1-m-Flt4

    Plasmid
    #119232
    Purpose
    Plasmid encodes full-length murine VEGFR-3 gene cloned in mammalian expression vector.
    Depositor
    Kari Alitalo
    Insert
    Vegfr3 (Flt4 Mouse)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    119232-plasmid-map-sequence-id-231668

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  37. pcDNA-LBR-EGFP-polyA

    Plasmid
    #59748
    Purpose
    Express LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.
    Depositor
    Yi Zhang
    Article
    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-
    Insert
    LBR-EGFP
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    Sept. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    59748-plasmid-map-sequence-id-94052

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  39. pBS-AMVpl-δWT-nAchR

    Plasmid
    #97188
    Purpose
    To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co
    Depositor
    Henry Lester
    Article
    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.
    Insert
    Delta nicotinic acetylcholine receptor (Chrnd Mouse)
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    T7
    Available Since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    97188-plasmid-map-sequence-id-191155

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  44. pEF1-His-Ub

    Plasmid
    #159326
    Purpose
    Mammalian cell expression of mouse His-Ub
    Depositor
    Jianjun Zhao
    Insert
    His-Ub
    Expression
    Mammalian
    Available Since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    159326-plasmid-map-sequence-id-311850

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  49. pCX-EGFP c-Abl

    Plasmid
    #15007
    Depositor
    Raymond Birge
    Insert
    c-Abl (Abl1 Mouse)
    Tags
    IRES EGFP
    Expression
    Mammalian
    Available Since
    Aug. 22, 2007
    Availability
    Academic Institutions and Nonprofits only
Showing: 3201 - 3250 of 12913 results