We narrowed to 7,680 results for: CCH

Showing: 3251 - 3300 of 7680 results

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LD105

Plasmid

#69162

Purpose

Integration of GFP tag at Atg5 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg5-EGFP

Tags

EGFP

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCyc-Bem1

Plasmid

#66573

Purpose

Express an additional copy of Bem1, integrated at TRP locus

Depositor

Orion Weiner

Article

Jost et al ACS Synth Biol. 2015 Jun 2.

Insert

Bem1

Expression

Yeast

Mutation

F99I*(see below)

Promoter

pCyc1

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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LD131

Plasmid

#69177

Purpose

Integration of GFP tag at Atg31 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg31-EGFP

Tags

EGFP

Expression

Yeast

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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LD129

Plasmid

#69176

Purpose

Integration of GFP tag at Atg29 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg29-EGFP

Tags

EGFP

Expression

Yeast

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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LD124

Plasmid

#69174

Purpose

Integration of GFP tag at Atg24 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg24-EGFP

Tags

EGFP

Expression

Yeast

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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LD120

Plasmid

#69171

Purpose

Integration of GFP tag at Atg20 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg20-EGFP

Tags

EGFP

Expression

Yeast

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1509

Plasmid

#62363

Purpose

pFA6a BirA-heh2 linker-SSH1::HIS5

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA-heh2-Ssh1

Expression

Yeast

Promoter

Gal1

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pMZ286

Plasmid

#52227

Purpose

Expression of sgRNA precursor targeted against ade6L469

Depositor

Mikel Zaratiegui

Article

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Insert

rrk1-sgRNA

Use

CRISPR

Expression

Yeast

Promoter

rrk1

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pDL96

Plasmid

#43910

Depositor

Stephen Elledge

Article

Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

Insert

DIF1 (DIF1 Budding Yeast)

Expression

Yeast

Mutation

AAEAAA

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pLG-PLint

Plasmid

#33145

Depositor

Michael Rosbash

Article

Legrain et al Cell. 1989 May 19;57(4):573-83.

Insert

RP51A-synthetic minimal intron (RPS17A Budding Yeast)

Tags

LacZ

Expression

Yeast

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pMal672H

Plasmid

#25817

Depositor

Cynthia Wolberger

Article

Feeser et al J Mol Biol. 2008 Jul 11. 380(3):520-31.

Insert

Rap1(672-827) (RAP1 Budding Yeast)

Tags

His6 and MBP

Expression

Bacterial

Available Since

July 26, 2010

Availability

Academic Institutions and Nonprofits only
pGC295

Plasmid

#19665

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*iA-4xNLS-YFP::let-858(3’)

Use

Flp/frt

Tags

4xNLS

Expression

Worm

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pGC292

Plasmid

#19645

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*iA-4xNLS-GFP::let-858(3’)

Use

Flp/frt

Tags

4xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pGC232

Plasmid

#19650

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Use

Flp/frt

Tags

4xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pGC296

Plasmid

#19651

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*iA-4xNLS-tdimer2(12)::let-858(3’)

Use

Flp/frt

Tags

4xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pGC309

Plasmid

#19652

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*iA-1xNLS-tdimer2(12)::let-858(3’)

Use

Flp/frt

Tags

1xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pGC294

Plasmid

#19661

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*iA-4xNLS-CFP::let-858(3’)

Use

Flp/frt

Tags

4xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pGC258

Plasmid

#19664

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Use

Flp/frt

Tags

4xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pGC308

Plasmid

#19666

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*iA-1xNLS-YFP::let-858(3’)

Use

Flp/frt

Tags

1xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pGC382

Plasmid

#19667

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*-YFP::let-858(3’)

Use

Flp/frt

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pYBS369

Plasmid

#18885

Depositor

Elaine Elion

Article

Choi et al Cell. 1994 Aug 12. 78(3):499-512.

Insert

Ste7 (STE7 Budding Yeast)

Expression

Yeast

Mutation

amino terminal 172 aa of Ste7

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
pYBS370

Plasmid

#18886

Depositor

Elaine Elion

Article

Choi et al Cell. 1994 Aug 12. 78(3):499-512.

Insert

Ste7 (STE7 Budding Yeast)

Expression

Yeast

Mutation

kinase domain of C-terminal 344 residues

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
pPS879

Plasmid

#8906

Depositor

Pamela Silver

Article

Lee et al Genes Dev 1996 May 15;10(10):1233-46.

Insert

NPL3 F160L (NPL3 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

F160L

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pPS1523

Plasmid

#8919

Depositor

Pamela Silver

Article

Taura et al Proc Natl Acad Sci U S A 1998 Jun 23;95(

Insert

YRB2 (YRB2 Budding Yeast)

Tags

GFP

Expression

Yeast

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pPS1069

Plasmid

#1354

Depositor

Pamela Silver

Article

Seedorf et al Proc Natl Acad Sci U S A 1997 Aug 5;94(1

Insert

PSE1 (PSE1 Budding Yeast)

Tags

sGFP

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pPS1356

Plasmid

#1379

Depositor

Pamela Silver

Article

Shen et al Genes Dev 1998 Mar 1;12(5):679-91.

Insert

NPL3 (NPL3 Budding Yeast)

Tags

Myc

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pLOX-gfp-iresTK

Plasmid

#12243

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

GFP, HSV1-TK

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

LoxP in 3' LTR.

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pCAG-Flpe:GFP

Plasmid

#13788

Purpose

Mammalian expression of enhanced form of Flp recombinase fused to GFP

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Flpe-GFP fusion protein

Use

Flp/frt

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pCAG-Flpe

Plasmid

#13787

Purpose

Mammalian expression of enhanced form of Flp recombinase with C-terminal Myc tag

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Flpe

Tags

Myc

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
Tubp-Gal80 in pCaSpeR4

Plasmid

#17748

Depositor

Liqun Luo

Article

Lee et al Neuron. 1999 Mar . 22(3):451-61.

Insert

GAL80 (GAL80 Budding Yeast)

Tags

SV40 polyA and Tubulin 1alpha promoter

Expression

Insect

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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pYES2-Ub-G76V-GFP

Plasmid

#11954

Depositor

Nico Dantuma

Article

Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

Insert

Ub-G76V-GFP

Expression

Yeast

Mutation

Ubiquitin fused to N-terminus of GFP. Glycine 76 …

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTy1mhis3-AI or pGTy1his3-AI

Plasmid

#62228

Purpose

A Ty1 element tagged with the retrotransposition indicator gene his3-AI is fused to the GAL1 promoter on a multicopy URA3-based shuttle plasmid.

Depositor

David Garfinkel

Article

Curcio et al Proc Natl Acad Sci U S A. 1991 Feb 1;88(

Insert

Ty1H3 retrotransposon

Expression

Yeast

Promoter

GAL1

Available Since

Feb. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pESC-URA (V5 and HA)

Plasmid

#222485

Purpose

Co-expression of two genes in yeast with V5 and HA tags and URA3 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

HA and V5

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD33-Gm

Plasmid

#65098

Purpose

bacterial expression vector with Gentamycin selection, based on pBAD33

Depositor

Juan Tomas

Article

Jimenez et al J Bacteriol. 2009 Apr;191(7):2228-36. do

Type

Empty backbone

Expression

Bacterial

Promoter

pBAD

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL0_16 [2μ ORI]

Plasmid

#198929

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

2μ ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDDGFP_LEU2D

Plasmid

#58352

Purpose

Used for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiae

Depositor

Simon Newstead

Article

Parker et al Protein Sci. 2014 Jun 20. doi: 10.1002/p

Type

Empty backbone

Tags

GFP-His

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pESC-LEU (V5 and HA)

Plasmid

#222486

Purpose

Co-expression of two genes in yeast with V5 and HA tags and LEU2 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

HA and V5

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-AKAR4

Plasmid

#61619

Purpose

FRET-based biosensor for monitoring PKA activity

Depositor

Jin Zhang

Article

Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

Insert

AKAR4

Tags

Cerulean and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
VT028327-FRT10-STOP-FRT10-GAL4

Plasmid

#232829

Purpose

Enhancer VT028327 driven GAL4, gated by Sparse.FRT10

Depositor

Liqun Luo

Article

Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

Inserts

FRT10-STOP-FRT10
GAL4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV0133

Plasmid

#133467

Purpose

Integration into fission yeast genome at ura4 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Type

Empty backbone

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV0356

Plasmid

#133468

Purpose

Integration into fission yeast genome at ade6 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Type

Empty backbone

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV0413

Plasmid

#133470

Purpose

Integration into fission yeast genome at his5 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Type

Empty backbone

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV0357

Plasmid

#133469

Purpose

Integration into fission yeast genome at lys3 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Type

Empty backbone

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a MDHM_YEAST

Plasmid

#204231

Purpose

Bacterial expression of yeast mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

Depositor

Malate dehydrogenase CUREs Community (MCC)

Insert

MDHM_YEAST (MDH1 Budding Yeast)

Tags

6X His

Expression

Bacterial

Mutation

Does not include transit peptide and includes a M…

Promoter

Lac

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pESC-TRP (V5 and HA)

Plasmid

#222488

Purpose

Co-expression of two genes in yeast with V5 and HA tags and TRP1 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

HA and V5

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Tubp-Gal4 in pCaSpeR4

Plasmid

#17747

Depositor

Liqun Luo

Article

Lee et al Neuron. 1999 Mar . 22(3):451-61.

Insert

GAL4 (GAL4 Budding Yeast)

Tags

Tubulin 1alpha promoter and hsp70 PolyA

Expression

Insect

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pWL078 MCP-2xGFP

Plasmid

#197047

Purpose

pCYC-NLS-MCP-2xGFP on pRS305

Depositor

Robert Singer

Article

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

MCP-2xGFP

Expression

Yeast

Promoter

CYC1

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTYB1-eIF4A

Plasmid

#37225

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

eIF4A

Tags

intein

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pPT24-mtnluc

Plasmid

#184339

Purpose

Transformation of mitochondrial DNA in yeast

Depositor

Martin Ott

Article

Rzepka et al STAR Protoc. 2022 May 25;3(2):101359. do

Insert

nanoluciferase recoded for mitochondrial expression flanked by COX2 UTRs

Expression

Yeast

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBPGAL4.2::VP16Uw

Plasmid

#26228

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

GAL4::VP16 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 3251 - 3300 of 7680 results

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We narrowed to 7,680 results for: CCH