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pBEL1994

Plasmid

#139898

Purpose

Expression plasmid for LetB PLL2 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; delta229-239, D228G, L240G)-6xHis

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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sgDonor_lentiGuide

Plasmid

#184168

Purpose

LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Insert

sgDonor

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry

Plasmid

#108884

Purpose

Multisite gateway vector for 3' tagging with mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mCherry

Use

Multisite gateway

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pET30b-7Y-SrtA

Plasmid

#105601

Purpose

expresses 7Y sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7Y mutant

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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ICP8-P2A/Crimson (pSLIK4)

Plasmid

#113859

Purpose

For Doxycycline-inducible expression of ICP8 synaptase gene, with ICP8-P2A-red fluorescent protein gene E2-Crimson

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

ICP8

Use

Lentiviral

Tags

P2A/Crimson

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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p3E p2a-tomato

Plasmid

#135211

Purpose

Red fluorescent tag

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

tdTomato

Use

Synthetic Biology

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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H2BK120C

Plasmid

#75332

Purpose

Bacteria expression of H2BK120C

Depositor

Cynthia Wolberger

Article

Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

Insert

H2BK120C (h2bc11.S Frog)

Tags

none

Expression

Bacterial

Mutation

truncated; K120C

Promoter

T7

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDL1

Plasmid

#182263

Purpose

Gibson cloning vector for synthesis of single and double stranded RNA

Depositor

Daniel Lobo

Article

Wolff et al STAR Protoc. 2022 Jun 14;3(3):101458. do

Type

Empty backbone

Use

Gibson cloning vector for synthesis of single and…

Promoter

T3 and SP6 for sense and antisense riboprobe synt…

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pUSE-RapR-Src-myc

Plasmid

#25933

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-Src (Src Mouse)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 9, 2010

Availability

Academic Institutions and Nonprofits only

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csr-1_hH1_mEos3.1_V5

Plasmid

#191750

Purpose

Photoconvertible fluorescent nuclear reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

hH1 (hH1 Neurospora crassa)

Tags

V5, ccg-1 promoter, and mEos3.1

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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nuc-gTEMP_pcDNA3

Plasmid

#109118

Purpose

Nucleus targetting gTEMP

Depositor

Takeharu Nagai

Article

Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

Insert

nuc-gTEMP

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pET-AqpZ-ALFA

Plasmid

#212310

Purpose

Expression of AqpZ with ALFA tag

Depositor

Arthur Laganowsky

Article

Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

Insert

Aquaporin Z

Tags

ALFA tag and Strep-Tag II

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-Chimeric_Butterfly_CY_1.0

Plasmid

#59800

Purpose

Genetically encoded fluorescent voltage indicator

Depositor

Thomas Knopfel

Article

Mishina et al Front Mol Neurosci. 2014 Sep 29;7:78. do

Insert

Cyan-Yellow Chimeric Butterfly Voltage Sensitive Fluorescent Protein

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAd5-B6-deltaE3

Plasmid

#122559

Purpose

Modified block 6, with deletion in E3

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 27859-30803

Use

Synthetic Biology

Mutation

Adenovirus sequences 27859-30803 replaced with Ba…

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-RNF169

Plasmid

#119004

Purpose

Mammalian expression of human RNF169 and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

RNF169 (RNF169 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJB153

Plasmid

#92328

Purpose

Express mEos3.2 tagged ZapA in E coli

Depositor

Jie Xiao

Article

Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

Insert

ZapA

Tags

mEos3.2

Expression

Bacterial

Promoter

T5-lac

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pSTARR-seq_fly-hsp70

Plasmid

#71500

Purpose

flySTARR-seq screening vector with hsp70 core promoter

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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1455_pDEST_miniTol2_R4-R2_MCS

Plasmid

#171794

Purpose

pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a Multiple Cloning Site for selection marker insertion.

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Type

Empty backbone

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 VPS50

Plasmid

#175500

Purpose

VPS50-GFP is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Schindler et al Nat Cell Biol. 2015 May;17(5):639-50. do

Insert

Vps50 (Vps50 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAC14

Plasmid

#197408

Purpose

Honey bee gut symbiont broad-host range vector with constitutive GFP, ampR and pTFC-FC2 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

pTF-FC2 replicon

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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