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poly E67R SUMO1

Plasmid

#221072

Purpose

Expression of poly E67R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of E67R SUMO1 each separated by (GGS)4 (SUMO1 Human)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

E67R

Promoter

tac

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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poly H35K SUMO1

Plasmid

#221070

Purpose

Expression of poly H35K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of H35K SUMO1 each separated by (GGS)4 (SUMO1 Human)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

H35K

Promoter

tac

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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poly H35R SUMO1

Plasmid

#221069

Purpose

Expression of poly H35R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of H35R SUMO1 each separated by (GGS)4 (SUMO1 Human)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

H35R

Promoter

tac

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pJCC_162 SpCas9 KES(1107-1109)GG

Plasmid

#179526

Purpose

For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

Insert

SpCas9 KES(1107-1109)GG

Use

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

replaced KES (residues 1107-1109) with GG

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pJCC_163 SpCas9 K233A/K234A/K253A/K263A

Plasmid

#179527

Purpose

For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

Insert

SpCas9 K233A/K234A/K253A/K263A

Use

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed lysines 233, 234, 253, and 263 to alanine

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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EcRfaHKOW-L1L2NusG

Plasmid

#113126

Purpose

Expresses C-terminus of E. coli RfaH with L1 and L2 loops replaced with NusG residues

Depositor

James Berger

Article

Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

Insert

RfaH (rfaH Escherichia coli)

Use

Tags

His6-MBP-N10-TEV

Expression

Bacterial

Mutation

Deleted residues 1-107, replaced L1 and L2 loops …

Promoter

T7

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pMTTH+mEGFP-H1.4ΔCTD

Plasmid

#157800

Purpose

Expression of mEGFP fused to human linker histone H1.4 without c-terminal tail

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

mEGFP-H1.4ΔCTD (H1-4 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

pTAC

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pMTTH+LANA-H1.4CTD

Plasmid

#157798

Purpose

Expression of LANA peptide-human linker histone H1.4 CTD fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

LANA-H1.4CTD (H1-4 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

pTAC

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pHTSHP-ShhN-Cys

Plasmid

#121136

Purpose

Expresses ShhN with a C-terminal cysteine residues in bacteria

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

shh (Shh Mouse)

Use

Tags

HIS, HRV3C site, and MBP

Expression

Bacterial

Mutation

Amino acid sequence from 26-191, with an addition…

Promoter

tac

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral N

Plasmid

#113009

Purpose

For bacterial expression of MBP fusion of N terminal region of Drosophila Tral

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

N terminus of tral (tral Fly)

Use

Tags

Expression

Bacterial

Mutation

amino acids 1-355

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral C

Plasmid

#113008

Purpose

For bacterial expression of MBP fusion of C terminal region of Drosophila Tral

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

C terminus of tral (tral Fly)

Use

Tags

Expression

Bacterial

Mutation

amino acids 356-652

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

Plasmid

#101207

Purpose

Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant N497A/R661A/Q695A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

N497A, R661A and Q695A

Promoter

T7

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant

Plasmid

#101201

Purpose

Bacterial expression plasmid for SpCas9, REC3 FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/S701C/S960C

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, S701C and S960C

Promoter

T7

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

Plasmid

#101214

Purpose

Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/S355C/S867C/K855A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, S355C, S867C and K855A

Promoter

T7

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant

Plasmid

#101200

Purpose

Bacterial expression plasmid for SpCas9, REC2 FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/E60C/D273C

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, E60C and D273C

Promoter

T7

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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hSRAP.HMG6.wt.Dlnk.a13-236

Plasmid

#49814

Purpose

expression vector for MBP-SRAP(13-236) fusion

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein (SRA1 Human)

Use

Tags

E. coli Maltose Binding Protein

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCDF-rM1PYK

Plasmid

#220232

Purpose

Inducible expression of rabbit muscle pyruvate kinase isoform 1

Depositor

Aron Fenton, Liskin Swint-Kruse

Article

Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

Insert

Rabbit muscle pyruvate kinase (PKM )

Use

Tags

6xHis-MBP-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-XPG

Plasmid

#39215

Depositor

Aziz Sancar

Article

Matsunaga et al J Biol Chem. 1995 Sep 1;270(35):20862-9.

Insert

XPG (ERCC5 Human)

Use

Tags

MBP

Expression

Bacterial

Mutation

Note that XPG starts at amino acid 5

Promoter

Ptac

Available since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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pFastBac1-LRRK2 WD40

Plasmid

#139211

Purpose

Human LRRK2 WD40 domain (residues 2142–2527) was expressed in Sf9 insect cells via baculovirus infection and purified to homogeneity.

Depositor

Hao Wu

Article

Zhang et al Proc Natl Acad Sci U S A. 2019 Jan 29;11

Insert

LRRK2 (LRRK2 Human)

Use

Tags

N-His and MBP

Expression

Insect

Mutation

Promoter

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pET 2C-T10-dCasX

Plasmid

#126418

Purpose

E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

Depositor

Jennifer Doudna

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

DpbCasX

Use

CRISPR

Tags

His-MBP

Expression

Bacterial

Mutation

Promoter

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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