We narrowed to 15,050 results for: pET

1. pET-51b(+)-Caprola_01

   Plasmid

   #194662

   Purpose

   Protein production of the split HaloTag based calcium recorder Caprola_01 in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_01
   

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaG-30

   Plasmid

   #172754

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-30
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET24-ISWI-iCBD

   Plasmid

   #105499

   Purpose

   Expression of Drosophila ISWI C-terminally tagged with Combined Sce VMA intein and chitin binding domain (iCBD)

   Depositor

   James Kadonaga

   Article

   Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

   Insert

   ISWI-iCBD
   

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-His-MinD_MinE

   Plasmid

   #133621

   Purpose

   Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.

   Depositor

   Petra Schwille

   Article

   Loose et al Science. 2008 May 9;320(5877):789-92. do

   Insert

   His-MinD_MinE (minD, minE E. coli)
   

   Expression

   Bacterial

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-HaloTag7SS-DogTag

   Plasmid

   #171775

   Purpose

   Expresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formation

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   HaloTag7SS-DogTag
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   DogTag inserted in HaloTag7 between residues D139…

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a-hAIM2-His6

   Plasmid

   #51532

   Purpose

   Express His6-tagged hAIM2 in bacteria

   Depositor

   Emad Alnemri

   Article

   Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

   Insert

   AIM2 (AIM2 Human)
   

   Tags

   His6 and T7

   Expression

   Bacterial

   Promoter

   lac operator

   Available Since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21-pelB-LaM-2

   Plasmid

   #172768

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-2
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. wt 6XHis-SNX9 pET15b

   Plasmid

   #34690

   Depositor

   Sandra Schmid

   Article

   Yarar et al Traffic. 2008 Jan;9(1):133-46. Epub 2007

   Insert

   Sorting nexin 9
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Jan. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pET28a-SUMO-AsCas12f

   Plasmid

   #171612

   Purpose

   AsCas12f1 heterologous expression plasmid

   Depositor

   Quanjiang Ji

   Article

   Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

   Insert

   AsCas12f1
   

   Expression

   Bacterial

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28b-HRASLS3-1-132

    Plasmid

    #100725

    Purpose

    Expression of N-terminal region of human HRASLS3 in E.coli

    Depositor

    Byung Il Lee

    Article

    Han et al Protein Expr Purif. 2010 May;71(1):103-7

    Insert

    HRASLS3 (PLAAT3 Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7 promotor

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-51b(+)-cpHalo∆

    Plasmid

    #194659

    Purpose

    Protein production of the split HaloTag part cpHalo∆ (cpHaloTag135/156) in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    cpHalo∆
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-AqpZ-ALFA

    Plasmid

    #212310

    Purpose

    Expression of AqpZ with ALFA tag

    Depositor

    Arthur Laganowsky

    Article

    Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

    Insert

    Aquaporin Z
    

    Tags

    ALFA tag and Strep-Tag II

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15b-17X-PTDH

    Plasmid

    #166786

    Purpose

    Phosphite dehydrogenase for NAD(P)H regeneration

    Depositor

    Wilfred van der Donk

    Article

    Yang et al J Am Chem Soc. 2015 Oct 7;137(39):12426-

    Insert

    Phosphite dehydroenase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    A196R, T201S, A328T, E352N, and C356D- please see…

    Promoter

    T7

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET22b-His6-UBCH7

    Plasmid

    #212711

    Purpose

    Bacterial expression of His6-UBCH7

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    UBCH7
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-BCA-Q11

    Plasmid

    #185781

    Purpose

    Encodes enzyme bovine carbonic anhydrase linked to Q11 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    BCA-Q11
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28C-mCherry-YTHDF2

    Plasmid

    #204406

    Purpose

    E. coli expression of full-length YTHDF2 with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21a-hAIM2-GST

    Plasmid

    #51528

    Purpose

    Express GST-tagged hAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    AIM2 (AIM2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    lac operator

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLicC-MBP-APETx2

    Plasmid

    #72668

    Purpose

    Periplasmic expression of APETx2 in E.coli

    Depositor

    Glenn King

    Article

    Anangi et al Mar Drugs. 2012 Jul;10(7):1605-18. doi:

    Insert

    APETx2
    

    Tags

    His tag, MBP, MalE signal sequence for periplasmi…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-Mff(1-61)-PP-GST

    Plasmid

    #73042

    Purpose

    Bacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage site

    Depositor

    David Chan

    Article

    Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

    Insert

    Mff(1-61) (Mff Mouse)
    

    Tags

    GST-tag and PreScission protease site

    Expression

    Bacterial

    Mutation

    Truncation containing amino acids 1-61

    Promoter

    T7

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET303-ColE1-T-E192C

    Plasmid

    #180233

    Purpose

    Expresses T domain of Colicin E1 (residues 1-190) with C terminal cysteine for maleimide chemistry

    Depositor

    Joanna Slusky

    Article

    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

    Insert

    Colicin E1
    

    Tags

    6x His-tag

    Expression

    Bacterial

    Mutation

    Truncation of ColE1 containing T domains (residue…

    Promoter

    T7

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET15b LFn-Fla

    Plasmid

    #84871

    Purpose

    Bacterial expression of LFn-Fla fusion

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Inserts

    LFn
    Fla
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

22. LHP2691 - pET30-Ub-K63A

    Plasmid

    #32179

    Depositor

    Linda Hicke

    Insert

    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K63A

    Promoter

    T7

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

23. LHP2690 - pET30-Ub-K48A

    Plasmid

    #32178

    Depositor

    Linda Hicke

    Insert

    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K48A

    Promoter

    T7

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

24. pET21-pelB-LaG-18

    Plasmid

    #172763

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-18
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET23a-His-hRanGAP1tail

    Plasmid

    #53139

    Purpose

    E.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tag

    Depositor

    Frauke Melchior

    Article

    Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

    Insert

    RanGAP1 (RANGAP1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    C-terminal fragment (amino acids 398-587)

    Promoter

    T7

    Available Since

    May 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET26-MBP-GLIC

    Plasmid

    #20887

    Depositor

    Raimund Dutzler

    Article

    Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

    Insert

    pentameric ligand gated ion channel from Gloeobacter violaceus PCC 7421
    

    Tags

    His, MBP, and pelB

    Expression

    Bacterial

    Available Since

    Oct. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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27. pET28b-CoilR-Cys

    Plasmid

    #181989

    Purpose

    Used to label proteins with the CoilE genetically encoded tag

    Depositor

    Kimberly Beatty

    Article

    Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

    Insert

    CoilR-Cys
    

    Tags

    His-Tag

    Expression

    Bacterial

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET-45b-mCherry

    Plasmid

    #145279

    Purpose

    Bacterial Expression of 6xHis mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    mCherry
    

    Expression

    Bacterial

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28a-mCherry-2xEcMTS

    Plasmid

    #133624

    Purpose

    Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    mCherry-2xEcMTS
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. pET-51b-HaloTag11

    Plasmid

    #175519

    Purpose

    Expression of HaloTag11 in bacterial cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag11
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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