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pET His6 GST TEV LIC cloning vector (2G-T)

Plasmid

#29707

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, GST, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pCMV p16 INK4A

Plasmid

#10916

Depositor

Bob Weinberg

Publication

Medema et al Proc Natl Acad Sci U S A. 1995 Jul 3. 92

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

p16 INK4 (CDKN2A Human)

Expression

Mammalian

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3-FLAG-LKB1

Plasmid

#8590

Depositor

Lewis Cantley

Publication

Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LKB1 (STK11 Human)

Tags

FLAG

Expression

Mammalian

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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His6-TEV-CRBN-midi

Plasmid

#215330

Purpose

CRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader design

Depositor

Alessio Ciulli

Publication

Kroupova et al Nat Commun. 2024 Oct 15;15(1):8885. doi:

Insert

CRBN (CRBN Human)

Tags

6xHis and TEV

Expression

Bacterial

Mutation

contains amino acids 41-187 followed by a Gly-Ser…

Promoter

lacI

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-hIBA1a-GFP-miR124T

Plasmid

#214147

Purpose

AAV vector to restrict GFP expression in microglia.

Depositor

Chun-Li Zhang

Publication

Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

Has service

AAV5

Insert

GFP

Use

AAV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Cas12f-GE ver4.1

Plasmid

#176544

Purpose

Expresses Cas12f-GE in mammalian cells

Depositor

Yong-Sam Kim

Publication

Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Cas12f-GE ver4.1
Cas12f-GE ver4.1

Expression

Mammalian

Promoter

U6 and chicken β-actin

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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mNeonGreen-Giantin

Plasmid

#98880

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Giantin

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pKM464 (deletions)

Plasmid

#108322

Purpose

Orbit integrating plasmid to delete target gene.

Depositor

Kenan Murphy

Publication

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

Bxb1 attB site

Use

Orbit integration plasmid for creating knockouts …

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pC1-HyPer-C199S

Plasmid

#42213

Purpose

Peroxide-insensitive version of HyPer to be used as a pH-control. Can be applied as a pH sensor.

Depositor

Vsevolod Belousov

Publication

Belousov et al Nat Methods. 2006 Apr;3(4):281-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HYPER C199S

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pMYC-GFP

Plasmid

#42142

Depositor

Olufunmilayo Olopade

Publication

Chen et al BMC Cancer. 2011 Jun 13;11:246. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

c-Myc (MYC Human)

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hrGFP-Keap1

Plasmid

#28025

Depositor

Qing Zhong

Publication

Fan et al Autophagy. 2010 Jul 22. 6(5):.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Keap1 (KEAP1 Human)

Tags

hrGFP

Expression

Mammalian

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pMSCVpuro-Flag-cMyc T58A

Plasmid

#20076

Depositor

Juan Belmonte

Publication

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

cMyc (MYC Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

T58A

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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V51 pIRESpuro-GLUE (pGLUE)

Plasmid

#15100

Purpose

tandem-affinity purification plasmid; streptavidin-(streptag) and calmodulin-(CBP) binding affinity tags

Depositor

Randall Moon

Publication

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Type

Empty backbone

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCMV5-AML1B

Plasmid

#12426

Purpose

pCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C.

Depositor

Scott Hiebert

Publication

Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AML1B (RUNX1 Human)

Expression

Mammalian

Available since

Nov. 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCALNL-GFP

Plasmid

#13770

Purpose

Cre recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

EGFP

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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pTRIP-SFFV-mtagBFP-2A STING

Plasmid

#102586

Purpose

Lentivector to express STING and mTagBFP

Depositor

Nicolas Manel

Publication

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLVX-EGFP-IRES-puro

Plasmid

#128652

Purpose

mammalian expression of EGFP with puromycin selection

Depositor

Robert Sobol

Publication

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

EGFP

Use

Lentiviral

Tags

N/A

Expression

Mammalian

Mutation

Wild type

Promoter

CMV

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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3xnls-mScarlet-I

Plasmid

#98816

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

3xNLS

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCSII-EF-miRFP670v1-hGem(1/110)

Plasmid

#80006

Purpose

hGem(1/110) fused with near-infrared fluorescent protein miRFP670v1 (S/G2/M phase cell cycle reporter)

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

hGeminin (1/110) (GMNN Human)

Use

Lentiviral

Tags

miRFP670v1

Promoter

EF-1 alpha

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCAG_smFP V5

Plasmid

#59758

Purpose

encodes multiple copies of an epitope tag V5

Depositor

Loren Looger

Publication

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

smFP _V5

Expression

Mammalian

Promoter

CAG

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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