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Plasmid#74278PurposeConstitutive expression of CyOFP in bacteriaDepositorInsertCyOFP
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP-C-IFT52
Plasmid#218725PurposeExpresses N-terminally EGFP-tagged IFT52 in mammalian cellsDepositorInsertIFT52 (IFT52 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3 basic Mut E2
Plasmid#48748PurposeExpression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1)DepositorInsertmPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
UseLuciferaseTagsExpressionMutationMutated E-box2 sequence from CACGTT to GCTAGTPromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-control-APEX
Plasmid#73187PurposeExpresses "control"-APEX (cytosolic) in mammalian cellsDepositorInsertcontrol-APEX (Nphp3 Glycine max, Mouse)
UseTagsinternal GFP tagExpressionMammalianMutationchanged glycine 2 to alaninePromoterEF1alphaAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-SAKS1-Strep-HA
Plasmid#113487PurposeExpression of human SAKS1 with C-terminal strep-HA tagDepositorInsertSAKS1 (UBXN1 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcpBpaRS_v2
Plasmid#196485PurposeTo express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.DepositorInsertbenzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
UseTagsExpressionMammalianMutationPromoterSV40(synthetase), Pol III tRNA promoter (tRNA), C…Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-GFP-αTAT1[D157N]
Plasmid#27100DepositorInsertαTubulin K40 acetyltransferase (ATAT1 Human)
UseTagsGFP and S-tagExpressionMammalianMutationD157NPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-EGFP-SOS1
Plasmid#178860PurposeMammalian expression of SOS1 fused to EGFPDepositorInsertSOS1 (SOS1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST39-pNL29
Plasmid#91696PurposeExpresses Mega GVs in E.ColiDepositorInsertMega GV gene cluster (from pNL29) in pST39 backbone
UseTagsNo tagsExpressionBacterialMutationPromoterT7Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
4LNDDE488Q-pCDNA3.1(+)
Plasmid#172228PurposeExpresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) in mammalian cellsDepositorInsertLambda N peptide - human ADAR2 Deaminase Domain E488Q
UseTags6xHis and FlagExpressionMammalianMutationchanged the Glutamic acid 488 in the Deaminase Do…PromoterCMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SypHer2-DAAO-NES
Plasmid#119184PurposeFusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.DepositorInsertSypHer2-DAAO-NES
UseAAVTagsNESExpressionMammalianMutationPromoterCMVAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHT-6xR.ads-PxylA-DI
Plasmid#47386PurposeExpresses ads under Pgrac promoter (inducible by IPTG) and expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteriaDepositorInsertsads
dxs
idi
UseTags6xR and HisExpressionBacterialMutationPromoterPgrac and PxylAAvailable sinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast
Plasmid#223173PurposeExpresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.DepositorInserthfCas13d (N2V8)
UseLentiviralTagsNLS-HAExpressionMutationPromoterAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT-EF-Pdgfralpha-EGFPN D842V
Plasmid#66789PurposeExpression of constitutively active murine PDGFRalpha D842V tagged with GFP under the control of EF1a promoterDepositorInsertPDGFRalpha (Pdgfra Mouse)
UseTagsEGFPExpressionMammalianMutationD842V (constitutively active)PromoterEF1aAvailable sinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcpAzpaRS_v2
Plasmid#196483PurposeTo express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.DepositorInsertazidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
UseTagsExpressionMammalianMutationPromoterSV40(synthetase), Pol III tRNA promoter (tRNA), C…Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IB-mGFP-FTH1
Plasmid#192800PurposeStable expression of mGFP-FTH1 in mammalian cellsDepositorInsertFTH1 (FTH1 Human)
UseTagsmonomeric EGFP (A206K)ExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody
Plasmid#138132PurposeExpresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.DepositorInsertEGFP-anti-mouse-IgG(kappa)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB-Ric8b-Integration
Plasmid#129457PurposepiggyBac transposon vector that Inducibly (Tet-On) expresses Ric8bDepositorInsertRic8b (Ric8b Mouse)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE (Tet-On)Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-His-MBP-NLS-RfxCas13d-NLS-HA
Plasmid#171586PurposeBacterial expression of NLS-RfxCas13d-NLS-HADepositorInsertHis-MBP-NLS-RfxCas13d-NLS-HA
UseTagsHAExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBV65
Plasmid#108363PurposeA nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.DepositorInsertloxP-natMX6-CgMET3prom-cre-loxP
UseGene disruption marker for candida glabrataTagsExpressionMutationPromoterC. glabrata MET3Available sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only