159,237 results

1. pRSF-G1(7AW)RS

   Plasmid

   #207516

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

   Insert

   tRNA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mCherry-Vav2

   Plasmid

   #129623

   Purpose

   RhoGEF

   Depositor

   Dorus Gadella

   Article

   Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

   Insert

   Vav2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-flag-YTHDF2-N

   Plasmid

   #52302

   Purpose

   Flag-tagged YTHDF2 N-terminal domain, made by mutating to E384(GAA) to stop codon(TAA)

   Depositor

   Chuan He

   Article

   Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

   Insert

   YTHDF2 (YTHDF2 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Changed E384 to stop codon

   Promoter

   T7

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

4. CAG-Flex-RG

   Plasmid

   #48333

   Purpose

   Cre-dependant (flex) rabies glycoprotein

   Depositor

   Liqun Luo

   Article

   Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 1

   Insert

   Rabies Glycoprotein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDestTol2-ubi-ASAP1

   Plasmid

   #121987

   Purpose

   ubiqutously express ASAP1 voltage biosensor

   Depositor

   GuangJun Zhang

   Article

   Silic et al J Vis Exp. 2018 Apr 25;(134). doi: 10.37

   Insert

   ASAP1
   

   Use

   Tol2 transposon destination vector

   Tags

   Expression

   Mutation

   Promoter

   zebrafish ubiquitin promoter

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJMP2844

   Plasmid

   #160675

   Purpose

   Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

   Depositor

   Jason Peters

   Article

   Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBa.NgCAM-mCherry

   Plasmid

   #180248

   Purpose

   Mammalian expression of NgCAM

   Depositor

   Marvin Bentley

   Article

   Nabb et al Mol Biol Cell. 2021 Nov 3:mbcE21080425.

   Insert

   NgCAM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Chicken Beta actin

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Gal:mCherry-uTEV3

   Plasmid

   #135453

   Purpose

   Yeast expression of uTEV3 fused to mCherry

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   mCherry-uTEv3
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   S219V mutation improves stability , I138T/S153N/T…

   Promoter

   Gal

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AiP14253 - pAAV-AiE0380h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4253)

   Plasmid

   #208165

   Purpose

   AiE0380h_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytes

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Article

   Mich et al bioRxiv 2023.09.20.558718

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Beta Globin minimal promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CL7-LbCas12a

    Plasmid

    #164658

    Purpose

    Bacterial expression of LbCas12a using CL7/Im7

    Depositor

    Piyush Jain

    Article

    Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

    Insert

    LbCas12a
    

    Use

    Tags

    Thioredoxin, CL7, SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHyVec11

    Plasmid

    #34794

    Depositor

    Rob Steele

    Type

    Empty backbone

    Use

    Hydra expression

    Tags

    Intergenic region between Hydra replication facto…

    Expression

    Mutation

    Promoter

    Hydra vulgaris actin promoter

    Available since

    Jan. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMXs_FLAG-MCART1

    Plasmid

    #133247

    Purpose

    Express FLAG-tagged MCART1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    MCART1 (SLC25A51 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells and…

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP-N1-Letm1

    Plasmid

    #191937

    Purpose

    Expression of a Letm1 (Leucine Zipper And EF-Hand Containing Transmembrane Protein 1)- GFP chimera.

    Depositor

    Luca Scorrano

    Insert

    Letm1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRN119

    Plasmid

    #176884

    Purpose

    Encodes STM and WUS2 for ectopic meristem induction in plants

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    CmYLCV:Luc+:HSP, Nos::WUS2:pinII, and 35:STM:35S
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mPA-GFP-N1

    Plasmid

    #54712

    Purpose

    Localization: N1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mPA-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pEE083

    Plasmid

    #149279

    Purpose

    T-DNA encoding TRV2 complete genome with MCS

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AiP13568 - pAAV-AiE0387m_3xC1-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3568)

    Plasmid

    #208142

    Purpose

    AiE0387m_3xC1 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytes

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Mich et al bioRxiv 2023.09.20.558718

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mVenus-UbiquitinC-N-18

    Plasmid

    #56339

    Purpose

    Localization: Membrane , Excitation: 515, Emission: 528

    Depositor

    Michael Davidson

    Insert

    UbiquitinC (UBA52 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    aa 2-76 of NP_003324.1

    Promoter

    CMV

    Available since

    Dec. 18, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pcDNA3-flag-YTHDF2-C

    Plasmid

    #52303

    Purpose

    Flag-tagged YTHDF2 C-terminal domain, from E384 to the end

    Depositor

    Chuan He

    Article

    Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-383

    Promoter

    T7

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMK453 (BSD-P2A-dTAG-mCherry)

    Plasmid

    #214383

    Purpose

    dTAG-mCherry for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BSD-P2A-dTAG-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge