165,775 results

1. pTUM4

   Plasmid

   #230905

   Purpose

   Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli

   Depositor

   Arne Skerra

   Article

   Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-

   Inserts

   FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
   SurA (surA E. coli)
   DsbA (dsbA E. coli)
   DsbC (dsbC E. coli)
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1 mCherry

   Plasmid

   #128073

   Purpose

   puro exchanged to mCherry

   Depositor

   Oskar Laur

   Insert

   mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   no

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas9-KRAB-MeCP2

   Plasmid

   #110821

   Purpose

   Improved dCas9 repressor-dCas9-KRAB-MeCP2

   Depositor

   Alejandro Chavez, George Church

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB-MeCP2
   

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKM208

   Plasmid

   #13077

   Depositor

   Kenan Murphy

   Article

   Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

   Insert

   lacI repressor (lacI E. coli)
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pV231-xPE

   Plasmid

   #225257

   Purpose

   Mammalian expression of xPE

   Depositor

   Vikash Chauhan, Robert Langer, Phil Sharp

   Article

   Chauhan et al Nature. 2025 Sep 17. doi: 10.1038/s41586

   Insert

   xPE
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   R221K K848A H982A N1317R

   Promoter

   CMV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET His6 MBP TEV LIC cloning vector (1M)

   Plasmid

   #29656

   Depositor

   Scott Gradia

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   6xHis, MBP, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-mito-R-GECO1

   Plasmid

   #46021

   Purpose

   Red intensiometric genetically encoded Ca2+-indicators for optical imaging

   Depositor

   Robert Campbell

   Article

   Wu et al ACS Chem Neurosci. 2013 Mar 19.

   Insert

   R-GECO1
   

   Tags

   a duplex of the mitochondrial targeting signal of…

   Expression

   Mammalian

   Mutation

   Substitutions relative to the mApple-derived anal…

   Promoter

   CMV

   Available Since

   July 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EF1A-CD19.28H.28TM.28.BB.3z

   Plasmid

   #200680

   Purpose

   Modular CD28hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Michele Bernasconi

   Article

   Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

   Insert

   FMC63-CD28HD-CD28TM-CD28-41BB-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)
   

   Use

   Lentiviral

   Tags

   MycTag

   Expression

   Mammalian

   Promoter

   EF1a-short

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBeloBac11loxP2272

   Plasmid

   #60342

   Purpose

   This is a BAC vector that contains both wt and mutant loxP sites.

   Depositor

   Jon Coren

   Article

   Coren et al BMC Res Notes. 2017 Jul 28;10(1):344. do

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Bacterial

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lentiviral-TOP-dGFP-reporter

    Plasmid

    #14715

    Purpose

    Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).

    Depositor

    Tannishtha Reya

    Article

    Reya et al Nature. 2003 May 22. 423(6938):409-14.

    Insert

    LEF-1/TCF-responsive promoter (Ctnnb1 )
    

    Use

    Lentiviral

    Tags

    d2 eGFP

    Expression

    Mammalian

    Available Since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmCherry-MS2coatProtein

    Plasmid

    #103832

    Purpose

    Live cell reader for MS2 loop RNA production

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    mCherry-MS2 coat protein (cp )
    

    Expression

    Mammalian

    Mutation

    MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB_TRE3G_dCas9-5xGCN4_Hygro

    Plasmid

    #235572

    Purpose

    Dox-inducible expression of dCas9-GCN4 & genomic integration

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    dCas9-5xGCN4
    

    Use

    CRISPR

    Mutation

    D10A; H840A

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFA6a-link-yoEGFP-CaURA3

    Plasmid

    #44872

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized EGFP with CaUra3 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoEGFP
    

    Expression

    Yeast

    Available Since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. tdTomato-N1

    Plasmid

    #54642

    Purpose

    Localization: N1 Cloning Vector, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson, Nathan Shaner, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGP-CMV-NES-jRCaMP1a

    Plasmid

    #61562

    Purpose

    Red fluorescent calcium sensor protein

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Insert

    NES-jRCaMP1a
    

    Tags

    nuclear export signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSBbi-GH

    Plasmid

    #60514

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and hygromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMH-SFB

    Plasmid

    #99391

    Purpose

    Gateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)

    Depositor

    Michael Huen

    Article

    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Type

    Empty backbone

    Tags

    SFB (S protein-FLAG-Streptavidin binding peptide)

    Expression

    Mammalian

    Available Since

    Sept. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. OpenCRISPR-1 sgRNA-16nt stem

    Plasmid

    #221566

    Purpose

    Express OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

    Depositor

    Ali Madani

    Article

    Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

    Insert

    OpenCRISPR-1 sgRNA 16nt stem loop
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pMCB320

    Plasmid

    #89359

    Purpose

    3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    puromycin resistance, mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-VSVG

    Plasmid

    #11912

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

    Insert

    VSVG
    

    Expression

    Mammalian

    Mutation

    F64L, S65T, A206K relative the wild type GFP sequ…

    Available Since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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