Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 17,487 results for: Emb

Clear keywords
Showing: 2681 - 2720 of 17487 results

  3. LifeAct-14 (LA-14)

    Plasmid
    #158750
    Purpose
    Lentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.
    Depositor
    Minhajuddin Sirajuddin
    Article
    Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152
    Insert
    LifeAct-14
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Deletion mutation for last three amino acids from…
    Promoter
    CMV immediate early
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158750-plasmid-map-sequence-id-310555

    Enlarge

  15. pLenti-Tet-GBP1-C589A

    Plasmid
    #138090
    Purpose
    Lentiviral backbone for inducible expression of human GBP1 missing the reactive cysteine of the CaaX-box (GBP1-C589A)
    Depositor
    Eva Frickel
    Article
    Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:
    Insert
    human GBP1 (GBP1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    cysteine 589 to alanine (C589A)
    Promoter
    minimal CMV promoter with heptamerized tet‐operat…
    Available Since
    April 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    138090-plasmid-map-sequence-id-276307

    Enlarge

  21. pAV10.F3

    Plasmid
    #63199
    Purpose
    BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    63199-plasmid-map-sequence-id-108468

    Enlarge

  28. pAV10.KH

    Plasmid
    #63208
    Purpose
    pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    63208-plasmid-map-sequence-id-104909

    Enlarge

  31. pCDNA-CRY2-mCh-superPLDx10-P2A-CIBN-CAAX

    Plasmid
    #188991
    Purpose
    A plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBN
    Depositor
    Jeremy Baskin
    Article
    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415
    Insert
    PLD
    Tags
    CAAX, CIBN, CRY2, P2A, and mCherry
    Expression
    Mammalian
    Mutation
    F163L, K249R, G328S, G381V, G429D, D480G
    Promoter
    CMV
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    188991-plasmid-map-sequence-id-371644

    Enlarge

  36. pCAG-lox-rox-STOP-rox-lynScarlet-lox-Lyn-NeonGreen

    Plasmid
    #175259
    Purpose
    Dual plasmid labeling membrane target protein of different neuronal population with different fluorophore
    Depositor
    Frank Bradke, Sebastián Dupraz
    Article
    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37
    Insert
    Lox-rox-STOP-rox-lyn-mScarlet-pA-lox
    Use
    Cre/Lox; Dre/rox
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    None (wt)
    Promoter
    pCAG
    Available Since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    175259-plasmid-map-sequence-id-375583

    Enlarge

Showing: 2681 - 2720 of 17487 results