We narrowed to 5,067 results for: codon optimized
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pDuRCC-N
Plasmid#81194PurposeExpression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease view depositor comments below and WT - cod…PromoterROX3 and SNP52pAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
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MSP1594
Plasmid#65775PurposeHuman expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLSDepositorInsertmammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
UseCRISPRTagsNLSExpressionMammalianMutationPromoterCAGAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/MSCV-EGFP
Plasmid#82613PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseRetroviralTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMutationhuman codon-optimized, D10A + H840APromoterLTRAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(800-868)
Plasmid#141345PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868).DepositorInsertGB1His6-TEV-ALIX(800-868)
UseTagsGB1-His6-TEVExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
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SpRY
Plasmid#198544PurposeExpresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSDDepositorInsertSpRY
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-PsACR1
Plasmid#85465PurposeNatural anion conducting Channelrhodopsin with red shifted absorption. Closing kinetics 80ms. Codon optimized for mammalian expression (human/mouse)DepositorInsertSynthetic construct PsChR1 gene
UseTagsmCherryExpressionMammalianMutationPromoterCMV (+enhancer)Available sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUDE1093
Plasmid#204227PurposeEpisomal yeast plasmid expressing the Ercas12aDepositorInsertEubacterium rectale cas12a (Ercas12a)
UseTagsExpressionYeastMutationcodon optimized for S. cerevisiaePromoterSaccharomyces cerevisiae PGK1Available sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-CPZ-TAN3 vpr
Plasmid#115833PurposeEncodes codon optimized 3xFLAG-SIVCPZ-TAN3 Vpr (DQ374658) and puromycin resistance proteinDepositorInsertSIVCPZ-TAN3 Vpr (DQ374658)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-CPZ-LB7 vpr
Plasmid#115834PurposeEncodes codon optimized 3xFLAG-SIVCPZ-LB7 Vpr (DQ373064) and puromycin resistance proteinDepositorInsertSIVCPZ-LB7 Vpr (DQ373064)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-GOR -vpr
Plasmid#115835PurposeEncodes codon optimized 3xFLAG-SIVGOR Vpr (FJ424871) and puromycin resistance proteinDepositorInsertSIVGOR Vpr (FJ424871)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
AID-mTagBFP2-loxP_myo2_neoR-loxP
Plasmid#194055PurposeDual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genomeDepositorInsertIAA17 (AXR3 Mustard Weed)
UseTagscodon optimized mTagBFP2ExpressionWormMutationPromoterNoneAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pCas9-VRER_2A_GFP
Plasmid#75475PurposeCas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression controlDepositorInsertCas9-VRER
UseCRISPRTags2A_GFPExpressionMammalianMutationD1135V, G1218R, R1335E, T1337RPromoterCAGAvailable sinceMay 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
UMG-SP-1_pET29b
Plasmid#215433PurposeExpression construct for UMG-SP-1 gene, codon optimized for expression in E. coliDepositorInsertUMG-SP-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
UMG-SP-2_pET29b
Plasmid#215434PurposeExpression construct for UMG-SP-2 gene, codon optimized for expression in E. coliDepositorInsertUMG-SP-2
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-efSaCas9-BlastR
Plasmid#220971PurposeExpresses human codon-optimized efSaCas9DepositorInsertefSaCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVRc20_992
Plasmid#49739DepositorInsertAS20_992
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT3-Neo
Plasmid#69123PurposeSleeping Beauty (SB) transposon with G418 resistanceDepositorInsertNeo (codon optimized for human)
UseSleeping beauty transposonTagsExpressionMammalianMutationPromoterU3MSCVAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
NylB_pET29b
Plasmid#215414PurposeExpression construct for NylB gene, codon optimized for expression in E. coliDepositorInsertNylB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-bphC
Plasmid#128137PurposeThe cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertbphC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rhadCas9-native
Plasmid#129392PurposeDerived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.DepositorInsertS. pyogenes dCas9
UseCRISPRTagsExpressionBacterialMutationAspartate 10 to Alanine, Histidine 840 to AlaninePromoterPrhaBADAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRAN473
Plasmid#120813PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target proteinDepositorInsertred fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSCL.028
Plasmid#184965PurposeTest effect of extending -Eco1 a1/a2 in yeastDepositorInsertEco1: RT and ncRNA(extended), a1/a2 length: 27
UseTagsExpressionYeastMutationhuman codon optimized RT, a1/a2 length extended t…PromoterGal7Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pscALPS SIV-MND2 vpx
Plasmid#115811PurposeEncodes codon optimized 3xFLAG-SIVMND2 Vpx (AY159322) and puromycin resistance proteinDepositorInsertSIVMND2 Vpx (AY159322)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJC30X-BS(X)
Plasmid#58958PurposeEncodes synthetic mitochondrial gene GFPm-3 in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.DepositorInsertEnhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code
UseTagsFirst 8 codons of COX3ExpressionMutationGFP modified as described: CORMACK, B. P., R. H. …Promotern/aAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
ALIX(P801G)-Strep
Plasmid#180024PurposeCodon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.DepositorInsertALIX(1-868, P801G)-Strep (PDCD6IP Human)
UseTagsStrep tag (WSHPQFEK)ExpressionBacterialMutationP801GPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKMD106h - intein-Cas9(C574)
Plasmid#64191PurposeExpresses intein-Cas9(C574) in mammalian cellsDepositorInsertmammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG
UseCRISPRTagsNLS - 3x FLAG (C terminal on insert)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
TOPO rtTA3-2A-Cre
Plasmid#68448PurposeTOPO construct expressing rtTA3-2A-Cre for generation of GMAP-compatible geneDepositorInsertrtTA3-2A-Cre
UseGmapTagsExpressionBacterialMutationCodon optimized for expression in micePromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tt-NylC_pET28a
Plasmid#215431PurposeExpression construct for Tt-NylC gene, codon optimized for expression in E. coliDepositorInsertTt-NylC
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSP2283
Plasmid#70702PurposeBacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgL-FLAG (Merlin)
Plasmid#136450PurposeMammalian expression plasmid for HCMV glycoprotein L (Merlin strain) with FLAG tagDepositorInsertglycoprotein L (UL115 Human betaherpesvirus 5)
UseTagsShort SGGSG linker and FLAG tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pco-vCASphi_E9t_MCS_version2
Plasmid#197961PurposeT-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter.DepositorInsertpco-vCasphi-2
UseCRISPRTagsExpressionPlantMutationPromoterpUBQ10Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pco-nCASphi_E9t_MCS_version2
Plasmid#197962PurposeT-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter.DepositorInsertpco-nCasphi-2
UseCRISPRTagsExpressionPlantMutationPromoterpUBQ10Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVRc03_1198
Plasmid#49730DepositorInsertAS03_1198
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FLAG- SIV-MAC251-vpx-Q76A
Plasmid#115843PurposeEncodes codon optimized FLAG tagged SIVMAC251-Vpx-Q76A mutantDepositorInsertSIVMAC251-Vpx-Q76A
UseTags3x-FLAGExpressionMammalianMutationQ76APromoterCMVAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP
Plasmid#45578PurposeExpresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP trackerDepositorInsertcoY2 Ani
UseLentiviralTagsHA, IRES BFP, and NLSExpressionMammalianMutationco indicates codon optimization for mammalian exp…PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-RCM vpx
Plasmid#115812PurposeEncodes codon optimized 3xFLAG-SIVRCM Vpx (AF349680) and puromycin resistance proteinDepositorInsertSIVRCM Vpx (AF349680)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only
