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pHR_D17_hrGFP
Plasmid#84616PurposeHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into D17 locusDepositorInserthrGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterUAS1B8-TEF(136)Available sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_AXP_hrGFP
Plasmid#84613PurposeHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locusDepositorInserthrGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterUAS1B8-TEF(136)Available sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_MFE1_hrGFP
Plasmid#84617PurposeHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locusDepositorInserthrGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterUAS1B8-TEF(136)Available sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAT112TAG-SepT
Plasmid#34624DepositorInserttRNA^Sep
UseTagsExpressionBacterialMutationPromoterlppAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHR_A08_hrGFP
Plasmid#84615PurposeHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into A08 locusDepositorInserthrGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterUAS1B8-TEF(136)Available sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_XPR2_hrGFP
Plasmid#84614PurposeHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into XPR2 locusDepositorInserthrGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterUAS1B8-TEF(136)Available sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMEG_BB3_YL001
Plasmid#117825PurposeOverexpession plasmid of glycerol kinase (GUT1) in Y. lipolyticaDepositorInsertYlpTEF_YlGUT1_YlCyc1TT
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICH82094
Plasmid#48074DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 3, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNK141l2
Plasmid#156385PurposeExpresses orthogonal RBS LSS-mKate2 reporterDepositorInsertLSS-mKate2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCyc-Bem1
Plasmid#66573PurposeExpress an additional copy of Bem1, integrated at TRP locusDepositorInsertBem1
UseTagsExpressionYeastMutationF99I*(see below)PromoterpCyc1Available sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMEG_YLCas9_empty
Plasmid#117826PurposeCRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolyticaDepositorInsertYlpTEF_hCas9_YlCyc1TT
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEDJ333
Plasmid#177272PurposeGAL1p:Cas9:CYC1tDepositorInsertCas9
UseTagsExpressionBacterial and YeastMutationPromoterGAL1Available sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pACλCI
Plasmid#53730PurposePlacUV5-directed synthesis of the λCI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733.DepositorInsertbacteriophage lambda CI protein
UseTagsExpressionBacterialMutationPromoterlac UV5Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCfB4170
Plasmid#126750PurposeExpresses pTEF2-mKate2-tIDP1 and pTRX2_5xUAS-yEGFP-tCYC1 in yeast from plasdmid pRS413DepositorInsertyEGFP
UseTagsExpressionMutationPromoterpTRX2_5xUASAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
PPDK
Plasmid#64037PurposeExpression of Pyruvate phosphate dikinase (PPDK) in E. coliDepositorInsertpyruvate phosphate dikinase
UseTagsnoneExpressionBacterialMutationPromoterAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB3236
Plasmid#124708PurposeExpresses pTEF2-mKate2-tIDP1 and pTRX2_5xUAS-yEGFP-tCYC1 in yeast from plasdmid pRS416DepositorInsertyEGFP
UseTagsExpressionMutationPromoterpTRX2_5xUASAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMLB10
Plasmid#177273PurposeGAL1p:T7RNAP(F11L;T613A):CYC1tDepositorInsertT7RNAP(F11L;T613A)
UseTagsExpressionBacterial and YeastMutationF11L T613APromoterGAL1Available sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
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KvAvs3
Plasmid#188792PurposeAvs3 from Klebsiella variicola PF-1DepositorInsertKvAvs3
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
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Avs4_Sulfurospirillum_sp.
Plasmid#188801PurposeAvs4 from Sulfurospirillum sp.DepositorInsertAvs4
UseTagsExpressionBacterialMutationPromoterEcAvs4 native promoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
SsAvs4
Plasmid#188799PurposeAvs4 from Streptomyces sp. A244DepositorInsertSsAvs4
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKP1193
Plasmid#167614PurposeExpress TonB H20NDepositorInserttonB H20N
UseTagsExpressionBacterialMutationtonB with H20N mutationPromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP381
Plasmid#167592PurposeExpress TonB H20A proteinDepositorInserttonB H20A
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCas9
Plasmid#42876PurposeBacterial expression of Cas9 nuclease, tracrRNA and crRNA guideDepositorInserttracr/Cas9
UseCRISPR; E.coliTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLG001 (empty vector)
Plasmid#157879PurposeEmpty vector backbone for defense systemsDepositorInsertNone
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMJH65
Plasmid#67273PurposeConjugally transferable red recombinogenic plasmidDepositorInserttetA
UseRed recombinase, temperature sensitiveTagsExpressionBacterialMutationPromotertetA promoter of plasmid pACYC184 originAvailable sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
SeAvs3
Plasmid#188791PurposeAvs3 from Salmonella enterica NCTC13175DepositorInsertSeAvs3
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ec2Avs2
Plasmid#188790PurposeAvs2 from Escherichia coli NCTC10650DepositorInsertEc2Avs2
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
EcAvs4
Plasmid#188794PurposeAvs4 from Escherichia coli NCTC11132DepositorInsertEcAvs4
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only
