Skip to main content
Addgene
Showing: 241 - 280 of 337 results
  1. pMTMVi-M

    Plasmid
    #118757
    Purpose
    Intermediate plasmid to build tobacco mosaic virus recombinant clones that move systemically throughout the plant.
    Depositor
    Insert
    Tobacco mosaic virus cDNA fragment with a cloning polylinker
    Use
    Intermediate to build tobacco mosaic virus recomb…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pMTMVi-N

    Plasmid
    #118756
    Purpose
    Intermediate plasmid to build tobacco mosaic virus recombinant clones that do not move systemically throughout the plant.
    Depositor
    Insert
    Tobacco mosaic virus cDNA fragment with a cloning polylinker
    Use
    Intermediate to build tobacco mosaic virus recomb…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. EcoHIV-mScarlet

    Plasmid
    #235598
    Purpose
    EcoHIV-mScarlet is a modification of pNL4-3-EcoHIV (Potash et al, PNAS 2005). EcoHIV is rodent-infectable modification of HIV where HIV env replaced for MLV-env
    Depositor
    Insert
    NL4-3 EcoHIV mScarlet
    Use
    Lentiviral
    Tags
    mScarlet
    Expression
    Mutation
    Promoter
    Available since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. p5’UTR-ORF1

    Plasmid
    #190566
    Purpose
    Holding vector containing the contiguous 5’UTR and ORF1 regions of human L1 (L1.3) to facilitate subcloning of ORF1 mutants into the full-length L1 reporter pRTC2-puro (Cook et al., 2015).
    Depositor
    Insert
    Contiguous regions of the human L1 (L1.3) 5’UTR and ORF1
    Use
    For dna propagation only
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    N/A
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pVR2-m2

    Plasmid
    #84721
    Purpose
    pVR2 but with a G to U mutation in the riboswitch aptamer
    Depositor
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G to U mutation at position 16 of annotated "…
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. pVR2-m3

    Plasmid
    #84722
    Purpose
    pVR2 but with a C to G mutation in the riboswitch aptamer
    Depositor
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C to G mutation at position 22 of annotated "…
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. pVR

    Plasmid
    #84718
    Purpose
    E. coli RecA promoter followed by preQ1 riboswitch from the 5' UTR of the queC gene from B. subtilis
    Depositor
    Inserts
    E. coli RecA promoter
    5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. pVR2-m1

    Plasmid
    #84720
    Purpose
    pVR2 but with a C to U mutation in the riboswitch aptamer
    Depositor
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C to U mutation at position 13 of annotated "…
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. pTL-Bag3-gRNA3

    Plasmid
    #196254
    Purpose
    Plasmid for cloning the third CRISPR-Cas9 guide RNA of 4 guide RNAs
    Depositor
    Insert
    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. pTL-Bag2-gRNA2

    Plasmid
    #196252
    Purpose
    Plasmid for cloning the second CRISPR-Cas9 guide RNA of multiplex guide RNAs
    Depositor
    Insert
    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. pTL-Bag1-gRNA1

    Plasmid
    #196251
    Purpose
    Plasmid for cloning the first CRISPR-Cas9 guide RNA of multiplex guide RNAs
    Depositor
    Insert
    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  12. pTL-Bag4-gRNA4

    Plasmid
    #196255
    Purpose
    Plasmid for cloning the 4th CRISPR-Cas9 guide RNA of 4 guide RNAs
    Depositor
    Insert
    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  13. pGfa28-nLac

    Plasmid
    #53130
    Purpose
    Expression of LacZ driven by partial human gfa2 promoter, expression is localized to a subset of astrocytes in mice.
    Depositor
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    contains the A and B regions (bp -1757 to -1489) …
    Promoter
    human Gfa2
    Available since
    July 30, 2014
    Availability
    Academic Institutions and Nonprofits only
  14. pVR2-m4

    Plasmid
    #84723
    Purpose
    pVR2 but with G to U and A to C mutations following the riboswitch aptamer
    Depositor
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G to U mutation at the first nucleotide after the…
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  15. pVR2-m5

    Plasmid
    #84724
    Purpose
    pVR2 but with an insertion of 1 U immediately following the riboswitch aptamer
    Depositor
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    insertion of 1 U between positions 39 and 40 of a…
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  16. pVR2-m6

    Plasmid
    #84725
    Purpose
    pVR2 but with an insertion of 2 U's immediately following the riboswitch aptamer
    Depositor
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    insertion of 2 Us between positions 39 and 40 of …
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  17. pVR2-m7

    Plasmid
    #84726
    Purpose
    pVR2 but with a G to U mutation immediately following the riboswitch aptamer
    Depositor
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G to U mutation at position 41 of annotated "…
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  18. pVR2-m8

    Plasmid
    #84727
    Purpose
    pVR2 but with two G to U mutations immediately following the riboswitch aptamer
    Depositor
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G to U mutations at positions 40 and 41 of annota…
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  19. pVR2

    Plasmid
    #84719
    Purpose
    E. coli RecA promoter followed by stretch with no A's followed by riboswitch for single-round transcription
    Depositor
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    12 nt stretch lacking A's inserted before 5&…
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  20. pGfa2-nLac

    Plasmid
    #53126
    Purpose
    Expression of LacZ driven by the human Gfa2 promoter. LacZ can also be replaced with the gene of interest for targeting transgene expression to astrocytes in mice.
    Depositor
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    contains -2163 to +47 (the human gfa2 segment), w…
    Promoter
    human GFAP
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 241 - 280 of 337 results