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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,192 results for: Hras;

Showing: 301 - 350 of 1192 results

  1. pJYDC2

    Plasmid
    #162459
    Purpose
    Yeast display plasmid for expression of C-terminal fusions. Modification of pJYDC1 vector with ALFA tag in MCS.
    Depositor
    Gideon Schreiber
    Article
    ZahradnĂ­k et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA-tag, HA tag, HDEL sequence, and eUnaG2 fluor…
    Expression
    Mutation
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162459-plasmid-map-sequence-id-321477

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  3. pJYDC1

    Plasmid
    #162458
    Purpose
    Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter, eUnaG2 bilirubin dependent green-yellow fluorescent protein, is fused to the Nterminus
    Depositor
    Gideon Schreiber
    Article
    ZahradnĂ­k et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA tag, HDEL sequence, eUnaG2 fluorescent protein…
    Expression
    Mutation
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162458-plasmid-map-sequence-id-321474

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  4. pJYDN

    Plasmid
    #162450
    Purpose
    Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.
    Depositor
    Gideon Schreiber
    Article
    ZahradnĂ­k et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    Aga2p, HA, myc tags, and eUnaG2 fluorescent prote…
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162450-plasmid-map-sequence-id-321442

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  5. pJYDN3

    Plasmid
    #162456
    Purpose
    Yeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.
    Depositor
    Gideon Schreiber
    Article
    ZahradnĂ­k et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…
    Expression
    Mutation
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162456-plasmid-map-sequence-id-321464

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  6. pJYDN2

    Plasmid
    #162454
    Purpose
    Yeast display plasmid intended for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA) and expression of N-terminal fusions with Aga2p yeast agglutinin.
    Depositor
    Gideon Schreiber
    Article
    ZahradnĂ­k et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    DnbALFA nanobody and Ha and myc
    Expression
    Mutation
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162454-plasmid-map-sequence-id-321461

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  7. pJYDN2p

    Plasmid
    #162455
    Purpose
    Positive control plasmid for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA).
    Depositor
    Gideon Schreiber
    Article
    ZahradnĂ­k et al bioRxiv Preprint 2020
    Insert
    Aga2p-DnbALFA (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    DnbALFA nanobody and HA and myc
    Expression
    Mutation
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162455-plasmid-map-sequence-id-321462

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  8. pJYDNgp

    Plasmid
    #162453
    Purpose
    Positive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker.
    Depositor
    Gideon Schreiber
    Article
    ZahradnĂ­k et al bioRxiv Preprint 2020
    Insert
    Aga2p-2Glinker-eUnaG2 (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA nad myc and eUnaG2 fluorescent protein
    Expression
    Mutation
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162453-plasmid-map-sequence-id-321460

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  9. pJYDN3p

    Plasmid
    #162457
    Purpose
    Positive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.
    Depositor
    Gideon Schreiber
    Article
    ZahradnĂ­k et al bioRxiv Preprint 2020
    Insert
    Aga2p-eUnaG2 (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA tag, HA and myc tags, and eUnaG2 fluorescent…
    Expression
    Mutation
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162457-plasmid-map-sequence-id-321472

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  14. APRT

    Plasmid
    #64028
    Purpose
    Expression of S. crervisiae strain APRTase in E. coli. Contains N-terminal HistidineX6 tag.
    Depositor
    Vern Schramm
    Article
    Shi et al Biochemistry. 2001 Sep 11;40(36):10800-9
    Insert
    adenine phosphoribosyltransferase (APT1 Budding Yeast)
    Use
    Tags
    Histidine x 6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. PPDK

    Plasmid
    #64037
    Purpose
    Expression of Pyruvate phosphate dikinase (PPDK) in E. coli
    Depositor
    Vern Schramm
    Article
    Sturm et al Anal Chem. 2009 Apr 15;81(8):2847-53. do
    Insert
    pyruvate phosphate dikinase
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2015
    Availability
    Academic Institutions and Nonprofits only

  32. PNMT

    Plasmid
    #64078
    Purpose
    Expression of human Phenylethanolamine N-methyltransferase
    Depositor
    Vern Schramm
    Article
    Stratton et al ACS Chem Biol. 2017 Feb 17;12(2):342-346
    Insert
    Phenylethanolamine N-methyltransferase (PNMT Synthetic, Human)
    Use
    Tags
    Histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only

  39. pSM046

    Plasmid
    #217400
    Purpose
    KmR, pBTRcK broad host range vector with BTE2(C12-specific fatty acid thioesterase codon-optimised for C. necator) under Ptrc
    Depositor
    Brian Pfleger
    Article
    Mishra et al J Ind Microbiol Biotechnol. 2024 Feb 16:
    Insert
    BTE2
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PTrc
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    217400-plasmid-map-sequence-id-434131

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  47. His-DERA

    Plasmid
    #203225
    Purpose
    For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Article
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    deoxyribose aldolase
    Use
    Tags
    N-terminal TEV cleavable 6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203225-plasmid-map-sequence-id-400074

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  50. pPT-PCR C2

    Plasmid
    #141247
    Purpose
    PCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 2
    Depositor
    Bogumil Karas
    Article
    Cochrane et al Algal Research, 2020. 50, 102014
    Insert
    Mitochondrial Genome of Phaeodactylum tricornutum
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Repeat region removed, additional minor mutations…
    Promoter
    Available since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    141247-plasmid-map-sequence-id-377468

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Showing: 301 - 350 of 1192 results