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We narrowed to 4,045 results for: golden gate

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Showing: 301 - 350 of 4045 results

  2. pOGG014

    Plasmid
    #113988
    Purpose
    Vector construction module. Endlinker/terminator module to circularize plasmid following Part 4
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Endlinker/terminator Part 4
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113988-plasmid-map-sequence-id-223089

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  3. pOGG015

    Plasmid
    #113989
    Purpose
    Vector construction module. Endlinker/terminator module to circularize plasmid following Part 5
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Endlinker/terminator Part 5
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113989-plasmid-map-sequence-id-223087

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  4. pOGG013

    Plasmid
    #113987
    Purpose
    Vector construction module. Endlinker/terminator module to circularize plasmid following Part 3
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Endlinker/terminator Part 3
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113987-plasmid-map-sequence-id-223414

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  5. pOGG016

    Plasmid
    #113990
    Purpose
    Vector construction module. Endlinker/terminator module to circularize plasmid following Part 6
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Endlinker/terminator Part 6
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    113990-plasmid-map-sequence-id-223084

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  6. pOGG203

    Plasmid
    #115504
    Purpose
    Clone of a level 1 unit which encodes a constitutively-expressed mCherry
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    pL1M-F2-pNeo pOGG001, mCherry EC15071, T-pharma(pOGG003
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    115504-plasmid-map-sequence-id-223070

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  7. pL1P6 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198049
    Purpose
    MoClo Golden Gate Level 1 Position 6. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198049-plasmid-map-sequence-id-391867

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  8. pLM433

    Plasmid
    #217293
    Purpose
    For creating CyanoTag vectors by Golden Gate cloning (BspQI version)
    Depositor
    Luke Mackinder
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    mNeonGreen - 3x FLAG
    Available Since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217293-plasmid-map-sequence-id-435614

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  9. PACE_SP_SD4_AraC_S170_LOV

    Plasmid
    #242144
    Purpose
    Contains an AraC-LOV Chimera gene to assemble a selection phage missing gene III with SapI Golden Gate Assembly
    Depositor
    Dominik Niopek
    Insert
    AraC S170 LOV
    Expression
    Bacterial
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    242144-plasmid-map-sequence-id-484654

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  10. pMC-Dest-BsaI

    Plasmid
    #200550
    Purpose
    Mini circle destination vector with BsaI sites for Golden Gate Assembly
    Depositor
    Junsu Kang
    Type
    Empty backbone
    Use
    Minicircle cloning vector
    Available Since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    200550-plasmid-map-sequence-id-482399

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  11. PACE_SP_SD4_AraC_R5_S170_LOV

    Plasmid
    #242146
    Purpose
    Contains an AraC R5 Variant-LOV Chimera gene to assemble a selection phage missing gene III with SapI Golden Gate Assembly
    Depositor
    Dominik Niopek
    Insert
    AraC R5 S170 LOV
    Expression
    Bacterial
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    242146-plasmid-map-sequence-id-484661

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  12. PACE_SP_SD4_AraC_R2_S170_LOV

    Plasmid
    #242145
    Purpose
    Contains an AraC R2 Variant-LOV Chimera gene to assemble a selection phage missing gene III with SapI Golden Gate Assembly
    Depositor
    Dominik Niopek
    Insert
    AraC R2 S170 LOV
    Expression
    Bacterial
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    242145-plasmid-map-sequence-id-484655

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  13. L0I80_Bxb1mut(noBsaI)_GG1-GG4

    Plasmid
    #219715
    Purpose
    Level 0 Bxb1 integrase with mutated BsaI sites, GG1 and GG4 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG1-Bxb1(noBsaI0)-GG4
    Use
    Synthetic Biology
    Available Since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219715-plasmid-map-sequence-id-435740

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  15. pOPS0379

    Plasmid
    #115510
    Purpose
    Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing sfGFP
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Rlv3841pNifH(pOGG082) sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115510-plasmid-map-sequence-id-224287

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  16. pOPS0254

    Plasmid
    #115506
    Purpose
    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing celB
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Rlv3841pNifH pOGG082, celB pOGG050, T-pharma pOGG003 assembled in pOGG026
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115506-plasmid-map-sequence-id-224294

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  17. pOPS0377

    Plasmid
    #115509
    Purpose
    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing sfGFP
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Constitutive promoter pNeo pOGG001
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115509-plasmid-map-sequence-id-224290

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  18. pOPS0750

    Plasmid
    #115511
    Purpose
    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing gusA.
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Constitutive promoter pNeo pOGG001
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    115511-plasmid-map-sequence-id-223075

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  19. pOPS0314

    Plasmid
    #115507
    Purpose
    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celB
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    celB pOGG050, T-pharma pOGG003 assembled in pOGG026
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Constitutive promoter pNeo pOGG001
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115507-plasmid-map-sequence-id-224284

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  20. pOPS0253

    Plasmid
    #115505
    Purpose
    Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing gusA.
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Rlv3841pNifH pOGG082, gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115505-plasmid-map-sequence-id-224288

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  24. pGGG-TaBSS2-tdTom

    Plasmid
    #242018
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of transgenic tdTomato driven by the Basal Spikelet Specific 2 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    tdTomato
    Tags
    NLS
    Expression
    Plant
    Promoter
    BSS2
    Available Since
    Nov. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    242018-plasmid-map-sequence-id-492286

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  25. pGGG-TaBSS1-tdTom

    Plasmid
    #242014
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of transgenic tdTomato driven by the Basal Spikelet Specific 1 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    tdTomato
    Tags
    NLS
    Expression
    Plant
    Promoter
    BSS1
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    242014-plasmid-map-sequence-id-487879

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  26. pXZ13lacZalphaKanR

    Plasmid
    #160230
    Purpose
    Like pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.
    Depositor
    Ian Duncan
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    lac
    Available Since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    160230-plasmid-map-sequence-id-314765

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  27. pDM028

    Plasmid
    #216809
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and hph selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216809-plasmid-map-sequence-id-430135

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  28. pDM068

    Plasmid
    #216811
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216811-plasmid-map-sequence-id-430142

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  29. pDM026

    Plasmid
    #216808
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216808-plasmid-map-sequence-id-429385

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  34. pDM030

    Plasmid
    #216810
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and ble selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216810-plasmid-map-sequence-id-430137

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  40. PGR-Blue

    Plasmid
    #68374
    Purpose
    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.
    Depositor
    Nathan Reyna
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    68374-plasmid-map-sequence-id-121945

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  41. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Article
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

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  43. pNDGG005

    Plasmid
    #231309
    Purpose
    Vector part for BEVA; BEVA2.0 Position 3 RSF1010 broad host range origin of replication with oriT, CmR.
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 Position 3 RSF1010 broad host range origin of replication with oriT, CmR.
    Expression
    Bacterial
    Available Since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    231309-plasmid-map-sequence-id-472146

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  44. pNDGG006

    Plasmid
    #231310
    Purpose
    Vector part for BEVA; BEVA2.0 Position 3 p15A narrow host range origin of replication with oriT, CmR.
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 Position 3 p15A narrow host range origin of replication with oriT, CmR.
    Expression
    Bacterial
    Available Since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231310-plasmid-map-sequence-id-463781

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  45. pNDGG007

    Plasmid
    #231311
    Purpose
    Vector part for BEVA; BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.
    Expression
    Bacterial
    Available Since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231311-plasmid-map-sequence-id-463780

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Showing: 301 - 350 of 4045 results