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Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II

Plasmid

#52093

Purpose

Retroviral expression of all four WT CD3 subunits, co-expressed with mAmetrine

Depositor

Dario Vignali

Article

Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

Inserts

CD3 delta (Cd3d Mouse)
CDC gamma (Cd3g Mouse)
CD3 epsilon (Cd3e Mouse)
CD3 zeta (Cd247 Mouse)

Use

Retroviral

Tags

F2A, IRES-EGFP, P2A, and T2A

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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Human Myc-XIAP

Plasmid

#217627

Purpose

Mammalian expression of Myc tagged XIAP

Depositor

Ethan Lee

Article

Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

Insert

XIAP (XIAP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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MO70-N-Flag-hCRACR2A-isoform-a

Plasmid

#79592

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
p-mCherry-C1-hCRACR2A-isoform-a

Plasmid

#79593

Purpose

Transient expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
pLV_PGK_LTB4R(T308A)

Plasmid

#208088

Purpose

Mammalian expression of the human LTB4 receptor LTB4R with the T308A mutation.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

LTB4R(T308A) (LTB4R Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

T308A

Promoter

PGK

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_PGK_LTB4R(S310A)

Plasmid

#208089

Purpose

Mammalian expression of the human LTB4 receptor LTB4R with the S310A mutation.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

LTB4R(S310A) (LTB4R Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

S310A

Promoter

PGK

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_PGK_LTB4R(T308A,S310A)

Plasmid

#208090

Purpose

Mammalian expression of the human LTB4 receptor LTB4R with the T308A and S310A mutations.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

LTB4R(T308A,S310A) (LTB4R Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

T308A, S310A

Promoter

PGK

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_PGK_3xHA-LTB4R

Plasmid

#208091

Purpose

Mammalian expression of the wild-type human LTB4 receptor LTB4R with an N-terminal 3x HA tag.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

3xHA-LTB4R (LTB4R Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

none

Promoter

PGK

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pPICZ-pre-Ost1-pro-alphaf-Btl2

Plasmid

#117665

Purpose

Study secretion efficiency of Btl2

Depositor

Benjamin Glick, Pau Ferrer

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-Ost1-pro-alphaf-Btl2

Use

Tags

Expression

Yeast

Mutation

This plasmid encodes the lipase Btl2 together wit…

Promoter

pAOX1

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2_BirA_NRF2

Plasmid

#136524

Purpose

Used as a donor vector to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

NRF2 (NFE2L2 Human)

Use

Entry vector for gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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CROPseq-Guide-Puro-mCherry2

Plasmid

#219679

Purpose

CROPseq vector based on #86708 with an additional mCherry2 fluorescent gene

Depositor

Christoph Bock

Article

Boccuni et al Sci Signal. 2022 Dec 13;15(764):eabq5389

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pSLIK_hygro_BirA_NRF2

Plasmid

#136539

Purpose

Lentiviral expression vector for an inducible BirA-NRF2

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

BirA-NRF2 (NFE2L2 Human)

Use

Lentiviral; Destination vector for gateway cloning

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2020

Availability

Academic Institutions and Nonprofits only

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pBABE_hygro_mRFP1_NRF2

Plasmid

#136579

Purpose

mammalian expression of mRFP1-tagged human NRF2

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

NRF2 (NFE2L2 Human)

Use

Retroviral

Tags

N-mRFP1-FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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tet_pLKO.1_puro_shNRF2 #1

Plasmid

#136584

Purpose

Expresses an inducible short hairpin targeting human NRF2 sequence

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

shNRF2v1 (NFE2L2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2020

Availability

Academic Institutions and Nonprofits only

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tet_pLKO.1_puro_shNRF2 #2

Plasmid

#136585

Purpose

Expresses an inducible short hairpin targeting human NRF2 sequence

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

shNRF2v2 (NFE2L2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-Rheb (Q64L)-pcw107

Plasmid

#64607

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Q64L

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
myr-FLAG-PIK3CA-pcw107

Plasmid

#64605

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

PIK3CA (PIK3CA Bovine)

Use

Lentiviral

Tags

FLAG and myristoylated

Expression

Mutation

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
FLAG-IKKbeta (S177E,S181E)-pcw107

Plasmid

#64609

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

IKBKB

Use

Lentiviral

Tags

FLAG

Expression

Mutation

S177E, S181E

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
6xHis-MBP-TEV-mDvl2PDZ(264-353)_pCDFduet

Plasmid

#216386

Purpose

Bacterial expression vector for MBP-tagged mouse Dvl2 PDZ domain (residues 264-353)

Depositor

William Weis

Article

Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

Insert

Dishevelled-2 (Dvl2 Mouse)

Use

Tags

6xHis-MBP-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-TEV-mDvl2DEP(416-510)_pCDFduet

Plasmid

#216387

Purpose

Bacterial expression vector for MBP-tagged mouse Dvl2 DEP domain (residues 416-510)

Depositor

William Weis

Article

Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

Insert

Dishevelled-2 (Dvl2 Mouse)

Use

Tags

6xHis-MBP-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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