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pQE80L TriCatcher-RGDSP

Plasmid

#112631

Purpose

Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcher

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

TriCatcher-RGDSP

Use

Tags

His6 and TEV cleavage site

Expression

Bacterial

Mutation

central RGDSP integrin-binding sequence

Promoter

T5

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3-hTICAM1-VenusFlag

Plasmid

#197632

Purpose

Human TRIF protein expression in mammalian cells

Depositor

Haian Fu

Insert

TICAM1 (TICAM1 Human)

Use

Tags

Venus-Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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ICAM4-CD4d3+4-bio

Plasmid

#73102

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding ICAM4 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

ICAM4 (ICAM4 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pFS_0143-pET30-Fusicatenibacter_saccharivorans

Plasmid

#116952

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with classic acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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eGFP-Silicatein-pET28

Plasmid

#198012

Purpose

C-terminal eGFP fusion to truncated silicatein insert with N- and C-terminal hexahistidine tag for purification

Depositor

Bryan Berger

Article

Vigil et al Materials Advances 2, 2023

Insert

eGFP-silicatein

Use

Tags

hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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ciCas9(L22)_pcDNA5

Plasmid

#100551

Purpose

Expresses ciCas9(L22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

ciCas9(L22)

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pFS_0160-pET30-Fusicatenibacter_saccharivorans_ARRAY2

Plasmid

#116953

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with classic acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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ciCas9(F22)_pcDNA5

Plasmid

#100552

Purpose

Expresses ciCas9(F22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

ciCas9(F22)

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-VenusFlag-hTICAM1

Plasmid

#197631

Purpose

Human TRIF protein expression in mammalian cells

Depositor

Haian Fu

Insert

TICAM1 (TICAM1 Human)

Use

Tags

Venus-Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST26-hTICAM1-Nluc

Plasmid

#197634

Purpose

Human TRIF protein expression in mammalian cells

Depositor

Haian Fu

Insert

TICAM1 (TICAM1 Human)

Use

Tags

NanoLuciferase

Expression

Mammalian

Mutation

Promoter

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-M.Mus.1

Plasmid

#196681

Purpose

Rep/Cap plasmid for the production of M.Mus.1, an AAV capsid with CNS tropism in mice.

Depositor

Pardis Sabeti

Article

Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Tags

Expression

Mutation

WVLPSGG insert between amino acids 588 and 589 of…

Promoter

p41

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MDV1B

Plasmid

#196680

Purpose

Rep/Cap plasmid for the production of MDV1B, an AAV capsid with CNS tropism in mice.

Depositor

Pardis Sabeti

Article

Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Tags

Expression

Mutation

KTVGTVY insert between amino acids 588 and 589 of…

Promoter

p41

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-PAL2

Plasmid

#196691

Purpose

Rep/Cap plasmid for the production of PAL2, an AAV capsid with CNS tropism in macaques.

Depositor

Pardis Sabeti

Article

Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Tags

Expression

Mutation

PTQGTVR insert between amino acids 588 and 589 of…

Promoter

p41

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmBicaudalC_P

Plasmid

#147163

Purpose

Insect Expression of DmBicaudalC

Depositor

Elisa Izaurralde

Insert

DmBicaudalC (BicC Fly)

Use

Tags

Expression

Insect

Mutation

12 silent and three non silent mutattions I331T, …

Promoter

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pNUT N6His mICA NG

Plasmid

#70162

Purpose

Expresses N-His tagged nonglycosylated mouse inhibitor of carbonic anhydrase

Depositor

Ross MacGillivray, Anne Mason

Article

Wang et al Biochem J. 2007 Aug 15;406(1):85-95.

Insert

mouse inhibitor of carbonic anhydrase

Use

Tags

N-terminal signal peptide, 4 aa link, 6 His, Fact…

Expression

Mammalian

Mutation

Asn470Asp and Asn645Asp

Promoter

SV40

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pET28b-DiCas7-11-His

Plasmid

#234482

Purpose

Plasmid for bacterial expression and purification of DiCas7-11 protein (human codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Article

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

DiCas7-11

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pColdI-SBP-DDX3_helicase_core

Plasmid

#233622

Purpose

Expression of SBP-tagged helicase core region of DDX3X in E.coli

Depositor

Shintaro Iwasaki

Article

Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

Insert

SBP-DDX3X helicase core (EIF4A1 Human)

Use

Tags

His6-SBP

Expression

Bacterial

Mutation

Promoter

Available since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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MPS1 Candida albicans

Plasmid

#232146

Purpose

MPS1 from Candida albicans codon optimized for S. cerevisiae in pUCIDT

Depositor

Mark Winey

Article

Fabritius et al PLoS One. 2024 Mar 26;19(3):e0301084. do

Insert

MPS1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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iCAP-BI151-KanR

Plasmid

#209523

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Eid et al Nat Commun. 2024 Aug 4;15(1):6602. doi:

Insert

AAV-BI151 Cap

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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iCAP-BI152-KanR

Plasmid

#209524

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Eid et al Nat Commun. 2024 Aug 4;15(1):6602. doi:

Insert

AAV-BI152 Cap

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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