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Plasmid#184287PurposeAnti-mouse IgG1 Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbMmIgG1-Tn5
UseTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRL278
Plasmid#70256PurposesacB plasmid with polylinker; kanamycin/neomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.DepositorTypeEmpty backboneUseConditionally lethalTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOlyA(WT)
Plasmid#132391PurposeOstreolysin A for sensing sphingomyelin/cholesterol complexesDepositorInsertOstreolysin A wild-type
UseTagsHis6 tagExpressionBacterialMutationMutations: C62S, C94S, S151CPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
NW9
Plasmid#133442PurposeCovalently circularized nanodisc NW9 for studying membrane proteins and viral entry.DepositorInsertNW9
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pre_II_CS3_9072_v1
Plasmid#126854Purpose9072 b scaffold from compatibility group 3, made by method IIDepositorInsertII_CS3_9072_v1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbMmKappa-Tn5
Plasmid#184286PurposeAnti-mouse kappa chain specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbMmKappa-Tn5
UseTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST-KiT
Plasmid#44562Purposefor integrative inducible expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbMmIgG2a-Tn5
Plasmid#184288PurposeAnti-mouse IgG2a Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbMmIgG2a-Tn5
UseTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB1620 hU6-BpiI-Cas13bt3-DR
Plasmid#176315PurposehU6-BpiI-Cas13bt3-DR (crRNA expression)DepositorInsertCas13bt3 crRNA direct repeat
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pALOD4
Plasmid#111026PurposeExpresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472ADepositorInsertDomain 4 of Anthrolysin O
UseTagsHis6 tagExpressionBacterialMutationMutations: S404C, C472APromoterT7Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
NW11
Plasmid#133443PurposeCovalently circularized nanodisc NW11 for studying membrane proteins and viral entry.DepositorInsertNW11
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pST-KT
Plasmid#44561Purposefor ectopic inducible expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialMutationPromoterUV15 promoterAvailable sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pST-K
Plasmid#44560Purposefor ectopic constitutive expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialMutationPromoterUV15 promoterAvailable sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
bRA89
Plasmid#100950PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
NW30
Plasmid#133444PurposeCovalently circularized nanodisc NW30 for studying membrane proteins and viral entry.DepositorInsertNW30
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPR220.08
Plasmid#78553PurposePlasmid constitutively expressing phycocyanobilin (PCB) biosynthesis genes and UirS sensor kinase geneDepositorInsertuirS
UseSynthetic BiologyTagsExpressionMutationPromoterJ23108Available sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSP1E1D1
Plasmid#161605PurposeBacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formationDepositorInsertMSP1E1D1
UseTags7-HisExpressionBacterialMutation"extended" MSP1D1; contains repeat of h…PromoterT7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
LgBit-NbALFA
Plasmid#201484PurposeNanoBit detection of ALFA-tagged proteinDepositorInsertLgBit-NbALFA
UseTagsLgBitExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
NW50
Plasmid#133445PurposeCovalently circularized nanodisc NW50 for studying membrane proteins and viral entry.DepositorInsertNW50
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNOC-stacked-GFP-aequorin
Plasmid#101011PurposeConstruct for expression of a calcium sensitive luciferase reporter, GFP-aequorin, and hygromycin resistance gene by a bidirectional promoterDepositorTypeEmpty backboneUseAlgae, nannochloropsisTagsExpressionMutationPromoterRibiAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only