We narrowed to 2,299 results for: CO
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Plasmid#74944PurposeDosage control and tracing of reprogramming episomesDepositorAvailable SinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only
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pF1-Hs Cdk1/cyclin B1
Plasmid#177330PurposeCo-expression of the human active Cdk1/cyclin B1 kinase complexDepositorTags2 x Strep-tag and 8 x HisExpressionInsectAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b/bPanK
Plasmid#73619PurposeProduces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)DepositorInsertpantothenate kinase (coaA Escherichia coli)
TagsHis6ExpressionBacterialMutationrelative to MG1655 sequence, changes glutamate-5 …PromoterT7Available SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
DYR1A_HUMAN_D0
Plasmid#79690PurposeThis plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-zeo_3xHA-TurboID-NLS
Plasmid#200022PurposeFor lentiviral transduction of 3xHA-TurboID-NLS under control of a doxycyline-inducible promoterDepositorInsert3xHA-TurboID-NLS
UseLentiviralExpressionMammalianPromoterTRE3GsAvailable SinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRIGF-IKZF1-V2
Plasmid#69045Purposeexpression of IKZF1 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.DepositorInsertIKZF1 (IKZF1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationsplice variant 2PromoterIRESAvailable SinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJPF0117
Plasmid#130267PurposeLentiviral vector for the co-expression of cytoplasmic bPAC and nuclear tagBFPDepositorInsertbPAC
UseLentiviralTagstagBFPExpressionMammalianPromoterhPGKAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-Ef1a-Puro-moxGFP
Plasmid#227273PurposeAAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)DepositorInsertAAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMG024 MBP-GSK3β-HA-His, GST_λPPase
Plasmid#196182PurposeCo-expresses MBP-GSK3β-HA-His (human GSK3β as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β-HA-HisDepositorAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL2-mGold-P2A-EBFP2
Plasmid#157998PurposeCo-expression of mGold and EBFP2 in mammalian cells.DepositorInsertmGold-P2A-EBFP2
ExpressionMammalianMutationmGold is mVenus with L46F;T63S mutationsPromoterCMVAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIRIGF-IKZF3
Plasmid#69046Purposeexpression of IKZF3 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.DepositorAvailable SinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-dCasRx-RBM25-IRES-BleoR
Plasmid#221002PurposeStable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.DepositorInsertdCasRx-RBM25-IRES-BleoR
UseCRISPRTagsNLS and NLS-HAExpressionMammalianPromoterCAGAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU (V5 and HA)
Plasmid#222486PurposeCo-expression of two genes in yeast with V5 and HA tags and LEU2 selectionDepositorTypeEmpty backboneTagsHA and V5ExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-HIS (V5 and HA)
Plasmid#222487PurposeCo-expression of two genes in yeast with V5 and HA tags and HIS3 selectionDepositorTypeEmpty backboneTagsHA and V5ExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBI/Rabaptin-5
Plasmid#171851Purposeinducible expression of Rabaptin-5 in mammalian cells via co-transfection with pTet-Off.DepositorInsertRabaptin-5 (human)
ExpressionMammalianAvailable SinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDC183
Plasmid#106415PurposeCo-expresses trans SNARE complex with pQZ210 in E. coliDepositorInsertsExpressionBacterialMutationFragment 141-204, Codon optimization and Fragment…PromoterT7Available SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
p184_LTJ_sgRNACD90.2
Plasmid#82670PurposesgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.DepositorInsertsgRNA targeting mouse CD90.2
UseCRISPRExpressionMammalianPromoterU6Available SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-auxilin(547-910)
Plasmid#62572PurposeCodes for a GST-auxilin fusion protein that is functional as a co-factor in hsc70-dependent clathrin coat disruptionDepositorInsertAuxilin
TagsGSTExpressionBacterialAvailable SinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-Ef1a-Puro-mScarlet
Plasmid#227274PurposeAAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)DepositorInsertAAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVH-EF1a-GFP-P2A-KLF4
Plasmid#130694PurposeLentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.DepositorInserthuman KLF4 (KLF4 Human)
UseLentiviralAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only