We narrowed to 1,621 results for: nif

1. CoV2-N-R203M

   Plasmid

   #177952

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203M). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (R203M) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R203M

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJRH051

   Plasmid

   #171625

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-BsmBI-Destination
   mNeon
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a and U6

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-UBC-hAAT-IRES-ZsGreen-W

   Plasmid

   #126702

   Purpose

   lentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreen

   Depositor

   Darrell Kotton

   Article

   Wilson et al Am J Respir Cell Mol Biol. 2008 Aug . 39

   Insert

   Alpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   UBC

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. CoV2-N-WT-Hu1

   Plasmid

   #177937

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Klf2-t2A-mCherry

   Plasmid

   #127539

   Purpose

   Expresses mouse Klf2 and mCherry via t2A linker.

   Depositor

   Jennifer Mitchell

   Article

   Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

   Insert

   Klf2 (Klf2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

   Plasmid

   #101209

   Purpose

   Bacterial expression plasmid for SpCas9-HF1 variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant N497A/R661A/Q695A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A and Q926A

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCerulean-VSVG

   Plasmid

   #11913

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

   Insert

   VSVG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F64L, S65T, Y66W, S72A, N146I, Y145A, H148D, M15…

   Promoter

   Available since

   Sept. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CoV2-N-S202R

   Plasmid

   #177950

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S202R). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (S202R) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S202R

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJJGL001

   Plasmid

   #180605

   Purpose

   Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Insert

   DpbCasX
   

   Use

   CRISPR

   Tags

   10x His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CoV2-N-P199L

    Plasmid

    #177949

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P199L). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P199L) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P199L

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOPS0381

    Plasmid

    #133231

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET 2C-T10-dCasX

    Plasmid

    #126418

    Purpose

    E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    DpbCasX
    

    Use

    CRISPR

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOPS0382

    Plasmid

    #133232

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with constitutive promoter PsNifH (pOGG043), mCherry (EC15071) and T-pharma (pOGG003)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAT003

    Plasmid

    #171628

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-TRAC
    mNeon
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1-a

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJRH-1365 LV EF1a-CD28 IRES-EGFP

    Plasmid

    #201921

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD28

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pH3mCHSH2

    Plasmid

    #101053

    Purpose

    This is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.

    Depositor

    Jennifer Lodge

    Article

    Upadhya et al Fungal Genet Biol. 2017 Sep 1. pii: S108

    Insert

    Cryptococcus mCherry expression plasmid
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Cryptococcus Histone3 promoter

    Available since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pyc111s

    Plasmid

    #158781

    Purpose

    ribosome-tagged, Cre-dependent GCaMP for soma localization

    Depositor

    Zachary Knight, Jennifer Garrison

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    GCaMP6s-RPL10a
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CoV2-N-T205I

    Plasmid

    #177954

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (T205I). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (T205I) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T205I

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA

    Plasmid

    #81185

    Purpose

    Expresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. coli with TEV-cleavable N-terminal His-MBP tag on Cas5c

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Inserts

    Cas5c
    Cas8c
    Cas7
    

    Use

    CRISPR

    Tags

    6xHis - MBP - TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBLO 62.4

    Plasmid

    #123123

    Purpose

    Human expression vector for deltaproteobacteria CasX and sgRNA

    Depositor

    Benjamin Oakes, Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Inserts

    CasX protein
    CasX sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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