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Plasmid#153328PurposeTriggers secretion of SptP120 fused to the K27 DARPin from Salmonella upon induction with arabinoseDepositorInsertSptP120-K27 DARPin
UseSalmonella expressionTagsExpressionMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
spa-GCaMP6f
Plasmid#67558Purposephotoactivatable fluorescent calcium reporterDepositorInsertspa-GCaMP6f
UseTagsHIS-tagExpressionMammalianMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…PromoterCMVAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
GPD 104QDProGFP p416
Plasmid#15569DepositorInserthtt 104Q Delta Pro (HTT Human)
UseTagsGFPExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLN410 (FuGW-S(E2F1)p-ECFP sponge control)
Plasmid#105182PurposeModule 2 control - synthetic promoter E2F1 drives ECFP only (no miR1 binding sites)DepositorInsertS(E2F1)p-ECFP (Cerulean) sponge control
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Lyn-Y-GECO1
Plasmid#58200PurposeExpress the membrane targeted Y-GECO1 in neuronsDepositorInsertY-GECO1.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1 Insulator ObLiGaRe/EPB71
Plasmid#90018PurposeDonor vector for ObLiGaRe/ZFN mediated targeting to AAVS1 locusDepositorInsertMCS flanked by insulator and inverted ZFN binding sites
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS406 ATG1-ATG17-3'UTR
Plasmid#166848PurposeExpresses Atg1 with ATG17 3'UTR in yeasts.DepositorInsertATG1 (ATG1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterATG1 PromoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-YAP-WW1-RRK
Plasmid#104306Purposebacterial expression of RKK mutant WW domain 1 of YAP; RRK mutant binds to PPxY where Y is phosphorylatedDepositorInsertYAP WW1 domain (YAP1 Human)
UseTagsGSTExpressionBacterialMutationWW domain 1 RRK mutant (TTAAATCACATCGATCAG to AGA…PromotertacAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
p5B3-T
Plasmid#92021PurposeExpresses TALE targeted to lysA promoter with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lysA with N-terminal TEV protease cleavage site
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…ExpressionMutationS219VPromoterAvailable sinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
GAL FLAG72QDProCFPp303
Plasmid#15586DepositorInserthtt 72Q Delta Pro (HTT Human)
UseTagsCFP and FLAGExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-GEX-GECO1
Plasmid#32464DepositorInsertGEX-GECO1.0
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…PromoterAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
Venus-Cdc20 R132A (1768)
Plasmid#26059DepositorInsertCdc20 (CDC20 Human)
UseTagsVenusExpressionMammalianMutationMutation in the Mad2 binding site, R132APromoterAvailable sinceAug. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFvl160
Plasmid#64753PurposeRecombination-Induced Tag Exchange (RITE) Cassette, 3xHA to a 3xT7 epitope tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassetteTagsExpressionMutationPromoternoneAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-p107 1*2*
Plasmid#32387DepositorInsertp107 promoter (Rbl1 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutation1st and 2nd E2F binding sites mutated: GCG-->A…PromoterAvailable sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
MET FLAG103QDProCFPp303
Plasmid#15591DepositorInserthtt 103Q Delta Pro (HTT Human)
UseTagsCFP and FLAGExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
TB:2xP53_RE:MiniP
Plasmid#133881Purpose2 copies of the p53 DNA binding motifDepositorInsert2 copies of the p53 DNA binding motif
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156)
Plasmid#61939PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (1-156) in E.coliDepositorInsertsUseTags6x HISExpressionBacterialMutationConstruct ends at residue 156PromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL T157I-pBabe-puro
Plasmid#19240DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Threonin 157 to IsoleucinPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL A149T-pBabe-puro
Plasmid#19242DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Alanin 149 to ThreoninPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
HOXA9 (murine) FLAG pET
Plasmid#8494DepositorInsertHOXA9 (Hoxa9 Mouse)
UseTagsFLAG and HisExpressionBacterialMutationFLAG epitope tag replaces T7 tag in the pET back…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pU6Rosa-CAG-Gal4-Rad18UBD
Plasmid#119012PurposeMammalian expression of Rosa26 sgRNA, fusion protein of yeast Gal4 with human Rad18 Ubiquitin binding domain and BFP expressionDepositorInsertGal4 (GAL4 Budding Yeast)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLN481 (FuGW-G8p-IL12-1Pe)
Plasmid#105191PurposeModule 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 transcripts containing miR1-Bs(Pe)DepositorInsertG8p-IL12-1Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
GAL 25Q+ProCFPp303
Plasmid#15574DepositorInserthtt25Q + Pro (HTT Human)
UseTagsCFPExpressionYeastMutationProline-rich region presentPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His6-OAZ-GSY-SPM-Ctag
Plasmid#163613PurposeExpresses truncated human Ornithine Decarboxylase Antizyme fused to SPM in bacterial cells for calcium-induced covalent coupling to Ornithine Decarboxylase.DepositorInsertOAZ-GSY-SPM
UseTagsC-tag and His6ExpressionBacterialMutationPromoterT7Available sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCASP-SptP120-NS1-HilA
Plasmid#153327PurposeTriggers secretion of SptP120 fused to the NS1 monobody from Salmonella upon induction with arabinoseDepositorInsertSptP120-NS1 Monobody
UseSalmonella expressionTagsExpressionMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
416-Met25 Kar2 printor HDEL
Plasmid#32129DepositorInsertPrintor (KLHL14 Human)
UseTagsHDEL and Kar2ExpressionYeastMutationPromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
GAL HA25QDProCFPp303
Plasmid#15578DepositorInserthtt 25Q Delta Pro (HTT Human)
UseTagsCFP and HAExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
GAL HA103QDProCFPp303
Plasmid#15579DepositorInserthtt 103Q Delta Pro (HTT Human)
UseTagsCFP and HAExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEF Itk K390R myc
Plasmid#20707DepositorInsertItk K390R (Itk Mouse)
UseTagsmycExpressionMammalianMutationKinase Dead. K390R.PromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCALNL_chr13_102010574-102010650
Plasmid#81213PurposepCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 13 (numbers in common name refers to pos. in hg38)DepositorInsertgix-Neo-gix deletion cassette upstream of EGFP
UseTagsExpressionMammalianMutationPromoterpCBaAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCALNL_Ch12_62418577-62418652
Plasmid#81212PurposepCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 12 (numbers in common name refer to pos. in hg38)DepositorInsertgix-Neo-gix deletion cassette upstream of EGFP
UseTagsExpressionMammalianMutationPromoterpCBaAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAP2050-2
Plasmid#105243Purposemammalian GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linkerDepositorInsertGFP11 with extra-sequence (GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKV005
Plasmid#64763PurposeRecombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, invariable Myc tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassetteTagsExpressionMutationPromoternoneAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
HM3C NaVSp1_p pET24b
Plasmid#127852PurposeBacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.DepositorInsertNaVSp1
UseTags3C Cleavage site, His Tag, and MBPExpressionBacterialMutationPromoterAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5A1-Ti
Plasmid#92018PurposeExpresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible catalytically inactive TEV proteaseDepositorInsertsTALE targeting lac operator with 3 TEV cleavage sites
Catalytically Inactive TEV Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…ExpressionMutationS219V, C151APromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-8A/S55D
Plasmid#114032PurposeInducible expression of eGFP-tagged HEC1-8A/S55D in FLPin Trex cell linesDepositorInserteGFP-HEC1-8A/S55D (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationHec1 - 8A/S55DPromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAP2042-1
Plasmid#105244Purposemammalian eGFP with TEV::3xFlag::Ollas::Myc::V5::HAtag::other linkerDepositorInserteGFP with extra-sequence (TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker::eGFP)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_FLAGHA_PRKRA_sgRNA_PAM_mutant
Plasmid#106112PurposeInducible gene expression of FlagHA-PRKRA sgRNA PAM mutantDepositorInsertPRKRA (PRKRA Human)
UseTagsFlag and HAExpressionMammalianMutationsilent mutation of Gly32 (GGG-->GGT)PromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
p236 pPr-CREM
Plasmid#8404PurposeExpression of Cre from tissue-specific, androgen-inducible probasin promoter, with chimeric/β-globin intron within the Cre coding sequence.DepositorInsertprobasin driven CREM
UseCre/LoxTagsExpressionMammalianMutationmodified CrePromoterProbasin promoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLN491 (FuGW-G8p-CCL21-1Pe)
Plasmid#105193PurposeModule 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of CCL21 transcripts containing miR1-Bs(Pe)DepositorInsertG8p-CCL21-1Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA mouse NHERF-1 (1-325)
Plasmid#32706DepositorInsertsolute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (Nherf1 Mouse)
UseTagsnoneExpressionMammalianMutationDeleted last 30 AA putative Ezrin binding sitePromoterCMVAvailable sinceFeb. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mPar6a WT
Plasmid#86148PurposeFLAG tagged mouse Par6 wild type - Binds to aPKC, Par3, Lgl, Cdc42DepositorInsertPar6 (Pard6a Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterLTRAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP N184X with 5'UTR
Plasmid#92375PurposeInducible expression of N184X spastin M1 and M87 in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutationS184XPromoterTet-responsive P tightAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKV006
Plasmid#64764PurposeRecombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, invariable V5 tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassetteTagsExpressionMutationPromoternoneAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5A7-T
Plasmid#92020PurposeExpresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lac operator with N and C-terminal TEV protease cleavage sites
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…ExpressionMutationS219VPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
p5A3-T
Plasmid#92019PurposeExpresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lac operator with N-terminal TEV cleavage site
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…ExpressionMutationS219VPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
Gal4-p67MBP
Plasmid#52DepositorInsertp67 myb binding protein (Mybbp1a Mouse)
UseTagsGal4DBDExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-His-VHL-Flag(117-213)
Plasmid#29501DepositorInsertVon Hippel-Lindau (VHL Human)
UseTagsFlag and HisExpressionInsectMutationVHL(117-213) onlyPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET151-hUCH37 (1-228)
Plasmid#61932PurposeExpression of hUCH37 (1-228) in E.coliDepositorInserthUCH37 (1-228) (UCHL5 Human)
UseTags6x HISExpressionBacterialMutationStop codon introduced after residue K228PromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only