We narrowed to 58,318 results for: tra

1. pUASTLOTattB_mCherry-CD8-vhhGFP

   Plasmid

   #163930

   Purpose

   Expression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancer

   Depositor

   Markus Affolter

   Article

   Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

   Insert

   Fusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
   

   Use

   Cre/Lox

   Tags

   mCherry

   Expression

   Insect

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX4

   Plasmid

   #183903

   Purpose

   donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

   Insert

   GFP
   

   Expression

   Insect

   Promoter

   Aedes aegypti PUb

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX3

   Plasmid

   #183904

   Purpose

   donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

   Insert

   GFP
   

   Expression

   Insect

   Promoter

   Aedes aegypti Polyubiquitin

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBCC091

   Plasmid

   #202218

   Purpose

   Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum

   Depositor

   Paul Schulze-Lefert

   Article

   Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

   Insert

   pBCC25, pBCC30, pBCC55, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
   

   Expression

   Bacterial

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-LS

   Plasmid

   #205438

   Purpose

   Expression of Gaussia luciferase (gLuc) and spectinomycin resistance (Spec) genes

   Depositor

   Masamichi Kamihira

   Article

   Huang et al J Biosci Bioeng. 2021 Nov;132(5):469-478

   Inserts

   Gaussia luciferase gene
   spectinomycin resistance gene
   

   Use

   Cre/Lox and Luciferase; Chlamydomonas expression

   Promoter

   Heat shock protein 70A (HSP70A)/ribulose bisphosp…

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKSB-AeU6-1gRNA

   Plasmid

   #183912

   Purpose

   gRNA-expressing plasmid under Aedes aegypti AAEL017702 U6 promoter

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

   Insert

   gRNA scaffold for protospacer cloning into BbsI sites
   

   Promoter

   Aedes aegypti U6 promoter (AAEL017702)

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKSB-AeU6-2gRNA

   Plasmid

   #183913

   Purpose

   gRNA-expressing plasmid under Aedres aegypti AAEL017774 U6 promoter

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

   Insert

   gRNA scaffold for protospacer cloning into BbsI sites
   

   Promoter

   Aedes aegypti U6 promoter (AAEL017774)

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFAB1907

   Plasmid

   #47857

   Purpose

   BIOFAB reporter plasmid for measuring BBa_B1006 U10 termination efficiency

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   BBa_B1006 U10
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Added two extra U at the end of Bba_1006 poly-U t…

   Promoter

   pLTetO12

   Available Since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-ATLASsnCre

   Plasmid

   #232351

   Purpose

   Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons, Cre payload. Must use with AAV8-DIO-Reporter viruses infected in postsynaptic cells.

   Depositor

   Don Arnold

   Article

   Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

   Has Service

   AAV5

   Insert

   ATLASsnCre
   

   Use

   AAV

   Tags

   ALFA-Tag

   Promoter

   hSyn

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3Luc-5XNF-kappaB

    Plasmid

    #185695

    Purpose

    Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

    Depositor

    Esther López-Bayghen

    Article

    Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

    Insert

    NF-kappaB (NFKB1 Human)
    

    Use

    Luciferase

    Promoter

    NF-kappaB binding site

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-TRE-C-prM-E-NS1

    Plasmid

    #175277

    Purpose

    AAV vector mediating inducible expression of 4 genes (the C-prM-E-NS1) of YFV-17D

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    C-prM-E-NS1 (POLY Yellow fever virus strain 17D)
    

    Use

    AAV

    Promoter

    tight TRE

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf

    Plasmid

    #130976

    Purpose

    Expression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

    Insert

    Hook3-SNAPf (HOOK3 Human)
    

    Tags

    8His-ZZ-LTLT and SNAPf

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-DIO-NS1

    Plasmid

    #175273

    Purpose

    AAV vector mediating Cre-depenent expression of NS1 gene of YFV-17D

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    Nonstructural protein 1 (NS1) of YFV-17D (POLY Yellow fever virus strain 17D)
    

    Use

    AAV

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. xlox dNGFR-TERT

    Plasmid

    #69805

    Purpose

    MLV retroviral vector expressing the hTERT reverse transcriptase immortalizing gene and a tNGFR marker for tracking

    Depositor

    David Ott

    Article

    Andersen et al AIDS Res Hum Retroviruses. 2007 Mar;23(3

    Insert

    hTERT reverse transcriptase (TERT Human)
    

    Use

    Retroviral

    Tags

    none

    Promoter

    MLV promoter

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-rBrokenheart

    Plasmid

    #203394

    Purpose

    AAV transfer plasmid containing a constitutive (non Cre-dependent) BrokenHeart construct: a piggyBac donor transposon interrupting tdTomato. Transposase activity rescues coding sequence.

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Yen et al Curr Protoc. 2023 Sep;3(9):e883. doi: 10

    Insert

    Brokenheart
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2 Notch1 LNG-6MT

    Plasmid

    #41738

    Depositor

    Raphael Kopan, Jeffrey Nye

    Article

    Kopan et al Proc Natl Acad Sci U S A. 1996 Feb 20;93

    Insert

    Notch-1 Lin-Notch-glp (LNG) repeat-containing construct (Notch1 Mouse)
    

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    Contains murine Notch-1 leader peptide (aa 1-23) …

    Available Since

    Jan. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pLKO-puro SNAP-CD86-eGFP-FKBP(f36v)

    Plasmid

    #200283

    Purpose

    Membrane protein fusion with extracellular SNAPtag and intracellular FKBP(f36v) dimerization domain and GFP reporter under Tetracycline-inducible promoter

    Depositor

    Kristina Ganzinger

    Article

    Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

    Insert

    SNAPtag-eGFP-CD86tm-eGFP-FKBP(f36v)
    

    Use

    Lentiviral

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF1010

    Plasmid

    #143742

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF547 (ZNF547 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-DIO-ATLASsnFlp

    Plasmid

    #232352

    Purpose

    Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons that express Cre, Flp payload. Must use with AAV8-fDIO-Reporter viruses infected in postsynaptic cells.

    Depositor

    Don Arnold

    Article

    Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

    Insert

    ATLASsnFlp
    

    Use

    AAV

    Tags

    ALFA-Tag

    Promoter

    hSyn

    Available Since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. MCP-MSN

    Plasmid

    #210704

    Purpose

    This plasmid express EF1a promoter driven MCP-MSN transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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