We narrowed to 5,868 results for: nls

1. XLone-Puro Cas13d P2A eGFP-NLS

   Plasmid

   #155182

   Purpose

   PiggybacTransposon-based tunable and temporal expression control of Cas13d and nuclear eGFP

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biomaterials. 2022 May 6;285:121569. doi

   Insert

   CasRx
   

   Use

   Unspecified; Piggybac transposon

   Tags

   NLS and P2A eGFP NLS

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PET-SpCas9-HF1-plus-NLS-6xHis

   Plasmid

   #207381

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   SpCas9-HF1-plus
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, Q695A, Q926A, amino acids 1005-1013 replac…

   Promoter

   T7

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. XLone-BSD NFIL3 P2A eGFP NLS

   Plasmid

   #140030

   Purpose

   Tunable and temporal expression control of NFIL3 and nuclear eGFP

   Depositor

   Xiaoping Bao

   Insert

   NFIL3 (NFIL3 Human, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-FLEX-tmeGFP-IRES-nls-mRuby2

   Plasmid

   #63060

   Purpose

   Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

   Depositor

   Bernardo Sabatini

   Article

   Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

   Insert

   nls-mRuby2
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-HOXD13(+9A)-IDR-mCherry-CRY2-NLS

   Plasmid

   #145263

   Purpose

   Mammalian Expression of Nuclear Opto HOXD13(+9A) IDR with SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXD13(+9A)-IDR (HOXD13 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A-repeat expanded

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. XE70 NLS Beta-catenin GFP in CS2+

   Plasmid

   #16838

   Depositor

   Randall Moon

   Article

   Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

   Insert

   NLS beta-catenin GFP (ctnnb1.L Frog)
   

   Use

   Xenopus expression

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

7. phage - ubc - nls - 2xmcp - mCherry - BirA*

   Plasmid

   #131136

   Purpose

   BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and mCherry; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

   Depositor

   Ralf Jansen

   Insert

   NLS-HA-2xMCP-mCherry-BirA*
   

   Use

   Lentiviral

   Tags

   NLS, HA

   Expression

   Mammalian

   Mutation

   BirA* = BIrA >R118G

   Promoter

   human ubiquitin C (UBC)

   Available since

   Aug. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)

   Plasmid

   #114090

   Purpose

   CAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized MbCas12a
   

   Use

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426)

   Plasmid

   #133788

   Purpose

   CAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized St3Cas9
   

   Use

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CAG

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAG-FNLS-T2A-EGFP-ires-puro

    Plasmid

    #121169

    Purpose

    CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    FNLS-T2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmyc-GFP-TNRC6A-NLS/NES-mut

    Plasmid

    #42002

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    R930A, R931A, R932A, R934A, F951A, F955A, I958A, …

    Promoter

    CMV

    Available since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409)

    Plasmid

    #133790

    Purpose

    CAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized NmeCas9
    

    Use

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CAG

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-eGFP-NLS-RNF168 delta RING

    Plasmid

    #133983

    Purpose

    mammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    deletion of RING; siRNA resistant sequence: GAGGA…

    Promoter

    CMV

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

    Plasmid

    #79889

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid

    #121171

    Purpose

    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    xFNLS-T2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA4/TO-myc NES-mCE NLS-Flag

    Plasmid

    #82468

    Purpose

    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    Myc-NES-mCE-Flag (Rngtt HIV, Mouse)
    

    Use

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-CMV-NLS-mTurquoise2-optoNES-WPRE

    Plasmid

    #159962

    Purpose

    optical regulation of nuclear export

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2-optoNES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    CMV-IE

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSP303 pRS306 pRPS2-Far1DS-NLS-mCherry

    Plasmid

    #68279

    Purpose

    SKARS: Fus3 MAPK activity sensor: Far1DS 2xNLS mCherry

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Far1DS NLS mCherry
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    RPS2

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pED73 pRS304 pRPS2-SteDS-NLS-mCitrine

    Plasmid

    #68278

    Purpose

    SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCitrine

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Ste7DS NLS mCitrine
    

    Use

    Tags

    mCitrine

    Expression

    Yeast

    Mutation

    Promoter

    pRPS2

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-4T3-ZO-2-1st-PDZ-delta-NLS

    Plasmid

    #27425

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Deletion of NLS-Nuclear Localization Signal: Nucl…

    Promoter

    Available since

    May 18, 2011

    Availability

    Academic Institutions and Nonprofits only