165,750 results

1. DD-Cas9 with filler sequence and Venus (EDCPV)

   Plasmid

   #90085

   Purpose

   This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be remove for cloning of desired sgRNA

   Depositor

   Raffaella Sordella

   Article

   Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Destabilized Domain and Flag

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTREtight2

   Plasmid

   #19407

   Depositor

   Markus Ralser

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Oct. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC-LYCipi

   Plasmid

   #53279

   Purpose

   Contains crtE, crtB, crtI, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces lycopene in Escherichia coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu

   Insert

   idi
   

   Use

   Low copy number bacterial cloning vector

   Promoter

   endogenous promoters

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

   Plasmid

   #139999

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

   Promoter

   CAG

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMOS003-lenti-CMV-GCaMPer

   Plasmid

   #65227

   Purpose

   ER calcium sensor for lentivirus production

   Depositor

   Adam Cohen, Brandon Harvey

   Article

   Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

   Insert

   GCaMPer
   

   Use

   Lentiviral

   Tags

   circularly permuted GFP

   Expression

   Mammalian

   Mutation

   GCaMP3 (D324G, D360G, D397G, D435G)

   Promoter

   CMV

   Available Since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQCXIP-GFP1-10

   Plasmid

   #68715

   Purpose

   Split GFP assay

   Depositor

   Yutaka Hata

   Article

   Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

   Insert

   GFP1-10
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Str-Golgin84_VSVG-SBP-EGFP

   Plasmid

   #65305

   Purpose

   synchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Golgin-84 (GOLGA5 Human)
   

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCerulean3-C1

   Plasmid

   #54605

   Purpose

   Localization: C1 Cloning Vector, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Type

   Empty backbone

   Tags

   mCerulean3

   Expression

   Mammalian

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUBC-popTag-QtE-mScarlet

   Plasmid

   #240101

   Purpose

   Mammalian lentiviral vector for expressing the mScarlet-tagged 50nm-GEMs, encoded by the encapsulin protein from Quasibacillus thermotolerans and fused to a PopZ tag.

   Depositor

   Liam Holt

   Article

   Xie et al Mol Cell. 2024 Jul 25;84(14):2698-2716.e

   Insert

   Encapsulin
   

   Use

   Lentiviral

   Tags

   mScarlet and popZ

   Expression

   Bacterial and Mammalian

   Promoter

   UBC promoter

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPROBE-NT

    Plasmid

    #37818

    Depositor

    Steven Lindow

    Article

    Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    Promotorless gfp reporter gene
    

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. Orai1 YFP

    Plasmid

    #19756

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    Orai1 (ORAI1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Oct. 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEVOL_PylRS(WT)-tRNA

    Plasmid

    #223512

    Purpose

    PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

    Depositor

    Edward Lemke

    Article

    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

    Inserts

    Pyrrolysyl-tRNA Synthetase (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    araBAD, rrnB, and glnS and proK

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST53-LRRK2-WT

    Plasmid

    #25044

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. BirA in pET28a (w400-2)

    Plasmid

    #26624

    Depositor

    Eric Campeau

    Insert

    BirA
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMZ827

    Plasmid

    #155321

    Purpose

    Constitutive expression of E-PIF6(1-100) in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-E-PIF6(1-100)-NLS-Tnos
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pROF051

    Plasmid

    #155328

    Purpose

    Constitutive expression of SRDX-EL222 in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-SRDX-NLS-EL222-Tnos
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA Flag Lamp2b-HA

    Plasmid

    #71292

    Purpose

    Flag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.

    Depositor

    Joshua Leonard

    Article

    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

    Insert

    Lamp2b (LAMP2 Human)
    

    Tags

    Flag, HA, and Lamp2 signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF1a_FOXA1_P2A_Hygro_Barcode

    Plasmid

    #120438

    Purpose

    Barcoded lentiviral vector to express FOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    FOXA1 (FOXA1 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMZ828

    Plasmid

    #155322

    Purpose

    Constitutive expression of PhyB(1-650)-VP16 in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-PhyB(1-650)-VP16-NLS-Tnos
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pROF021

    Plasmid

    #155326

    Purpose

    Expression of PULSE-controlled FLuc

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    (etr)8-(C120)5-PhCMVmin-FLuc-TSV40
    

    Use

    Luciferase and Synthetic Biology; Eukaryotic expr…

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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