We narrowed to 9,223 results for: Coli

1. pBAD33.lon

   Plasmid

   #22145

   Depositor

   Robert Sauer

   Article

   Gur et al Genes Dev. 2008 Aug 15. 22(16):2267-77.

   Insert

   Lon (lon E. coli K12)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None, WT gene

   Promoter

   Available since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKB224

   Plasmid

   #170014

   Purpose

   Encodes LacI-controlled expression of surface-displayed NC and PvirK-mNeonGreen reporter

   Depositor

   Jeffrey Tabor

   Article

   Brink et al bioRxiv

   Inserts

   mNeonGreen
   NC
   

   Use

   Synthetic Biology

   Tags

   Lpp, OmpA, and Tether

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac and PvirK

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEPkan-S2

   Plasmid

   #61601

   Purpose

   PCR template for en passant (two-step Red-mediated) mutagenesis

   Depositor

   Nikolaus Osterrieder

   Article

   Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

   Insert

   Kana_I-SceI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PRExpress

   Plasmid

   #122486

   Purpose

   A non-leaky heat-inducible gene expression system for Drosophila. (GenBank: MF058598.1)

   Depositor

   Renato Paro

   Article

   Akmammedov et al Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.

   Inserts

   LacZ
   poly-PRE
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG-mNaChBacMut-T2A-tdTomato

   Plasmid

   #60657

   Purpose

   Expresses mNaChBacMut-T2A-tdTomato

   Depositor

   Massimo Scanziani

   Article

   Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

   Insert

   mNaChBacMut-T2A-tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E191K

   Promoter

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEPkan-S

   Plasmid

   #41017

   Purpose

   PCR template for en passant (two-step Red-mediated) mutagenesis

   Depositor

   Nikolaus Osterrieder

   Article

   Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

   Insert

   Kana_I-SceI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKD‐SepRS-­EFSep-­5x tRNASep (B40 OTS)

   Plasmid

   #52054

   Purpose

   Used in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)

   Depositor

   Jesse Rinehart

   Inserts

   SepRS
   EF-Sep
   5x tRNA Sep
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

   Promoter

   Ptac

   Available since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTK204

   Plasmid

   #13534

   Depositor

   Wolf Frommer

   Article

   Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

   Insert

   FLIPW-CTYT
   

   Use

   Tags

   See map

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 27, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBSM

   Plasmid

   #67505

   Purpose

   Express the tRNAMet scaffold

   Depositor

   Luc Ponchon

   Article

   Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

   Insert

   modified tRNAMet
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lpp

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-K27 histone methylation reporter

    Plasmid

    #22865

    Depositor

    Alice Ting

    Article

    Lin et al J Am Chem Soc. 2004 May 19. 126(19):5982

    Insert

    K27 HMR
    

    Use

    Tags

    CFP and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

11. FLIPrbs

    Plasmid

    #17860

    Depositor

    Wolf Frommer

    Article

    Lager et al FEBS Lett. 2003 Oct 9. 553(1-2):85-9.

    Insert

    FLIPrbs
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    D208G

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFF753

    Plasmid

    #61453

    Purpose

    IPTG-inducible msd(wt)

    Depositor

    Timothy Lu

    Article

    Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

    Insert

    msd(wt)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pLacO

    Available since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNK141g2

    Plasmid

    #156380

    Purpose

    Expresses orthogonal RBS mPapaya reporter

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    mPapaya
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFF530

    Plasmid

    #61447

    Purpose

    IPTG-inducible msd_kanR(ON)

    Depositor

    Timothy Lu

    Article

    Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

    Insert

    msd_kanR(ON)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pLacO

    Available since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHG6

    Plasmid

    #188254

    Purpose

    Integrating plasmid contains the BT1 int/attP locus and the erythromycin-resistance gene for selection

    Depositor

    Hong Gao, Maggie Smith

    Article

    Gao et al Access Microbiol. 2021 Dec 8;3(12):00029

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    act1p/actII-orf4

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. DVC_p15A_FG

    Plasmid

    #217590

    Purpose

    Destination vector with chloramphenicol resistance and p15A origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.

    Depositor

    Wei Huang

    Article

    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMevB

    Plasmid

    #17819

    Depositor

    Jay Keasling

    Article

    Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

    Insert

    mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pFF745

    Plasmid

    #61450

    Purpose

    IPTG-inducible SCRIBE_kanR(ON)

    Depositor

    Timothy Lu

    Article

    Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

    Insert

    SCRIBE_kanR(ON)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pLacO

    Available since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIA030

    Plasmid

    #200424

    Purpose

    thrC::(Ppen-lacIΔ11-PAmCherry1 erm2). PALM integration vector includes PAmCherry1 fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.

    Depositor

    Rut Carballido-López

    Article

    Altinoglu et al bioRxiv 2023

    Insert

    LacI
    

    Use

    Tags

    PAmCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Ppen

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSCC2

    Plasmid

    #39993

    Depositor

    Paul Modrich

    Article

    Cheng et al J Biol Chem. 1984 Sep 25;259(18):11571-5

    Insert

    EcoRI endonuclease & EcoRI methylase
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    none

    Promoter

    n/a

    Available since

    Nov. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDrBB2

    Plasmid

    #18946

    Depositor

    Michele Calos

    Article

    Groth et al Genetics. 2004 Apr . 166(4):1775-82.

    Insert

    hygromycin gene driven by the copia promoter, phiC31 attB
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKB223

    Plasmid

    #170013

    Purpose

    Encodes LacI-controlled expression of surface-displayed C18G and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    C18G
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHG7

    Plasmid

    #188255

    Purpose

    Integrating plasmid contains the C31 int/attP locus and the hygromycin-resistance gene for selection

    Depositor

    Hong Gao, Maggie Smith

    Article

    Gao et al Access Microbiol. 2021 Dec 8;3(12):00029

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    act1p/actII-orf4

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCOLA-T7-YhjH

    Plasmid

    #79165

    Purpose

    Expresses YhjH under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    yhjH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEQ111m

    Plasmid

    #40190

    Depositor

    Paul Modrich

    Article

    Wright et al J Biol Chem. 1989 Jul 15;264(20):11816-2

    Insert

    EcoRI endonuclease & EcoRI methylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed Glutamic Acid 111 to Glutamine

    Promoter

    Lambda PL

    Available since

    Nov. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

26. pBAD33MevT

    Plasmid

    #17814

    Depositor

    Jay Keasling

    Article

    Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

    Insert

    MevT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHG5

    Plasmid

    #188253

    Purpose

    Integrating plasmid contains the SV1 int/attP locus and the kanamycin-resistance gene (aphII) for selection

    Depositor

    Hong Gao, Maggie Smith

    Article

    Gao et al Access Microbiol. 2021 Dec 8;3(12):00029

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    act1p/actII-orf4

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFF838

    Plasmid

    #61457

    Purpose

    aTc-inducible SCRIBE_lacZ(ON)

    Depositor

    Timothy Lu

    Article

    Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

    Insert

    SCRIBE_lacZ(ON)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pTetO

    Available since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PRM-GFP

    Plasmid

    #40127

    Depositor

    Michel Maharbiz

    Article

    Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

    Insert

    CI inducible sfGFP
    

    Use

    Synthetic Biology

    Tags

    LVA ssrA tag

    Expression

    Bacterial

    Mutation

    Promoter

    pRM

    Available since

    Sept. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJOE3075.3

    Plasmid

    #135084

    Purpose

    L-rhamnose-inducible expression vector

    Depositor

    Josef Altenbuchner

    Article

    Wegerer et al BMC Biotechnol. 2008 Jan 14;8:2. doi: 10

    Insert

    P-rhaP-BAD-malE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge