We narrowed to 9,280 results for: Pol

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pCellFree_G03 TFEC

Plasmid

#67060

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFEC (TFEC Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCHERRY10

Plasmid

#24664

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

mCherry

Expression

Bacterial

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
UMsfG

Plasmid

#161005

Purpose

ubiquitous expression of MCP-sfGFP

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

MCP-sfGFP

Expression

Plant

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU1-A-RFP

Plasmid

#72939

Purpose

Level 1 Destination vector - Position A

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 1 - A site

Use

Synthetic Biology

Mutation

Beta-lactamase - G717C (nucleotide) - silent muta…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5B-Flag-Par6 wt

Plasmid

#11748

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

PAR6 (Pard6a Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pQFlag-USP11 WT puroR

Plasmid

#46747

Purpose

Retroviral vector that expresses Flag-tagged wild type human USP11

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP11

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2B -938/+64

Plasmid

#24943

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only
pRK5myc RhoA L63

Plasmid

#15900

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

RhoA (RHOA Human)

Tags

myc

Expression

Mammalian

Mutation

Q63L

Available Since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
pMSP2

Plasmid

#26282

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP2

Tags

6-His

Expression

Bacterial

Mutation

synthetic gene of dimer of deletion mutant (1-43)…

Available Since

April 7, 2011

Availability

Academic Institutions and Nonprofits only
LifeAct-scramble

Plasmid

#217499

Purpose

Expresses LifeAct-mScarlet-scramble to serve as control plasmid for LifeAct-MTED

Depositor

Erik Dent

Article

Holland et al Mol Biol Cell. 2024 Jun 1;35(6):mr3. doi

Insert

LifeAct-mScarlet-scramble

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 TCF12

Plasmid

#67113

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TCF12 (TCF12 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
UPG

Plasmid

#161003

Purpose

ubiquitous expression of PCP-GFP

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PCP-GFP

Expression

Plant

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
IVT-MCP-MMLV-RT

Plasmid

#207457

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

MCP-RT

Expression

Bacterial

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPAGFP-TPX2

Plasmid

#31226

Depositor

Patricia Wadsworth

Article

Ma et al Mol Biol Cell. 2010 Mar 15. 21(6):979-88

Insert

TPX2 (TPX2 Human)

Tags

PAGFP

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pKI1

Plasmid

#199118

Purpose

Expresses N-terminally Hisx6-tagged, SNAP-tagged full-length RapA in E. coli cells, suitable for fluorescent labelling

Depositor

Jeff Gelles

Article

Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

Insert

H6-SNAP-RapA

Tags

6xHis and SNAP-tag

Expression

Bacterial

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-fullef1a-mutFAKSPARK2

Plasmid

#195266

Purpose

express Tyr to Phe mutant FAKSPARK2 in a lenti vector

Depositor

Xiaokun Shu

Article

Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

Insert

Tyr to Phe mutant FAKSPARK2 sensor

Use

Lentiviral

Expression

Mammalian

Promoter

ef1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-Muc2D1D2D3cysD1

Plasmid

#155214

Purpose

Expresses human MUC2 residues 21-1397

Depositor

Deborah Fass

Article

Javitt et al Cell. 2020 Oct 29;183(3):717-729.e16. do

Insert

Muc2 D1D2D3cysD1 (MUC2 Human)

Tags

6xHistadine

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRIME-CMV-GFP-recipient

Plasmid

#11657

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling GFP expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pDisplay-Gncp-iGluSnFR

Plasmid

#107337

Purpose

Noncircularly permutated (ncp) variant of iGluSnFR. Membrane-displayed.

Depositor

Robert Campbell

Article

Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

Insert

GltI based and noncircularly permutated glutamate biosensor

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pPMW-attB

Plasmid

#61814

Purpose

pUASp plasmid with attB for Drosophila transgenesis

Depositor

Frederique Peronnet

Article

Dardalhon-Cumenal et al PLoS Genet. 2018 Jul 11;14(7):e1007498.

Type

Empty backbone

Tags

6xMyc

Expression

Insect

Promoter

UASp

Available Since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
mFGF8a

Plasmid

#22088

Depositor

Gail Martin

Article

Crossley et al Development. 1995 Feb . 121(2):439-51.

Insert

FGF8 (Fgf8 Mouse)

Use

Bacterial

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
3TERM_35S

Plasmid

#136114

Purpose

3TERM after CDS/ CDS12-CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

3TERM_35S (CaMV)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSMF2

Plasmid

#112819

Purpose

Expresses wild-type Rpb1 in yeast cells

Depositor

Stephen Fuchs

Article

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Promoter

Native promoter

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
JME4390

Plasmid

#129656

Purpose

LEU2ex_CrisprCas9-yl_RFP: CAS9 vector with LEU2ex marker for gRNA cloning using GoldenGate

Depositor

Tristan Rossignol

Article

Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5myc Rac1 N17

Plasmid

#15904

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

Rac1 (RAC1 Human)

Tags

myc

Expression

Mammalian

Mutation

T17N

Available Since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
SiT-EGFP

Plasmid

#166943

Purpose

mammalian expression of N' Sialyl Transferase tagged with EGFP

Depositor

Jennifer Lippincott-Schwartz

Article

Patterson et al Cell. 2008 Jun 13;133(6):1055-67. doi: 1

Insert

Sialyl Transferase N-terminus

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
BHMT

Plasmid

#64075

Purpose

Expression of betaine homocysteine methyltransferase

Depositor

Vern Schramm

Article

Poulin et al J Org Chem. 2015 May 15;80(10):5344-7. d

Insert

betaine-homocysteine methyltransferase (BHMT Synthetic, Human)

Tags

none

Expression

Bacterial

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5myc Cdc42 wt

Plasmid

#15905

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

Cdc42 (placental isoform) (CDC42 Human)

Tags

myc

Expression

Mammalian

Available Since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
pBa.VAMP2-GFP-SBP

Plasmid

#180249

Purpose

Mammalian expression of VAMP2

Depositor

Marvin Bentley

Article

Nabb et al Mol Biol Cell. 2021 Nov 3:mbcE21080425.

Insert

VAMP2 (Vamp2 Rat)

Expression

Mammalian

Mutation

WT

Promoter

Chicken Beta actin

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
UBC13-PGEX4T

Plasmid

#25464

Depositor

Cynthia Wolberger

Article

Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

Insert

Ubc13 (UBC13, MMS2 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
pBP-SJM901

Plasmid

#72966

Purpose

Level 0 - SJM901 promoter (5' CTAT/3' GTAC fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

SJM901 promoter

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMD18-T-pyrF

Plasmid

#174388

Purpose

modified pMD18-T containing pyrF and its native promoter, Ampr

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

pyrF

Use

Template for cloning fragments for chromosomal in…

Expression

Bacterial

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 WWP1

Plasmid

#67125

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

WWP1 (WWP1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p.UTA.2.0 Empty

Plasmid

#82446

Purpose

[Edit] Dual Fluorescent endogenous miRNA sensor. To insert the complementary region at the 3'UTR of CFP

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Type

Empty backbone

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
CDS_mALS

Plasmid

#136081

Purpose

Confers plant resistance to chlorsulfuron

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS_mALS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pmNFM

Plasmid

#83126

Purpose

Mouse neurofilament protein M cDNA mammalian expression construct

Depositor

Anthony Brown

Article

Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):

Insert

mouse NFM (Nefm Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-EGFP-LacZshRNA

Plasmid

#73977

Purpose

LacZshRNA (mir155 backbone) was inserted into the 3'UTR of EGFP.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the LacZ gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 CYP2A13

Plasmid

#67103

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CYP2A13 (CYP2A13 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pAct::Raf

Plasmid

#11341

Depositor

Michael Wigler

Article

Van Aelst et al Proc Natl Acad Sci U S A. 1993 Jul 1. 90

Insert

byr-2 (byr2 Fission Yeast)

Tags

Gal4 activation domain

Expression

Yeast

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pQCXIP-NLS-Vx3-mEGFP

Plasmid

#35529

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

NLS-Vx3

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB143

Plasmid

#101679

Purpose

LAA-tagged mCherry under T7 promoter control

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

LAA-tagged mCherry under T7 promoter control

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5 MYC-Smurf2 (FF29/30A) WT

Plasmid

#24604

Depositor

Jeff Wrana

Article

Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

Insert

Smurf2 (SMURF2 Human)

Tags

MYC

Expression

Mammalian

Mutation

F29A, F30A

Available Since

April 30, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pZS220

Plasmid

#177642

Purpose

In vitro transcription of Patiria miniata Dishevelled

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled

Use

In vitro transcription

Tags

GFP

Mutation

K47A

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-2TK-PTB

Plasmid

#21929

Depositor

Phil Sharp

Article

Garcia-Blanco et al Genes Dev. 1989 Dec . 3(12A):1874-86.

Insert

PTB (PTBP1 Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-nEF-ConVERGD-eGFP-WPRE

Plasmid

#218747

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Promoter

nEF

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-nEF-ConVERGD-eGFP-W3SL

Plasmid

#218746

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Promoter

nEF

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
3TERM_bba_b0015_rev_chlo

Plasmid

#163931

Purpose

L0 part - terminator

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

from GenBank: KT364744.3, prokaryotic double terminator BBa_B0015

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCellFree_G03 TFAP2B

Plasmid

#67092

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFAP2B (TFAP2B Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Chx10 3kb AP (CC#318)

Plasmid

#15205

Depositor

Connie Cepko

Article

Rowan et al Dev Biol. 2005 May 15. 281(2):240-55.

Insert

Chx10 3kb AP reporter (Vsx2 Mouse)

Expression

Bacterial

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pRK5myc Rac1 L61

Plasmid

#15903

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

Rac1 (RAC1 Human)

Tags

myc

Expression

Mammalian

Mutation

Q61L

Available Since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only

Showing: 3651 - 3700 of 9280 results

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We narrowed to 9,280 results for: Pol