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Plasmid#106965Purposesingle-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screenDepositorInsertHypaSpCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFSAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Guide-EFS-SpCas9-P2A-EGFP
Plasmid#99248Purposesingle-vector system with EGFP reporter for use in CROPseq knockout screensDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsEGFPExpressionMammalianMutationPromoterEFSAvailable sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR.v2.TetON_Cas13d_U6_DR
Plasmid#196727PurposeSortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.DepositorInsertRfxCas13d-T2A-miRFP670nano
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationPromoterTREAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-EFS-HypaSpCas9-P2A-EGFP
Plasmid#106964Purposesingle-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screenDepositorInsertHypaSpCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFSAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-dCas9-VP64-MS2-VP64-ZsG
Plasmid#192665Purpose3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1DepositorInsertdCas9-VP64, MS2-VP64, ZsGreen1
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1aAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
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Lenti-EF1alpha-dCas9-VP64-MS2-VP64-Blast
Plasmid#192666Purpose3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A Blast resistance markerDepositorInsertdCas9-VP64, MS2-VP64
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1aAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-TurboCas-NLS-Blast
Plasmid#232346PurposeLentiviral expression vector for dCas9 fused to miniTurboNLSDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA, miniTurbo, V5 and 3xSV40 NLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cas9-GFP
Plasmid#86145PurposeLentiviral vector expressing Cas9-GFPDepositorInsertCas9-GFP
UseCRISPR and LentiviralTagsFLAG and GFPExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
FUGW-d2GFP-ZEB1 3'UTR
Plasmid#79601PurposeFUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.DepositorInsertd2GFP-Zeb1 3'UTR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Puro (658-5)
Plasmid#17448Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, PuroDepositorHas ServiceCloning Grade DNAInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_SV40_Large_T_Antigen-P2A-Hygro_Barcode
Plasmid#170255PurposeBarcoded lentiviral vector to express SV40 Large T Antigen in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertSV40 Large T Antigen
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti-mito-mTagBFP2
Plasmid#216130PurposeLentiviral vector for expressing mTagBFP2 localizing to mitochondria.DepositorInsertEF1a-COX8AmitochondrialLocalizationSignal-mTagBFP2-WPRE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.GFP
Plasmid#57822Purpose3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter drivenDepositorInsertssgRNA
EFS
eGFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV V5-LUC Blast (w567-1)
Plasmid#21474Purpose3rd gen lentiviral V5-Luciferase expression vector, CMV promoter, BlastDepositorInsertV5-LUC
UseLentiviral and LuciferaseTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLOX-TERT-iresTK
Plasmid#12245Purpose2nd generation lentiviral vector for reversible immortalization of human primary cellsDepositorInsertTERT, HSV1-TK (TERT Human)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationLoxP in 3' LTR.PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Neo (657-2)
Plasmid#17447Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, NeoDepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_humanIL-10_T2A_PDL1_PGK_mCherry
Plasmid#85430Purpose[Edit] Lentiviral vector for Gal4 inducible human IL-10/PD-L1 with constitutive mCherry expressionDepositorInserthuman IL-10 T2A PD-L1
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-LGALS3
Plasmid#175756PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS3DepositorInsertLGALS3 (LGALS3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-LGALS8
Plasmid#175758PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS8DepositorInsertLGALS8 (LGALS8 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only