8,809 results

1. TLCV2-RB1

   Plasmid

   #87836

   Purpose

   LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Insert

   sgRB1 (RB1 Human)
   

   Use

   CRISPR and Lentiviral; Doxycycline inducible; egf…

   Expression

   Mammalian

   Promoter

   Tight TRE promoter and U6

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-ihSyn1-tTA

   Plasmid

   #99120

   Purpose

   An AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTA

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Has Service

   AAV1

   Insert

   tTA
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   *(see below)

   Promoter

   ihSyn1

   Available Since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLAMP1-emiRFP670

   Plasmid

   #136570

   Purpose

   LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   LAMP1-emiRFP670
   

   Tags

   emiRFP670

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti CMV Blast DEST (706-1)

   Plasmid

   #17451

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Blast 

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.CMV.PI.EGFP.WPRE.bGH

   Plasmid

   #105530

   Purpose

   AAV expression of EGFP from CMV promoter

   Depositor

   James M. Wilson

   Has Service

   AAV PHP.eB, AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. E1b-GFP-Tol2

   Plasmid

   #37845

   Depositor

   Nadav Ahituv

   Article

   Birnbaum et al Genome Res. 2012 Jun;22(6):1059-68. Epub

   Type

   Empty backbone

   Use

   Zebrafish enhancer assay vector

   Promoter

   E1b

   Available Since

   Oct. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

   Plasmid

   #60904

   Purpose

   The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   scFv-GCN4
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF-GFP

   Plasmid

   #11154

   Purpose

   Mammalian expression vector for expression of GFP (EF1a promoter)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Has Service

   Cloning Grade DNA

   Insert

   EF1 alpha promoter (EEF1A1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. VAMP2-pHuji

   Plasmid

   #105289

   Purpose

   VAMP2 tagged with pH-sensitive red fluorescent protein pHuji

   Depositor

   Justin Taraska

   Article

   Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

   Insert

   VAMP2 (Vamp2 Rat)
   

   Tags

   pHuji

   Expression

   Mammalian

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSFFV_sfCherry2_TagBFP

    Plasmid

    #83031

    Purpose

    Expresses full length sfCherry2 and TagBFP fusion in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry+full length_TagBFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    3 mutations in sfCherry full length: E118Q, T128I…

    Promoter

    SFFV

    Available Since

    Jan. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. TetO-FUW-NfiB

    Plasmid

    #64900

    Purpose

    Inducible expression of murine CDS encoding for NfiB. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. cells.

    Depositor

    Vania Broccoli

    Article

    Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

    Insert

    NfiB (Nfib Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBMN-YFP-Parkin

    Plasmid

    #59416

    Purpose

    to make YFP-Parkin stable cell line with retrovirus

    Depositor

    Richard Youle

    Article

    Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

    Insert

    YFP-Parkin (PRKN Human)
    

    Use

    Retroviral

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-AKAR4

    Plasmid

    #61619

    Purpose

    FRET-based biosensor for monitoring PKA activity

    Depositor

    Jin Zhang

    Article

    Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

    Insert

    AKAR4
    

    Tags

    Cerulean and cpVenus[E172]

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-GFP-MDC1 (770-7)

    Plasmid

    #26284

    Depositor

    Eric Campeau

    Insert

    Mediator of DNA damage checkpoint 1 (MDC1 Human)
    

    Use

    Entry vector

    Tags

    GFP

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHes1(2.5k)-luc

    Plasmid

    #43806

    Depositor

    Ryoichiro Kageyama

    Article

    Takebayashi et al J Biol Chem. 1994 Feb 18;269(7):5150-6.

    Insert

    Hes1 Promoter (Hes1 Mouse)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains the murine Hes1 promoter

    Promoter

    Hes1 Promoter

    Available Since

    March 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLv-CMV-MyoD-ER(T)

    Plasmid

    #26809

    Depositor

    Jeffrey Chamberlain

    Article

    Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

    Insert

    MyoD-ER(T) (Myod1 Mouse)
    

    Use

    Lentiviral

    Available Since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pC0046-EF1a-PspCas13b-NES-HIV

    Plasmid

    #103862

    Purpose

    Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    PspCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and HIV NES

    Expression

    Mammalian

    Available Since

    Nov. 28, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pLJC5-LAMP1-RFP-2xFlag

    Plasmid

    #102931

    Purpose

    Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-Flag)

    Depositor

    David Sabatini

    Article

    Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

    Insert

    LAMP1
    

    Use

    Lentiviral

    Tags

    mRFP-2xFlag

    Promoter

    ubc

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223-PDGFRA

    Plasmid

    #23892

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PDGFRA (PDGFRA Human)
    

    Use

    Gateway donor vector

    Mutation

    M260I

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. ppyCAG_RNaseH1_D210N

    Plasmid

    #111904

    Purpose

    Express D210N mutated RNASEH1 which abolishes the catalytic activity of RNASEH1

    Depositor

    Xiang-Dong Fu

    Article

    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

    Insert

    RNASEH1 (RNASEH1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Changed D 210 to N

    Available Since

    July 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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