8,723 results

1. pET-MBP-mSA2

   Plasmid

   #52319

   Purpose

   Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

   Depositor

   Sheldon Park

   Article

   Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

   Insert

   monomeric streptavidin 2
   

   Use

   Tags

   FLAG, His, MBP, and TEV

   Expression

   Bacterial

   Mutation

   S25H mutation compared to streptavidin

   Promoter

   T7

   Available since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-Luc

   Plasmid

   #45968

   Depositor

   Ralf Kuehn

   Insert

   Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-puro-cOVA

   Plasmid

   #135073

   Purpose

   Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance

   Depositor

   Maria Castro

   Insert

   cOVA (OVAL Chicken)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-dCas9-VP64-6xHis

   Plasmid

   #62935

   Purpose

   Expression of dCas9-VP64-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   dCas9-VP64
   

   Use

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A and H840A amino acid changes render Cas9 nuc…

   Promoter

   T7

   Available since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBtet-Pur

   Plasmid

   #60507

   Purpose

   SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and puromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TCE & RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. IL13 promoter luciferase

   Plasmid

   #11784

   Depositor

   Anjana Rao

   Article

   Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

   Insert

   IL-13 promoter (IL13 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 4, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pCAX APP Swe/Ind

   Plasmid

   #30145

   Depositor

   Dennis Selkoe, Tracy Young-Pearse

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   human APP 695 Swedish/Indiana mutation (APP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Swedish and Indiana; K595N, M596L and V642F

   Promoter

   Available since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-C1 mCherry-NLS

   Plasmid

   #58476

   Purpose

   Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

   Depositor

   Dyche Mullins

   Article

   Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

   Insert

   3X NLS with 3X stop codons
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti PGK Hygro DEST (w530-1)

   Plasmid

   #19066

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Hygro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZS2-123

    Plasmid

    #26598

    Depositor

    Michael Elowitz

    Article

    Cox RS et al J Biol Eng. 2010 Jul 21;4:10. doi: 10.11

    Insert

    Synthetic
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW57-MCS1-P2A-MCS2 (GFP)

    Plasmid

    #80924

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. FKBP-V5-AP-nes_pLX304

    Plasmid

    #120912

    Purpose

    Lentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosol

    Depositor

    Alice Ting

    Article

    Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

    Insert

    FKBP-V5-AP-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. CROP-seq-opti

    Plasmid

    #106280

    Purpose

    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.

    Depositor

    Jay Shendure

    Article

    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

    Insert

    EF1a-Puro-WPRE-hU6-gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. C1(1-29)-TurboID-V5_pCDNA3

    Plasmid

    #107173

    Purpose

    expresses V5-tagged TurboID on the mammalian ER membrane

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3-RARE-luciferase

    Plasmid

    #13458

    Depositor

    T. Michael Underhill

    Article

    Hoffman et al J Cell Biol. 2006 Jul 3. 174(1):101-13.

    Insert

    Retinoic Acid Receptor Response Element
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX458M-53BP1-DN1S

    Plasmid

    #131045

    Purpose

    Plasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.

    Depositor

    Punam Malik

    Article

    Jayavaradhan et al Nat Commun. 2019 Jun 28;10(1):2866. doi:

    Insert

    SpCas9-53BP1-DN1S (TP53BP1 Human)
    

    Use

    CRISPR

    Tags

    GFP and SpCas9

    Expression

    Mammalian

    Mutation

    Only expressing residues 1231-1644 of complete 53…

    Promoter

    CBH

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAV0286

    Plasmid

    #149333

    Purpose

    Bacterial expression of SENP_EuB protease

    Depositor

    Dirk Görlich

    Article

    Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

    Insert

    SENP_EuB protease
    

    Use

    Tags

    His14 and Tev protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-hLC3B-pcDNA3.1

    Plasmid

    #40827

    Depositor

    David Rubinsztein

    Article

    Jahreiss et al Traffic. 2008 Apr;9(4):574-87. Epub 2008

    Insert

    LC3B (MAP1LC3B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. v3em-Cterm-PE2max-∆RNaseH-dualU6

    Plasmid

    #198735

    Purpose

    AAV genome encoding C-terminal PE2max and U6 expression cassettes

    Depositor

    David Liu

    Article

    Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

    Insert

    NpuC-CtermPE2max∆RNaseH
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Cbh

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. NYESO beta/alpha into TRAC HDRT Source (pTR 169)

    Plasmid

    #112021

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO beta/alpha into TRAC HDRT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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