We narrowed to 3,063 results for: ER
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Plasmid#99049PurposeTranscription Factor chassis for activation domains. mCherry, Zif268 DNA binding domain, estrogen response domainDepositorInsertsExpressionYeastAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only
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pOUPc-UL148-mCherry
Plasmid#179279PurposeAll in one "tet-on" lentivirus vector that expresses human cytomegalovirus UL148 fused to an mCherry tag at its cytoplasmic tail. UL148 reorganizes the ER and activates the UPR.DepositorInsertUL148 fused to mCherry
UseLentiviral; Tet-on, all-in-one lentiviral vectorTagsmCherryExpressionMammalianPromoterTet responsive element 3GAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBT3000
Plasmid#122536PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (tram-1) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_B2AR-i-pep
Plasmid#47436PurposeFRET sensor for beta2-adrenergic receptorDepositorInsertbeta2-adrenergic receptor (ADRB2 Human)
Tags10 nm ER/K helix, HA, i-pep, mCerulean, and mCitr…ExpressionMammalianPromoterpCMVAvailable SinceSept. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBT3003
Plasmid#122537PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (pisy-1) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
dCas9-NLS-gs10-cERb447
Plasmid#188257PurposePlasmid ensures constitutive expression of the C-terminal fragment of split ERbeta (aa 447-501), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminusDepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
Str-STIM1-NN_puromycin
Plasmid#65310PurposeLuminal ER hook only (RUSH system)DepositorInsertSTIM1 mutated for binding motif to microtubule (STIM1 Human)
TagsstreptavidinExpressionMammalianMutation2 amino acids of STIM1 (IP from SxIP motif) were …PromoterCMVAvailable SinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Sec22b-shR
Plasmid#208358PurposeMammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22bDepositorInsertSec22b (Sec22b Rat)
TagsEGFPExpressionMammalianMutationfour silent mutations introduced to render shRNA …PromoterCMVAvailable SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_B2AR-no-pep
Plasmid#47439Purposecontrol FRET sensor for beta2-adrenergic receptorDepositorInsertbeta2-adrenergic receptor (ADRB2 Human)
Tags10 nm ER/K helix, HA, mCerulean, mCitrine, and no…ExpressionMammalianPromoterpCMVAvailable SinceSept. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_B2AR-q-pep
Plasmid#47437PurposeFRET sensor for beta2-adrenergic receptorDepositorInsertbeta2-adrenergic receptor (ADRB2 Human)
Tags10 nm ER/K helix, HA, mCerulean, mCitrine, and q-…ExpressionMammalianPromoterpCMVAvailable SinceSept. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-mGreenLantern
Plasmid#164467PurposeVisualization of the ER in cells (compatible with OSER assay)DepositorInsertmGreenLantern
TagsCytochrome p450 first 29 animo acids (Oryctolagus…ExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ESR1_Hormone-recep
Plasmid#109906PurposeProtein expression and purification of ESR1_Hormone-recepDepositorInsertESR1_Hormone-recep (ESR1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-FKBP-CYB5Atail
Plasmid#108141PurposeFor recruitment of ERDepositorExpressionMammalianMutationCYB5A(100-134)PromoterCMVAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nanog-CreER targeting construct
Plasmid#59720PurposeCre-ER knockin targeting construct for mouse endogenous Nanog locusDepositorInsertcreERT2
UseMouse TargetingPromotermNanogAvailable SinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOUPc-UL148-HA
Plasmid#179343PurposeAll-in-one "tet-on" 3G lentiviral vector plasmid encoding human cytomegalovirus UL148 ER resident glycoprotein with a C-terminal HA tag fused to its cytoplasmic tailDepositorInserthuman cytomegalovirus UL148 fused to HA epitope
UseLentiviral and Synthetic Biology; Hiv-1 based rep…TagsHA tagExpressionMammalianMutationinsert matches UL148 from strain TB40/EPromoterTet responsive element 3GAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ESR2_Hormone-recep
Plasmid#109907PurposeProtein expression and purification of ESR2_Hormone-recepDepositorInsertESR2_Hormone-recep (ESR2 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3
Plasmid#221112PurposeFAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ERDepositorInsertMFA-IgKappa-FAPoptim-STE3
ExpressionYeastPromoterSTE3Available SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-CCR5
Plasmid#222309PurposeSynchronize the trafficking of CCR5 from the ER.DepositorAvailable SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-CCR5-Cys3A
Plasmid#222313PurposeSynchronize the trafficking of CCR5-Cys3A from the ER.DepositorInsertStreptavidin-KDEL and CCR5-Cys3A fused to SBP-EGFP (CCR5 Human)
ExpressionMammalianMutationC321A, C323A, C324APromoterCMVAvailable SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-LAMP1-Y414A
Plasmid#222319PurposeSynchronize the trafficking of LAMP1-Y414A from the ER.DepositorInsertStreptavidin-KDEL and LAMP1-Y414A fused to SBP-EGFP (LAMP1 Human)
ExpressionMammalianMutationTyr414 to AlaPromoterCMVAvailable SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-mCherry-LAMP1-Y414A
Plasmid#222320PurposeSynchronize the trafficking of LAMP1-A17 from the ER.DepositorInsertStreptavidin-KDEL and LAMP1-Y414A fused to SBP-mCherry (LAMP1 Human)
ExpressionMammalianPromoterCMVAvailable SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-LAP
Plasmid#222322PurposeSynchronize the trafficking of LAP from the ER.DepositorAvailable SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-EGFR
Plasmid#222325PurposeSynchronize the trafficking of EGFR from the ER.DepositorAvailable SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA245 CMVTO_ERret-H2M-Renin_P2A_mRens-hIL6-rens.1_T2A_mRens-cageIL-10-c-myc_T2A-mCherry
Plasmid#231206PurposeSingle transcript cageIL-10 (mRens) + cleaveIL-6 (mRens) + ER-renin (H2M)DepositorInsertERret-H2M-Renin_P2A_mRens-hIL6-rens.1_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)
ExpressionMammalianMutationL147I, R148H, I203V, L290VAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0248 EF1a_mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry_Gen2
Plasmid#231208PurposeSingle transcript cageIL-2 (mRens) + ER-renin (H2M) (Lenti)DepositorInsertmRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry (IL2 Human)
UseLentiviralMutationL80F, R81D, L85V, I86V, I92FAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA244 CMVTO_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry
Plasmid#231205PurposeSingle transcript cageIL-10 (mRens) + ER-renin (H2M)DepositorInsertERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)
ExpressionMammalianMutationL147I, R148H, I203V, L290VAvailable SinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0266 CMVTO-IVT-UTR-mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin (FLP-IN)
Plasmid#231209PurposeSingle transcript mRNA cageIL-2 (mRens) + ER-renin (H2M)DepositorInsertUTR-mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin (IL2 Human)
ExpressionMammalianMutationL80F, R81D, L85V, I86V, I92FAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0230 EF1a_Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry_Gen2
Plasmid#231207PurposeSingle transcript cageIL-10 (mRens) + ER-renin (H2M) (Lenti)DepositorInsertPuro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)
UseLentiviralMutationL147I, R148H, I203V, L290VAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-EREG-mNeonGreen
Plasmid#209914PurposeTo monitor the expression of Epiregulin, the plasmid encodes a recombinant Epiregulin fused intracellularly to the mNeonGreen fluorescent proteinDepositorAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
mApple-1xHp
Plasmid#214409PurposeLipid droplet and ER localization of 1xHp of Spastin fused to mAppleDepositorAvailable SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1_B1ChR2_TS_mScarlet_ER
Plasmid#195191PurposeHighly K+ selective channelrhodopsinDepositorInsertBilabrum sp. K+ selective channelrhodopsin 2
TagsER-export sequence, Kir2.1 trafficking motif, and…ExpressionMammalianPromoterCMVAvailable SinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
vPyACR_2164382
Plasmid#153029PurposeAnion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).DepositorInsertvPyACR_2164382
TagseYFPExpressionMammalianMutationvPyACR_2164382 was C-terminally truncated to incl…PromoterCMV (+ enhancer)Available SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
vPyACR_21821
Plasmid#153030PurposeAnion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).DepositorInsertvPyACR_21821
TagseYFPExpressionMammalianMutationvPyACR_21821 was C-terminally truncated to includ…PromoterCMV (+ enhancer)Available SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-Cerulean3-Cerulean3-FLARE-D1ER
Plasmid#123354PurposeCyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator, targeted to endoplasmic reticulum lumen.DepositorInsertcp-moxCerulean3-moxCerulean3-FLARE-D1ER
TagsC-terminal ER retention signal (KDEL), cp-moxCeru…ExpressionMammalianPromoterCMVAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOUPc-Rh159-GFP
Plasmid#179280PurposeAll in one "tet-on" lentivirus vector that expresses rhesus cytomegalovirus Rh159 fused to an eGFP tag at its cytoplasmic tail. Rh159 is an ER resident protein that binds NKG2D activating ligands.DepositorInsertRh159 fused to eGFP
UseLentiviral; All-in-one "tet" on lentivi…TagseGFPExpressionMammalianPromoterTet Responsive Element 3GAvailabilityAcademic Institutions and Nonprofits only -
pCMV-GenEPi
Plasmid#140236PurposeMammalian expression of Piezo1-based fluorescent force sensor GenEPiDepositorInsertGenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)
TagsGCaMP 6s RS1 EF4ExpressionMammalianPromotersimian CMV IE94Available SinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
3HA-ERHBD-Hoxb8
Plasmid#212932PurposeExpresses an estrogen-regulated form of Hoxb8 to generate conditionally immortalized hematopoietic progenitor cell linesDepositorUseRetroviralTagsTriple HA tagExpressionMammalianPromoterMSCV LTRAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hERalpha
Plasmid#101141PurposeMammalian expression of full-length human estrogen receptor-alphaDepositorAvailable SinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-XBP1 mNeonGreen NLS
Plasmid#115971PurposeER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen (retroviral vector backbone)DepositorInsertX-box binding protein 1 (XBP1 Human, Synthetic)
UseRetroviralTagsHA tag, c-myc NLS, and mNeonGreenExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_CD63-SBP-mCherry
Plasmid#222326PurposeSynchronize the trafficking of CD63 from the ER.DepositorInsertStreptavidin-KDEL and CD63 fused to SBP-mCherry (CD63 Human)
ExpressionMammalianPromoterCMVAvailable SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only