We narrowed to 13,482 results for: cas9 genes

1. pEM-eCas9-D1135E

   Plasmid

   #154926

   Purpose

   aTc inducible eCas9 with D1135E mutation

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   eCas9-D1135E
   

   Use

   CRISPR and Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A, D1135E

   Promoter

   pTet

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEM-OptiCas9-D1135E

   Plasmid

   #154928

   Purpose

   aTc inducible OptiCas9 with D1135E mutation

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   OptiCas9-D1135E
   

   Use

   CRISPR and Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   R661A, K1003H, D1135E

   Promoter

   pTet

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEM-HypaCas9-D1135E

   Plasmid

   #154930

   Purpose

   aTc inducible HypaCas9 with D1135E mutation

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   HypaCas9-D1135E
   

   Use

   CRISPR and Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   N692A/M694A/Q695A/H698A/D1135E

   Promoter

   pTet

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEM-Cas9-D1135E

   Plasmid

   #154931

   Purpose

   aTc inducible Cas9 with D1135E mutation

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   Cas9-D1135E
   

   Use

   CRISPR and Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   D1135E

   Promoter

   pTet

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEM-Cas9HF1-D1135E

   Plasmid

   #154924

   Purpose

   aTc inducible Cas9HF1 with D1135E mutation

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   Cas9HF1-D1135E
   

   Use

   CRISPR and Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A/D1135E and stop codon on …

   Promoter

   pTet

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_eA3A T31A-NG-nCas9

   Plasmid

   #163539

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A T31A-NG-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…

   Promoter

   Available since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_eA3A T31A-nCas9

   Plasmid

   #163538

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A T31A-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)eA3A T31A

   Promoter

   Available since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_UdgX-EE-UdgX-nCas9

   Plasmid

   #163564

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-EE-UdgX-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)

   Promoter

   Available since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. miniABEMax V82G nSaCas9

   Plasmid

   #135365

   Purpose

   Expression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitution

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   miniABEMax V82G nSaCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. UAS-u(XXL)Cas9

    Plasmid

    #127387

    Purpose

    Expresses Cas9 at very low level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF(XXL)-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. eSpCas9(1.1)-Cdt1

    Plasmid

    #122342

    Purpose

    Expresses sgRNA targeting mouse Cdt1 and eSpCas9 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Cdt1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-FLAG-B-eSpCas9

    Plasmid

    #126772

    Purpose

    Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-eSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    K848A; K1003A; R1060A; amino acids 1005-1013 repl…

    Promoter

    T7

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. BII-ChPtW-eSpCas9

    Plasmid

    #133381

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    eSpCas9
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    eSpCas9 mutations

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJUMP29[dCas9]-1A(lacZ)

    Plasmid

    #126979

    Purpose

    Level 1 vector. With lacZ as alternative cloning reporter.OriV 9 (pBBR322/ROP; medium copy number). Constitutive dCas9 in downstream secondary site.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Cas9 catalytically dead

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Native promoter dCas9

    Plasmid

    #113656

    Purpose

    dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.

    Depositor

    Jeffrey Barrick

    Article

    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

    Insert

    Native promoter and dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    S. pyogenes Cas9 native promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX335A_hCas9(D10A)_IRF8gRNA4

    Plasmid

    #89722

    Purpose

    Knockout IRF8 in human cells

    Depositor

    Martin Zenke

    Article

    Sontag et al Stem Cells. 2017 Jan 16. doi: 10.1002/st

    Insert

    IRF8 gRNA4 (IRF8 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX335A_hCas9(D10A)_IRF8gRNA3

    Plasmid

    #89721

    Purpose

    Knockout IRF8 in human cells

    Depositor

    Martin Zenke

    Article

    Sontag et al Stem Cells. 2017 Jan 16. doi: 10.1002/st

    Insert

    IRF8 gRNA3 (IRF8 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQdCas9.luxR(mut)-sggfp

    Plasmid

    #236185

    Purpose

    The plasmid pQdCas9.luxR(mut)-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the GFP gene. Additionally, this plasmid contains a mutation in the luxR gene.

    Depositor

    Radhakrishnan Mahadevan

    Insert

    Quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Quorum sensing promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAVS1-tet-iCas9-BFP2

    Plasmid

    #125519

    Purpose

    all-in-one-inducible Cas9

    Depositor

    Jason Moffat

    Article

    Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

    Insert

    AAVS1-TET-ON 3G-SpCas9-P2A-BFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMOD_A-CmYLCV-dCas9-24XGP41

    Plasmid

    #202009

    Purpose

    Moclo level 1 - Module A, Promoter: CmYLCV, Gene: dCas9-24XGP41, Terminator: HSP

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Insert

    dCas9-24XGP41
    

    Use

    Synthetic Biology; Moclo level 1 vector

    Tags

    GS linkers

    Expression

    Mutation

    Promoter

    CmYLCV

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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