163,584 results

1. pBetaActin-TUBB2a-mScarlet

   Plasmid

   #191324

   Purpose

   Expresses fluorescent labeled beta Tubulin to measure microtubule density

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   TUBB2a-mScarlet (Tubb2a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCEP4-myc-PDGFRA-K627R_EGFP-PEST_reporter

   Plasmid

   #172597

   Purpose

   Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R mutant signaling.

   Depositor

   Daniel Kiss

   Article

   Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

   Insert

   myc-PDGFRA-K627R (PDGFRA Human, Synthetic)
   

   Use

   Egfp-pest_reporter

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   PDGFRA-K627R

   Promoter

   CMV

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-sgTp53#3/Cre

   Plasmid

   #173622

   Purpose

   Expresses a Tp53-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Tp53 (Trp53 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-sgBrca2#2/Cre

   Plasmid

   #173628

   Purpose

   Expresses a Brca2-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Brca2 (Brca2 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-sgPten#1/Cre

   Plasmid

   #173645

   Purpose

   Expresses a Pten-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Pten (Pten Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLyGo-Bs-1

   Plasmid

   #163137

   Purpose

   pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PamyL-PamyQ-PcryIIIA-cryIIIAstab49

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACYCDuet1-tglB

   Plasmid

   #134676

   Purpose

   Expresses His6-TglBin E. Coli

   Depositor

   Wilfred van der Donk

   Article

   Ting et al Science. 2019 Jul 19;365(6450):280-284.

   Insert

   TglB
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-UbC-mNeonGreen

   Plasmid

   #124102

   Purpose

   Expression of the fluorescent protein mNeonGreen in mammalian cells

   Depositor

   Pantelis Tsoulfas

   Insert

   mNeonGreen
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Codon usage was optimized for Homo Sapiens

   Promoter

   UbC

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. [#GS15-LV] DsRed GFP TEVp Blast Switch - Recipient

   Plasmid

   #233506

   Purpose

   Lentiviral construct for the MitoTRACER genetic reporter to be expressed in the recipient cells

   Depositor

   Simon Grelet

   Article

   Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

   Insert

   DsRed loxp eGFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. mEGFP-N1

    Plasmid

    #54767

    Purpose

    Localization: N1 Cloning Vector, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pgRNA-bacteria

    Plasmid

    #44251

    Purpose

    Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    guide RNA (bacteria)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available Since

    April 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV_ENVLPE+

    Plasmid

    #232427

    Purpose

    Expression plasmid to produce ENVLPE+ particles

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    ENVLPE+
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    2xCMV

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-CMV-Blast-PIP-FUCCI

    Plasmid

    #138715

    Purpose

    Lentiviral expression of the cell cycle marker PIP-FUCCI

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    PIP-FUCCI
    

    Use

    Lentiviral

    Tags

    mCherry and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. TLCV2

    Plasmid

    #87360

    Purpose

    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Insert

    Cas9-2A-eGFP
    

    Use

    CRISPR and Lentiviral; Doxycycline inducible; egf…

    Tags

    Cas9-T2A-eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBOB-EF1-FastFUCCI-Puro

    Plasmid

    #86849

    Purpose

    3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection

    Depositor

    Duncan Jodrell, Kevin Brindle

    Article

    Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164

    Inserts

    mKO2-hCDT1(30-120)
    mAG-hGEM(1-110)
    pac (puromycin N-acetyl-transferase)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 and PGK

    Available Since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXPR_502

    Plasmid

    #96923

    Purpose

    for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF

    Depositor

    John Doench, David Root

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-Flag

    Plasmid

    #208051

    Purpose

    Empty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with Flag

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. ABE8e-Cas9NG

    Plasmid

    #203328

    Purpose

    Plasmid for bacterial purification of codon optimized ABE8e-Cas9NG

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

    Insert

    ABE8e-Cas9NG
    

    Use

    CRISPR

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMBP-LbCas12a

    Plasmid

    #113431

    Purpose

    Expresses 10xHis-MBP fused to LbCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Has Service

    Cloning Grade DNA

    Insert

    LbCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. L4440

    Plasmid

    #1654

    Depositor

    Andrew Fire

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    RNAi

    Tags

    T7p, T7p, lacZN, OriF1>>, OriF1<<

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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