We narrowed to 16,751 results for: EMB

1. Hygro-P2A-HA-FKBP-WAPL

   Plasmid

   #217666

   Purpose

   Repair template to endogenously tag WAPL with Hygro-P2A-HA-FKBP

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   WapI (WAPL Human)
   

   Use

   Tags

   dTag (FKBP36V protein)

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Lac

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAc5.1B-lambdaN-HA-DmAGO1-L819Y-siRNAres_AC

   Plasmid

   #148394

   Purpose

   Insect Expression of DmAGO1-L819Y-siRNAres

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   DmAGO1-L819Y-siRNAres (AGO1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   variation with 2 non silent mutations compared to…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAc5.1B-lambdaN-HA-DmAGO1-P714GA744FL819Y-siRNAres_AC

   Plasmid

   #148395

   Purpose

   Insect Expression of DmAGO1-P714GA744FL819Y-siRNAres

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   DmAGO1-P714GA744FL819Y-siRNAres (AGO1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   variation with 2 silent mutations compared to the…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAc5.1B-lambdaN-HA-DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNAres_AC

   Plasmid

   #148404

   Purpose

   Insect Expression of DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNares

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNares (Not1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   10 silent mutations and two non silent N605S, K75…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-MS2-HA-HsNot1iso1_1318-1605-shRNAres_AC

   Plasmid

   #148414

   Purpose

   Mammalian Expression of HsNot1iso1_1318-1605-shRNAres

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   HsNot1iso1_1318-1605-shRNAres (CNOT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1 silent mutation compared to the sequence of iso…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCIneo-lambdaN-HA-HsTNRC6A_1-1609-del1384-1524-siRNAres_N

   Plasmid

   #147024

   Purpose

   Mammalian Expression of HsTNRC6A_1-1609-del1384-1542-siRNAres

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

   Insert

   HsTNRC6A_1-1609-del1384-1542-siRNAres (TNRC6A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one non silent mutation K1578E compared to the se…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAc5.1B-lambdaN-DmPABPC1-siRNAres-HA_O

   Plasmid

   #147097

   Purpose

   Insect Expression of DmPABPC1-siRNAres

   Depositor

   Elisa Izaurralde

   Article

   Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

   Insert

   DmPABPC1-siRNAres (pAbp Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   seven silent mutations compared to the sequence g…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1B-lambdaN-DmPABPC1_1-550-siRNAres_L

   Plasmid

   #146859

   Purpose

   Insect Expression of DmPABPC1_1-550-siRNAres

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

   Insert

   DmPABPC1_1-550-siRNAres (pAbp Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one non silent mutation G26S, and six silent muta…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAc5.1B-lambdaN-DmPABPC1_90-635-siRNAres-V5His6_L

   Plasmid

   #146860

   Purpose

   Insect Expression of DmPABPC1_90-635-siRNAres

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

   Insert

   DmPABPC1_90-635-siRNAres (pAbp Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   five silent mutations compared to the sequence gi…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAc5.1B-lambdaN-DmPABPC1-del91-164-V5His6_L

    Plasmid

    #146861

    Purpose

    Insect Expression of DmPABPC1_del91-164

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

    Insert

    DmPABPC1_del91-164 (pAbp Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one non silent mutation G26S, and five silent mut…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAc5.1B-EGFP-DmGW182_1-1199-del862-1115-dsRNAres_L

    Plasmid

    #146820

    Purpose

    Insect Expression of DmGW182_1-1199-del862-1115-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

    Insert

    DmGW182_1-1199-del862-1115-dsRNAres (gw Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Two non silent mutations V704A and N799S and one …

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCIneo-lambdaN-HA-HsTNRC6A-del1352-1369-siRNAres_K

    Plasmid

    #146743

    Purpose

    Mammalian Expression of HsTNRC6A-del1352-1369-siRNAres

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

    Insert

    HsTNRC6A-del1352-1369-siRNAres (TNRC6A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one non silent mutation K1578E compared to the se…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RPS4X sgRNA-3 lentiCRISPR v2-Blast plasmid

    Plasmid

    #192233

    Purpose

    lentiviral vector expressing sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RPS4X sgRNA-4 lentiCRISPR v2-Blast plasmid

    Plasmid

    #192234

    Purpose

    lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RPS4X sgRNA-2 lentiCRISPR v2 plasmid

    Plasmid

    #192232

    Purpose

    lentiviral vector expressing sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RPS4X sgRNA-1 lentiCRISPR v2 plasmid

    Plasmid

    #192231

    Purpose

    lentiviral vector expressing sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV_pMBP-dnVamp3-P2AT2A-EGFP-caax

    Plasmid

    #190154

    Purpose

    Expresses dominant-negative Vamp3 (rat Vamp3 AA 1-81) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

    Depositor

    Brad Zuchero

    Article

    Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

    Insert

    Vamp3 (Vamp2 Rat)
    

    Use

    AAV

    Tags

    P2AT2A-EGFP-caax

    Expression

    Mammalian

    Mutation

    Truncation that includes only rat Vamp3 AA #1-81

    Promoter

    MBP

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLNHA-C1-HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres_AC

    Plasmid

    #148442

    Purpose

    Mammalian Expression of HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres (CNOT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1 silent mutation compared to the sequence of iso…

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGGS-chPKCdelta/eDHFR(69K6)-EGFP

    Plasmid

    #178857

    Purpose

    Mammalian expression of PKCδ-eDHFR(69K6) chimera fused to EGFP

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    PKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-EGFP (PRKCD Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS-chPKCdelta/eDHFR(69K6)-miRFP670

    Plasmid

    #178858

    Purpose

    Mammalian expression of PKCδ-eDHFR(69K6) chimera fused to miRFP670

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    PKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-miRFP670 (PRKCD Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJET-62-SFFV-T2A-PuroR-polyA-R11b

    Plasmid

    #179893

    Purpose

    Donor with homologous arms for Rab11b to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11B, member RAS oncogene family (RAB11B Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJET-64-SFFV-T2A-BlaR-polyA-R11b

    Plasmid

    #179895

    Purpose

    Donor with homologous arms for Rab11b to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11B, member RAS oncogene family (RAB11B Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCIneo-lambdaN-HA-HsTNRC6A_1242-1709-F1359A_K

    Plasmid

    #146742

    Purpose

    Mammalian Expression of HsTNRC6A_1242-1709-F1359A

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

    Insert

    HsTNRC6A_1242-1709-F1359A (TNRC6A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    five non silent mutations D1289G, G1515A, S1516G,…

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pIRESneo2 mycBioID-Tollip M240A,F241A

    Plasmid

    #178055

    Purpose

    myc-BioID fused to human Tollip carrying CUE domain mutations cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.

    Depositor

    David Tumbarello

    Article

    Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

    Insert

    Toll-Interacting Protein (TOLLIP Human)
    

    Use

    Tags

    mycBioID

    Expression

    Mammalian

    Mutation

    Changed Methionine 240 to Alanine and changed Phe…

    Promoter

    CMV

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FUS_LC_charge_RtoK

    Plasmid

    #139127

    Purpose

    express FUS LC with hnRNPA2-like charge patterning where all R are changed to K

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Add K and D to FUS LC so it has the same charge p…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TPC2(E643A)-mCherry

    Plasmid

    #135187

    Purpose

    Mutant TPC2 with reduced Ca2+ selectivity, tagged on C terminus with mCherry.

    Depositor

    Antony Galione

    Article

    Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

    Insert

    TPC2 (Tpcn2 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Mutation of glutamate 643 to alanine

    Promoter

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FB026

    Plasmid

    #119711

    Purpose

    Transcriptional Unit (TU) for YFP expression, obtained by combining FB/GBparts FB007+GB0053+FB008 into pDGB3alpha1R. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    Promoter gpdA:YFP coding sequence:Terminator trpC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKK-TEV-mCardinal

    Plasmid

    #105798

    Purpose

    Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKK-TEV-mCerulean

    Plasmid

    #105790

    Purpose

    Expression of your protein of interest in fusion with cyan fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKK-mCardinal-TEV

    Plasmid

    #105781

    Purpose

    Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    mCardinal-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pKK-mCerulean-TEV

    Plasmid

    #105773

    Purpose

    Expression of your protein of interest in fusion with cyan fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    mCerulean-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. KLHL31 pgk (1088)

    Plasmid

    #62031

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    KLHL31 (Klhl31 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

33. pcDNA3/hArf6(Q67L)-HA

    Plasmid

    #79425

    Purpose

    Expresses C-terminally HA-tagged Arf6(Q67L) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Glutamine 67 to Leucine

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3/hArf6(T27N)-HA

    Plasmid

    #79426

    Purpose

    Expresses C-terminally HA-tagged Arf6(T27N) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Threonine 27 to Asparagine

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKK-BiFC-Venus

    Plasmid

    #105804

    Purpose

    Concomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    VN173 (I152L); VC155

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3/hArf1(WT)-mCherry

    Plasmid

    #79419

    Purpose

    Expresses C-terminally mCherry-tagged Arf1(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf1 (ARF1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3/hArf3(WT)-mCherry

    Plasmid

    #79420

    Purpose

    Expresses C-terminally mCherry-tagged Arf3(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf3 (ARF3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pKK-TEV-EGFP

    Plasmid

    #105793

    Purpose

    Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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39. TRPML1-mCherry

    Plasmid

    #135189

    Purpose

    Expresses TRPML1 with C-terminus mCherry tag in mammalian cells.

    Depositor

    Antony Galione

    Article

    Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

    Insert

    TRPML1 (Mcoln1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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40. gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

    Plasmid

    #217662

    Purpose

    expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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