Skip to main content
Addgene

We narrowed to 3,985 results for: LYC

Showing: 3751 - 3800 of 3985 results
  1. pVRC8400-SARS-CoV-2-RBD-K417N-E484K-N501Y-AVI

    Plasmid
    #176440
    Purpose
    Expresses SARS-CoV-2 RBD domain containing K417N-E484K-N501Y mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Insert
    SARS-CoV-2-RBD-K417N-E484K-N501Y (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    K417N, E484K, N501Y
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pVRC8400-SARS-CoV-2-S2P-AY.1-AVI

    Plasmid
    #176335
    Purpose
    Expresses SARS-CoV-2 S2P protein from the AY.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Insert
    SARS-CoV-2-S2P-AY.1 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T19R, T95I, G142D, del156-157, R158G, W258L, K417…
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pVRC8400-SARS-CoV-2-S2P-B.1.526-S477N-AVI

    Plasmid
    #176329
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.526 variant containing S477N mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Insert
    SARS-CoV-2-S2P-B.1.429 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T95I, D253G, S477N, D614G, A701V
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pVRC8400-SARS-CoV-2-S2P-B.1.526-E484K-AVI

    Plasmid
    #176330
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.526 variant containing E484K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Insert
    SARS-CoV-2-S2P-B.1.429 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T95I, D253G, E484K, D614G, A701V
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pVRC8400-SARS-CoV-2-S2P-B.1.617.1-AVI

    Plasmid
    #176333
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.617.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Insert
    SARS-CoV-2-S2P-B.1.617.1 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T95I, G142D, E154K, L452R, E484Q, D614G, P681R, Q…
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pαH-S-GSAS-B.1.351.v4

    Plasmid
    #173788
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 4
    Depositor
    Insert
    Spike (S-GSAS-B.1.351 variant version 4) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. Spike Display_Part 5 Spacer

    Plasmid
    #172733
    Purpose
    Encodes Part 5 of HexaPro-D614G spike to be used in the Spike Display system
    Depositor
    Insert
    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    Available since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  8. Spike Display_Part 4 Spacer

    Plasmid
    #172732
    Purpose
    Encodes Part 4 of HexaPro-D614G spike to be used in the Spike Display system
    Depositor
    Insert
    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    Available since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  9. Spike Display_Part 3 Spacer

    Plasmid
    #172731
    Purpose
    Encodes Part 3 of HexaPro-D614G spike to be used in the Spike Display system
    Depositor
    Insert
    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    Available since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  10. Spike Display_Part 1 Spacer

    Plasmid
    #172729
    Purpose
    Encodes Part 1 of HexaPro-D614G spike to be used in the Spike Display system
    Depositor
    Insert
    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    Available since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  11. Spike Display_Part 1b Spacer

    Plasmid
    #172728
    Purpose
    Encodes Part 1b of HexaPro-D614G spike to be used in the Spike Display system
    Depositor
    Insert
    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    Available since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  12. Spike Display_Part 1a Spacer

    Plasmid
    #172727
    Purpose
    Encodes Part 1a of HexaPro-D614G spike to be used in the Spike Display system
    Depositor
    Insert
    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    Available since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  13. Spike Display_Part 1-5 DO

    Plasmid
    #172726
    Purpose
    Encodes a sfGFP dropout expression cassette in place of Parts 1-5 to be used in the Spike Display system
    Depositor
    Insert
    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
  14. B9-COMP-blac-flag-his

    Plasmid
    #111020
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Insert
    6-cysteine protein (B9) (PF3D7_0317100 )
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
  15. PF3D7_1350600-COMP-blac-flag-his

    Plasmid
    #110968
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Insert
    PF3D7_1350600 (PF3D7_1350600 )
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  16. GPR180-COMP-blac-flag-his

    Plasmid
    #110955
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Insert
    PF3D7_1213500 (PF3D7_1213500 )
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Sept. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
  17. pFUSEss-CHIg-mM-Asp212Ser_M18

    Plasmid
    #91739
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asp212Ser (EU numbering) - N-glycosylated Asn209
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asp212 changed to Ser (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
  18. SARS-CoV-2 S HexaPro

    Plasmid
    #154754
    Purpose
    Mammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variant
    Depositor
    Insert
    Spike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    Available since
    July 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  19. GRP94-COMP-blac-flag-his

    Plasmid
    #111008
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Insert
    endoplasmin, putative (GRP94) (PFL1070c Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
  20. pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

    Plasmid
    #103871
    Purpose
    dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
    Depositor
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
  21. pAd-M3cherry

    Plasmid
    #61041
    Purpose
    This plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.
    Depositor
    Inserts
    Ngn3 (Neurog3 Mouse)
    pdx1 (Pdx1 Mouse)
    Mafa (Mafa Mouse)
    monomeric cherry
    Use
    Adenoviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  22. pTwist-SARS-CoV-2 Δ18 B.1.617.2v1

    Plasmid
    #179905
    Purpose
    Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
    Depositor
    Insert
    SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…
    Promoter
    CAG
    Available since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  23. pCRISPaint-mNeon-F-mgp100-PuroR [M1G]

    Plasmid
    #171802
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Insert
    mNeonGreen-FLAG-mgp100-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
  24. pαH-S-GSAS-OMICRON.BA.2-RBD

    Plasmid
    #184830
    Purpose
    Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Insert
    S-GSAS-OMICRON.BA2-RBD (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    6X His tag
    Expression
    Mammalian
    Mutation
    SARS-CoV-2 Spike RBD protein of OMICRON.BA.2 vari…
    Promoter
    Available since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  25. GAP50-COMP-blac-flag-his

    Plasmid
    #110956
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Insert
    secreted acid phosphatase (GAP50) (PF3D7_0918000 Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Sept. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
  26. MAEBL-COMP-blac-flag-his

    Plasmid
    #110963
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Insert
    merozoite adhesive erythrocytic binding protein (MAEBL) (PF3D7_1147800.1 Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Sept. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
  27. pαH-S-GSAS-OMICRON.BA.1-RBD

    Plasmid
    #184831
    Purpose
    Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.1 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Insert
    S-GSAS-OMICRON.BA.1 (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    6X His tag
    Expression
    Mammalian
    Mutation
    SARS-CoV-2 Spike RBD protein of OMICRON.BA.1 vari…
    Promoter
    Available since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  28. Spike Display_B.1.1.7

    Plasmid
    #172735
    Purpose
    Expression vector for SARS-CoV-2 (B.1.1.7) spike used in the Spike Display system
    Depositor
    Insert
    SARS-CoV-2 S B.1.1.7 (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 3751 - 3800 of 3985 results