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We narrowed to 3,245 results for: Streptococcus

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Showing: 1501 - 1520 of 3245 results

  1. p426_Cas9_gRNA-ARS1414a

    Plasmid
    #87400
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1414a sequence GCGCCACAGTTTCAAGGGTC in yeast chromosome 14.
    Depositor
    Aindrila Mukhopadhyay
    Publication
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1414a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87400-plasmid-map-sequence-id-508307

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  2. pTBL235 NheI-Cas9-KpnI-GSAlinker-FseI-TdT-NotI

    Plasmid
    #126480
    Purpose
    Expresses Cas9-GSAlinker-TdT in mammalian cells.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    GSA linker
    TdT (DNTT Human)
    Expression
    Mammalian
    Mutation
    Changed codons to reduce repetitive sequences.
    Promoter
    CMV
    Available since
    June 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    126480-plasmid-map-sequence-id-246056

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  4. PB-PE SpRY

    Plasmid
    #179082
    Purpose
    NOTE: An improved version of this plasmid is now available. See Addgene plasmid #235998. All-in-one prime editor piggyBac transposon, SpRY variant
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    A61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…
    Promoter
    CAG
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179082-plasmid-map-sequence-id-352656

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  6. pPC1000

    Plasmid
    #174829
    Purpose
    Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Inserts
    CRISPRi SpCas9 sgRNA cassette
    PuroR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    EF1a and mouse U6
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174829-plasmid-map-sequence-id-343648

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  7. MSP2253

    Plasmid
    #70704
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70704-plasmid-map-sequence-id-127764

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  9. eSpCas9(1.1)_No_FLAG_ATP1A1-G7

    Plasmid
    #173203
    Purpose
    Expresses the ATP1A1 G7 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 17. pX330-like plasmid.
    Depositor
    Yannick Doyon
    Publication
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G7 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173203-plasmid-map-sequence-id-339516

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  11. Level 1 P2 OsUbiP:Cas9:NosT

    Plasmid
    #165424
    Purpose
    GoldenGate (MoClo) Level 1 Position 2 OsUbiP: Cas9: NosT cassette
    Depositor
    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing
    Publication
    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.
    Insert
    Os Ubiquitin promoter :: Cas9 :: NosTerminator
    Use
    Synthetic Biology
    Promoter
    Os Ubiquitin promoter :: Cas9 :: NosTerminator
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    165424-plasmid-map-sequence-id-331265

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  13. pGL3-sgACSL3.C-Cas9-P2A-Puro

    Plasmid
    #129412
    Purpose
    Encoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminus
    Depositor
    Elina Ikonen
    Publication
    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 and sgACSL3.C (ACSL3 Human)
    Use
    CRISPR
    Tags
    P2A-Puro
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129412-plasmid-map-sequence-id-254823

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  14. pTBL210

    Plasmid
    #126451
    Purpose
    Expresses Cas9-XTEN-TdT in mammalian cells.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-XTEN-TdT (DNTT Human, Streptococcus pyogenes)
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    CMV
    Available since
    June 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    126451-plasmid-map-sequence-id-245970

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  15. PE2_lenti

    Plasmid
    #237895
    Purpose
    Prime Editor in lentiviral backbone
    Depositor
    Michael Boettcher
    Insert
    PE2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a
    Available since
    Sept. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    237895-plasmid-map-sequence-id-477301

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  16. ABE8e-NRCH

    Plasmid
    #257825
    Purpose
    ABE8e Sp Cas9 NRCH IVT plasmid
    Depositor
    Gregory Newby
    Insert
    ABE8e SpCas9 NRCH
    Expression
    Mammalian
    Available since
    June 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    257825-plasmid-map-sequence-id-513599

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Showing: 1501 - 1520 of 3245 results