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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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163,580 results

Showing: 1521 - 1540 of 163580 results

  1. rAAV2/MG1.2

    Plasmid
    #184541
    Purpose
    AAV packaging plasmid, expressing Rep2 and AAV-MG1.2 capsid
    Depositor
    Minmin Luo
    Article
    Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s
    Insert
    AAV-MG1.2 capsid
    Use
    AAV; Helper vector for packaging
    Tags
    Expression
    Mutation
    changes on AAV9 capsid: K449R, Q588T; insertion o…
    Promoter
    Available Since
    July 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    184541-plasmid-map-sequence-id-361941

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  5. mEGFP-NbALFA

    Plasmid
    #159986
    Purpose
    For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)
    Depositor
    Motoyuki Hattori
    Article
    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:
    Insert
    mEGFP-NbALFA
    Use
    Tags
    8histidine-HRV3C protease cleavage site
    Expression
    Bacterial
    Mutation
    3GS linker between mEGFP and NbALFA
    Promoter
    Available Since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    159986-plasmid-map-sequence-id-314744

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  7. EGFP-CAAX

    Plasmid
    #86056
    Purpose
    A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    Homo sapiens Harvey rat sarcoma (HRAS Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains the region from 825-857 of HRAS as a far…
    Promoter
    CMV
    Available Since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86056-plasmid-map-sequence-id-177924

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  9. pOpen-DpnIR

    Plasmid
    #165529
    Purpose
    Used to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    DpnI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165529-plasmid-map-sequence-id-339077

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  10. pTP1122

    Plasmid
    #104159
    Purpose
    Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
    Use
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104159-plasmid-map-sequence-id-252709

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  18. pDG02583

    Plasmid
    #104129
    Purpose
    Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    NEDD8-specific protease 1 (NEDP1)
    Use
    Tags
    14x Histidine tag, Maltose binding protein, and S…
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104129-plasmid-map-sequence-id-313426

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Showing: 1521 - 1540 of 163580 results