159,117 results

1. pLS-SV40-mP-EGFP

   Plasmid

   #137724

   Purpose

   Lentivirus based EGFP expression vector

   Depositor

   Nadav Ahituv

   Article

   Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

   Insert

   SV40 enhancer
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-GFP-HRP-TM_pLX304

   Plasmid

   #202021

   Purpose

   expresses GFP-HRP anchored to the extracellular side of the plasma membrane, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   Flag-GFP-HRP-TM
   

   Use

   Lentiviral

   Tags

   Flag and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-AAV-syn-iGABASnFR2-WPRE

   Plasmid

   #218874

   Purpose

   AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Has Service

   AAV1 and AAV5

   Insert

   iGABASnFR2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S99A F102Y F104Y L178S

   Promoter

   Synapsin

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCI-His-hUbi

   Plasmid

   #31815

   Depositor

   Astar Winoto

   Article

   Young et al J Biol Chem. 2011 Feb 25;286(8):6521-31.

   Insert

   Ubiquitin (UBB Human)
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9(BB)-2A-Blast

   Plasmid

   #118055

   Purpose

   Used for expression of sgRNA in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. CYBL-SPI1-CEBPB-IRF5

   Plasmid

   #190719

   Purpose

   For microglia differentiation from iPSC

   Depositor

   Li Gan

   Article

   Drager et al Nat Neurosci. 2022 Aug 11. pii: 10.1038/

   Insert

   SPI1-CEBPB-IRF5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. JD633

   Plasmid

   #160393

   Purpose

   CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning

   Depositor

   Jorge Dubcovsky

   Article

   Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

   Inserts

   ZmUbi::wheat GRF4-GIF1 chimera
   ZmUbi:TaCas9 + TaU6:gRNA
   

   Use

   Binary vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1GFP/RNase H1 WT

   Plasmid

   #174447

   Purpose

   Express GFP-tagged RNase H1 WT in E. coli.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   RNase H1 (RNASEH1 Human)
   

   Use

   Tags

   His-tag, GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-SIRT1

   Plasmid

   #1791

   Purpose

   Mammalian expression of flag-tagged SIRT1

   Depositor

   Michael Greenberg

   Article

   Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

   Insert

   SIRT1 (SIRT1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF3550

    Plasmid

    #145026

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-Pol-II WT

    Plasmid

    #35175

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. Stat3 pcDNA3

    Plasmid

    #8706

    Purpose

    See Depositor Comments below for variant information.

    Depositor

    Jim Darnell

    Article

    Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

    Insert

    Stat3 (Stat3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    * see comments

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.TBG.PI.eGFP.WPRE.bGH

    Plasmid

    #105535

    Purpose

    AAV expression of EGFP from TBG promoter

    Depositor

    James M. Wilson

    Has Service

    AAV8

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TBG

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-Kif2c

    Plasmid

    #208651

    Purpose

    Mammalian expression plasmid expressing eGFP-Kif2c driven by CMV promoter

    Depositor

    Samuel Bakhoum

    Article

    Li et al Nature. 2023 Aug;620(7976):1080-1088. do

    Insert

    Kif2c (KIF2C Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Factor IX donor

    Plasmid

    #182141

    Purpose

    Factor IX donor for twinPE and Bxb1 mediated insertion at ALB

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attP-Factor IX intron 1 and exon 2-8 CDS and exon 8 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTW2154

    Plasmid

    #236578

    Purpose

    ISYmu1 CHLl1 gRNA4 TRV2 Architecture_B

    Depositor

    Steven Jacobsen

    Article

    Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

    Insert

    ISYmu1 TnpB
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Flag-HA-USP22

    Plasmid

    #22575

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP22 (USP22 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHCMV-AmphoEnv

    Plasmid

    #15799

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    4070A Amphotropic Murine leukemia virus envelope gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. SP-dCas9-VPR

    Plasmid

    #63798

    Purpose

    SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    SP-dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR_PGK_LaG16_2_synNotch_Gal4VP64

    Plasmid

    #85421

    Purpose

    Lentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic Notch

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    LaG16_2 GFP nanobody synNotch Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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