We narrowed to 4,337 results for: Pet2;

1. WT C-His Ana GvpC in pET28a

   Plasmid

   #85732

   Purpose

   Expresses His-tagged GvpC from Anabaena flos-aquae in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

   Insert

   Gas vesicle protein C (his-tagged)
   

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Added an extra glycine after the methionine at th…

   Promoter

   T7

   Available Since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-SENP2 (catalytic domain)

   Plasmid

   #16357

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP2 catalytic domain (SENP2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acid 365 to 590 catalytic domain of human S…

   Available Since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-His6-GFP-C-LACE

   Plasmid

   #133913

   Purpose

   Bacterial expression of His6-GFP-C-LACE

   Depositor

   Jeffrey Bode

   Article

   Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

   Insert

   superfolder green fluorescent protein
   

   Tags

   LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21 Catalytic Domain of MALT1

   Plasmid

   #48970

   Purpose

   Expresses catalytic domain of MALT1 in e.coli AA 329-566

   Depositor

   Guy Salvesen

   Article

   Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

   Insert

   MALT1 (MALT1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains AA 329-566

   Promoter

   T7

   Available Since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pET23b-2E2-HA-mEGFP-6xHis

   Plasmid

   #129594

   Purpose

   The encoded protein is the anti-HA frankenbody variant fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

   Depositor

   Tim Stasevich

   Article

   Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

   Insert

   Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
   

   Tags

   6xHis-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-6His-SUMO-Nsp10

   Plasmid

   #154959

   Purpose

   Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

   Depositor

   Justin Kollman

   Insert

   6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
   

   Tags

   6His-Thrombin-SUMO

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pET28_mouse-Caspase-1_enzymatic-domain

   Plasmid

   #183385

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1 (AA130-404) (Casp1 Mouse)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a SUMO Nup53xl 1-320

   Plasmid

   #85998

   Purpose

   bacterial expression of his6 and SUMO tagged Xenopus laevis Nup53

   Depositor

   Wolfram Antonin

   Article

   Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

   Insert

   Xenopus laevis Nup53 (nup35.L Frog)
   

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   codon optimzed for bacterial expression

   Promoter

   T7

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-His6-Ran(Q69L)

   Plasmid

   #171563

   Purpose

   Encodes human Ran protein

   Depositor

   Yan Zeng

   Insert

   Ran (RAN Human)
   

   Expression

   Bacterial

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28sumo-MDA5-Full-length

    Plasmid

    #210826

    Purpose

    protein expression in ecoli

    Depositor

    Anna Pyle

    Article

    Fitzgerald et al Nucleic Acids Res. 2014 Apr;42(6):3919-3

    Insert

    MDA5 (IFIH1) Full Length Protein (IFIH1 Human)
    

    Expression

    Bacterial

    Mutation

    NA

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28_human-Caspase-4_enzymatic-domain

    Plasmid

    #183381

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-4 (AA94-377) (CASP4 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28b-ptetO::bac::gfp

    Plasmid

    #217882

    Purpose

    expresses the bac-BGC in bacterial cells, produces bacillamide D

    Depositor

    Tobias A. M. Gulder

    Article

    Hohmann et al Commun Biol. 2024 May 28;7(1):655. doi:

    Inserts

    Dehydrogenase
    Non-ribosomal peptide synthetase
    Aminotransferase
    

    Expression

    Bacterial

    Promoter

    ptetO

    Available Since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET22b-T5-sfGFP*- MmH-MH4R

    Plasmid

    #196409

    Purpose

    Express sfGFP with TAG and enzymes for biosynthesizing DOPA

    Depositor

    Han Xiao

    Article

    Chen et al J Mol Biol. 2021 Dec 20:167412. doi: 10.

    Inserts

    sfGFP with TAG at 151 position
    Tyrosine Hydroxylase (Th Mouse)
    dihydropterine reductase from human (QDPR Human)
    pterin-4a-carbinolamine dehydratase from human
    

    Expression

    Bacterial

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-SnoopTag-AffiHER2-SpyTag

    Plasmid

    #216280

    Purpose

    Expresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expression

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SnoopTag-AffiHER2-SpyTag
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6, SnoopTag, SpyTag, and Thrombin cleavage sit…

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-His10Smt3-gamma-toxin

    Plasmid

    #197000

    Purpose

    Bacterial expression plasmid for gamma toxin tRNA endonuclease

    Depositor

    Dragony Fu

    Article

    Lentini et al RNA. 2018 May;24(5):749-758. doi: 10.126

    Insert

    Gamma toxin
    

    Tags

    His10-Smt3

    Expression

    Bacterial

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET20b-NLuc-MBP-6xHis

    Plasmid

    #135931

    Purpose

    Expresses NanoLuciferase in bacterial cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    NanoLuciferase
    

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET22b-T5-sfGFP*-XcP4H-MH4R

    Plasmid

    #178043

    Purpose

    Express green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTP

    Depositor

    Han Xiao

    Article

    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

    Inserts

    superfolder green fluorescent protein with TAG at 151 position
    XcP4H with W179F mutation
    dihydropterine reductase from human
    pterin-4a-carbinolamine dehydratase from human
    

    Tags

    His tag

    Expression

    Bacterial

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-mOct4 Full length(FL)

    Plasmid

    #206394

    Purpose

    Expresses Oct4 with a 6xHis tag for protein purification

    Depositor

    Ralf Jauch

    Insert

    Oct4 (Pou5f1 Mouse)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-6His-TEV-Nsp14

    Plasmid

    #154954

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pET28-sfGFP-DogTag Loop A

    Plasmid

    #171779

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-DogTag Loop A
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag and linkers inserted between residues Val2…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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