We narrowed to 16,108 results for: nans

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pS6-TGFB2-STREP

Plasmid

#128503

Purpose

Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t STREP(2x) tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

TGFB2 (TGFB2 Human)

Tags

STREP (2X) tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pIE-C-hα2M-H6

Plasmid

#128494

Purpose

Expresses human C-α2M (from R787 to N1473) in insect cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (R787-N1473) (A2M Human)

Tags

His tag (6x)

Expression

Insect

Mutation

contains R787-N1473

Promoter

IE1

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pmNeon Green-N1-ARF1N/3C-R

Plasmid

#202433

Purpose

Mammalian expression vector containing mNeonGreen-tagged ARF3 C-terminus/ARF1 N-terminus chimera

Depositor

David Bryant

Article

Sandilands et al J Cell Biol. 2023 Apr 3;222(4):e20220611

Insert

ARF1 (ARF1 Human)

Use

Lentiviral

Tags

mNeonGreen

Mutation

C terminus replaced with ARF3 sequence (S174A/R17…

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAW-BiP-Nb127D01-mCherry-KDEL

Plasmid

#171575

Purpose

Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

BiP-Nb127D01-mCherry-KDEL (CXCR2 Human)

Tags

BiP signal peptide and mCherry-KDEL

Expression

Insect

Promoter

fly actin5C promoter

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAW-BiP-NbVHH05-mCherry-KDEL

Plasmid

#171574

Purpose

Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

BiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)

Tags

BiP signal peptide and mCherry-KDEL

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCri-H6-TEV-GRAB

Plasmid

#128498

Purpose

Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

GRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)

Tags

His tag (6x)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCri-H6-TEV-GRAB-STREP

Plasmid

#128499

Purpose

Expresses GRAB (from V34 to N181) in bacterial cells. With N-t H6x tag + TEV + protein + STREP(2x) tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

GRAB (V34-N181) (grab Streptococcus pyogenes serotype M1)

Tags

His tag (6x) and Strep tag (2x)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pUltra-Hot-PC

Plasmid

#184466

Purpose

Lentiviral vector for bi-cistronic expression of mCherry and PC (seperated by P2A)

Depositor

Gerardo Ferbeyre

Article

Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

Insert

Pyruvate Carboxylase (PC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

UbC

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-eHGT_78h-Cre_PEST

Plasmid

#231791

Purpose

Expresses Cre in excitatory neurons; Cre expression is attenuated by PEST sequence.

Depositor

Karl Deisseroth

Article

Drexler et al Cell. 2025 Aug 21;188(17):4640-4657.e30.

Insert

Cre

Use

AAV

Tags

PEST

Promoter

minBetaGlobin

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Blast-DNTGFBR2-HA

Plasmid

#130888

Purpose

Expresses dominant negative mutant human TGFBR2

Depositor

Gian-Paolo Dotto

Article

Bordignon et al Cell Rep. 2019 Aug 27;28(9):2358-2372.e6

Insert

TGFBR2 (TGFBR2 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

Dominant Negative receptor lacking cytosolic cata…

Promoter

CMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-neomycin-PC-MYC

Plasmid

#184550

Purpose

Retroviral vector to express human PC with MYC tag

Depositor

Gerardo Ferbeyre

Article

Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

Insert

Pyruvate Carboxylase (PC Human)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Promoter

LTR

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pET-26b-Nb127D01-ALFA-His

Plasmid

#171568

Purpose

Bacterial expression of ALFA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-ALFA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

Nb127D01-ALFA-His (CXCR2 Human)

Tags

ALFA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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cryS96

Plasmid

#67519

Purpose

αB-crystallin minipeptide fused to ELP (S96).

Depositor

Andrew MacKay

Article

Wang et al J Control Release. 2014 Oct 10;191:4-14.

Insert

mini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Synthetic, Human)

Tags

elastin-like polypeptide (ELP) S96

Expression

Bacterial

Mutation

mini-αB-crystallin contains residues 73–92 of aB-…

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
sgALK(A)

Plasmid

#85562

Purpose

Expresses sgRNA targeting human ALK around codon F1174

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

anaplastic lymphoma receptor tyrosine kinase (ALK Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
sgALK(B)

Plasmid

#85563

Purpose

Expresses sgRNA targeting human ALK around codon F1174

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

anaplastic lymphoma receptor tyrosine kinase (ALK Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pMT-HGP-v3-NbVHH05-hIgG

Plasmid

#171565

Purpose

Expresses hIgG-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-human IgG-TEV-Avi-His) in fly cells

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-human IgG-TEV-Avi-His (UBE2J1 Human)

Tags

BiP signal peptide and hIgG1-hinge-N297A-TEV site…

Expression

Insect

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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PB-iRFP-STOP-ReNL

Plasmid

#113965

Purpose

Piggybac transposon plasmid CRISPR gene deletion activatable fluorescence. Constitutive iRFP670 under EF1A promoter, CMV promoter with two SV40 polyA followed by red-enhanced nanolantern (ReNL)

Depositor

Jordan Green

Article

Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

Inserts

iRFP670
ReNL

Use

Piggybac transposon

Expression

Mammalian

Promoter

CMV - SV40-PolyA - SV40-PolyA and EF1A

Available Since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pET-TP1107(Q15TAG)

Plasmid

#247462

Purpose

Bacterial expression of anti-IgG VHH clone TP1107 with amber stop codon at Q15 for unnatural amino acid incorporation

Depositor

Angus Johnston

Article

Chen et al Nat Nanotechnol. 2025 Sep;20(9):1273-128

Insert

TP1107 Q15TAG

Tags

His6 and TEV protease cleavage site

Expression

Bacterial

Mutation

Original Q15 codon (CAA) was modified to amber st…

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b-RfxCas13d-His

Plasmid

#141322

Purpose

Plasmid for bacterial expression and purification of RfxCas13d protein

Depositor

Ariel Bazzini, Miguel Angel Moreno-Mateos

Article

Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

Insert

Rfx-Cas13d

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pT3TS-RfxCas13d-HA

Plasmid

#141320

Purpose

Plasmid to carry out IVT of RfxCas13d (human codon-optimized)

Depositor

Ariel Bazzini, Miguel Angel Moreno-Mateos

Article

Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

Insert

Rfx-Cas13d

Use

CRISPR

Tags

HA

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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