We narrowed to 5,650 results for: pCas

1. pT3TS-spCas9 ABE-Umax

   Plasmid

   #222138

   Purpose

   Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9 (cas9 Synthetic)
   

   Use

   CRISPR; Zebrafish expression

   Tags

   bipartite NLS

   Expression

   Mutation

   Promoter

   T3

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-All-in-one-hCascade

   Plasmid

   #134919

   Purpose

   Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAGGS-N-TEV-Twin-Strep

   Plasmid

   #202526

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   TEV cleavable site and Twin-strep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSpCas9n(BB)-2A-Puro (PX462) V2.0

   Plasmid

   #62987

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG-PLE-IRES-mCherry

   Plasmid

   #45766

   Depositor

   Luke Lavis

   Article

   Tian et al Proc Natl Acad Sci U S A. 2012 Mar 27;10

   Insert

   esterase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-SpCas9-P2A-puro

   Plasmid

   #214358

   Purpose

   Expresses SpCas9 and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SpCas9 and puromycin resistance genes
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSpCas9(BB)-2A-Puro G3BP1-sgRNA

   Plasmid

   #196197

   Purpose

   Cas9 + gRNA expression to edit G3BP1 locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAGGS-SARS2-S-D614 (C-flag)

   Plasmid

   #156420

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

   Depositor

   Hyeryun Choe, Michael Farzan

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS CoV2)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-MCS-TurboID-3xFLAG

   Plasmid

   #186814

   Purpose

   CAG promoter-driven expression of BioID2-fused protein (C terminus)

   Depositor

   Masahito Ikawa

   Article

   Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

   Type

   Empty backbone

   Use

   Tags

   TurboID-3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-eCas9-GFP-U6-gRNA

    Plasmid

    #79145

    Purpose

    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells

    Depositor

    Jizhong Zou

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAX APP Swe/Ind

    Plasmid

    #30145

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    human APP 695 Swedish/Indiana mutation (APP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Swedish and Indiana; K595N, M596L and V642F

    Promoter

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAGGS-C-TEV-Twin-Strep

    Plasmid

    #202527

    Purpose

    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. spCas9-BlastR (pCBhCas9-BlastR)

    Plasmid

    #71489

    Purpose

    Expresses SpCas9 and Blasticidin resistance

    Depositor

    Richard Sherwood

    Article

    Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

    Insert

    spCas9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CBh

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG_PSD95.FingR-eGFP-CCR5TC

    Plasmid

    #46295

    Depositor

    Don Arnold

    Article

    Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

    Insert

    PSD95.FingR-eGFP-CCR5TC (DLG4 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pScalps eSpCas9_Zsgreen gRNA scaffold

    Plasmid

    #188449

    Purpose

    Expression of eSpCas9 tagged with Zsgreen

    Depositor

    Ajit Chande

    Article

    Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1-alpha

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-Cre-IRES2-GFP

    Plasmid

    #26646

    Purpose

    Mammalian coexpression of nls-Cre and EGFP from the CAG promoter

    Depositor

    Anjen Chenn

    Article

    Woodhead et al J Neurosci. 2006 Nov 29;26(48):12620-30.

    Insert

    Cre
    

    Use

    Cre/Lox

    Tags

    GFP and IRES2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV pCAG-mRuby3-WPRE

    Plasmid

    #107744

    Purpose

    Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.

    Depositor

    Rylan Larsen

    Article

    Zhuang et al Cell Rep. 2021 Oct 12;37(2):109826. doi:

    Insert

    mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-3xFLAG-TurboID-MCS

    Plasmid

    #186813

    Purpose

    CAG promoter-driven expression of TurboID-fused protein (N terminus)

    Depositor

    Masahito Ikawa

    Article

    Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

    Type

    Empty backbone

    Use

    Tags

    3xFLAG-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSL103-SpCas9-FLAG-3XNLS

    Plasmid

    #182032

    Purpose

    Expression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP

    Depositor

    Steven Lin

    Article

    Lin et al Bio Protoc. 2022 May 20;12(10):e4420. do

    Insert

    Streptococcus pyogenes cas9 (cas9 Synthetic)
    

    Use

    CRISPR

    Tags

    12X His tag, 3XNLS, FLAG, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG RpH-LAMP1-3xFLAG

    Plasmid

    #163018

    Purpose

    expresses ratiometric sensor of lysosomal lumenal pH

    Depositor

    Massimiliano Stagi

    Article

    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

    Insert

    RpH-LAMP1-3xFLAG (Lamp1 Mouse)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge