172,049 results

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mTagRFP-T-H2B-6

Plasmid

#58019

Purpose

Localization: Nucleus/Histones, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

H2B (H2BC11 Human)

Tags

mTagRFP

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pGMCtKq28-TSC10M1-sspB

Plasmid

#49526

Purpose

Kanamycin resistant; contains qtag28, expresses sspB under a promoter containing PtetO, and constutively expresses single-chain TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety site

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

TSC10M1-sspB

Expression

Bacterial

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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EIF5A

Plasmid

#25260

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

eIF5A:S15-A151 (EIF5A Human)

Tags

His

Expression

Bacterial

Mutation

Contains amino acids S15-A151

Available Since

Aug. 13, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pGWB543

Plasmid

#74877

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pET tdTomato LIC cloning vector (H6-tdTomato)

Plasmid

#86928

Purpose

pET LIC cloning vector for Biotin-His6-tev-yORF-tdTomato

Depositor

Chris Jeans

Type

Empty backbone

Tags

6xHis, AviTag (biotin), TEV cleavage site, and td…

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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V5-ERG-R367K

Plasmid

#118624

Purpose

Expresses V5 tagged ERG R367K (DNA binding mutant) protein

Depositor

William Hahn

Article

Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

Insert

ERG (R367K DNA binding mutant) (ERG Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

R367K

Promoter

EF1a

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pWPI-UPP1-3xFLAG

Plasmid

#201644

Purpose

Over-expression of 3xFLAG-tagged human UPP1

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

UPP1 (UPP1 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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IFNAR2_pCSdest

Plasmid

#53914

Purpose

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

IFNAR2 (IFNAR2 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pLenti_CMV:Flag-NLuc_SV40:PuroR

Plasmid

#183045

Purpose

Expresses FLAG-tagged NanoLuciferase (NLuc-only) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

NanoLuciferase

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b_RanT24N

Plasmid

#186271

Purpose

Expression vector of His-RanT24N in E.coli

Depositor

Masahito Ikawa

Article

Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

GTP-binding nuclear protein Ran (Ran Mouse)

Tags

His tagged and His-tagged at N terminal

Expression

Bacterial

Mutation

Changed T24 into N

Promoter

T7 promoter

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-NET-myc

Plasmid

#107458

Purpose

Mammalian expression plasmid for codon optimized hNET with internal MYC tag inserted in ECL2

Depositor

Erik Procko

Article

Young et al bioRxiv 2021.04.19.440442

Insert

Norepinephrine Transporter (NET) (SLC6A2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-AKT1

Plasmid

#116710

Purpose

Lentiviral expression of AKT1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AKT1 (AKT1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC11A2

Plasmid

#131884

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC11A2 (SLC11A2 Human)

Expression

Mammalian

Available Since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pTR47m4

Plasmid

#104068

Purpose

Luciferase Reporter for Formaldehyde Sensing

Depositor

David Liu

Article

Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

Inserts

xluxAB
frmR
frm Promoter m4

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Mutation

23r and 25th nucleotides in sequence mutated from…

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF6_P2A_Hygro_Barcode

Plasmid

#120492

Purpose

Barcoded lentiviral vector to express KLF6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF6 (KLF6 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE.puro/TO/Flag/tGFP-HuR.wt

Plasmid

#110349

Purpose

Doxycycline inducible mammalian retroviral expression of HuR (wild type) with FLAG and turboGFP tags

Depositor

Sandra Martha Gomes Dias

Insert

ELAV like RNA binding protein 1 (ELAVL1 Human)

Use

Retroviral

Tags

FLAG and tGFP

Expression

Mammalian

Promoter

gag

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Human p150,95

Plasmid

#8633

Depositor

Timothy Springer

Article

Corbi et al EMBO J 1987 Dec 20;6(13):4023-8.

Insert

Human p150,95 (ITGAX Human)

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pMH-HA/Joseph2-Multitagged Plasmid

Plasmid

#226726

Purpose

Validates the expression and detection of epitope-tagged proteins of interest in mammalian cells.

Depositor

Richard Cripps

Article

Robinson et al Biotechniques. 2024;76(7):299-309. doi:

Insert

pMH-HA/Joseph2

Tags

HA, Myc, GFP, Flag, V5, His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-H1-shSRGAP2B/C#2-hsynapsin-EGFP

Plasmid

#220797

Purpose

shRNA plasmid against human SRGAP2B/C genes (#2) with EGFP expression in neurons

Depositor

Pierre Vanderhaeghen

Article

Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

Insert

EGFP

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-hCD46-T2A-GFP

Plasmid

#205457

Purpose

lentiviral plasmid for expression of human CD46

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

hCD46 (CD46 Human)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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