We narrowed to 4,174 results for: pes

1. pET42b-BE3

   Plasmid

   #87437

   Purpose

   Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

   Insert

   BE3 (Apobec1 Human, Bacillus phage PBS2, Rat)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-MMLV-gag-hMLH1dn

   Plasmid

   #195513

   Purpose

   MMLV-Gag fused to hMLH1dn with TSTLLMENSS cleavage site between Gag-NES and hMLH1dn. For production of virus like particles with base-editor RNP cargo.

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   hMLH1dn (MLH1 Human)
   

   Use

   Virus like particle

   Tags

   FLAG, NES, and Protease cleavate site

   Expression

   Mammalian

   Mutation

   Human MLH1 del754-756

   Promoter

   CMV

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGMC00035 (aka pMG0784)

   Plasmid

   #210768

   Purpose

   Lentiviral vector to express human ADRM1

   Depositor

   Raj Chari

   Article

   Osei-Amponsa et al Mol Cell. 2024 Feb 1;84(3):522-537.e8. d

   Insert

   ADRM1 (ADRM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUW-TetO-Gata6

   Plasmid

   #128827

   Purpose

   dox-dependent expression of mouse Gata6

   Depositor

   Yossi Buganim

   Article

   Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

   Insert

   Gata6 (Gata6 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-30nmEML-mRFP

   Plasmid

   #231182

   Purpose

   Expresses a synthetic linker, tagged with mRFP, stabilizing the ER-mitochondrial distance at 30 nm from a lentiviral vector

   Depositor

   Dmitry Lim

   Article

   Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

   Insert

   30nmEML-mRFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET42b-HF-BE3

   Plasmid

   #87438

   Purpose

   Expresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

   Insert

   HF-BE3 (Apobec1 Human, Bacillus phage PBS2, Rat)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pR26-CMVconst-mBMP7

   Plasmid

   #127375

   Purpose

   Allows for constitutive BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

   Depositor

   Lance Miller

   Article

   Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

   Insert

   BMP7 (Bmp7 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CMV enhancer/promoter

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. RCAS-Flag-BRAFV600E

   Plasmid

   #126615

   Purpose

   Retroviral expression of Flag tagged human oncogenic BRAFV600E

   Depositor

   Yi Li

   Article

   Bu et al Cancer Res. 2019 Jan 1;79(1):61-71. doi:

   Insert

   BRAF (BRAF Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   V600E

   Promoter

   Available Since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAD29_hIRG1_4-461_pvp008

   Plasmid

   #124843

   Purpose

   Expresses human CAD (Irg1) in E.coli

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase (ACOD1 Human)
   

   Use

   Tags

   StrepTag and TEV site

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-3 and 462-end.

   Promoter

   T7

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-hSynI-rtTAV16

    Plasmid

    #102367

    Purpose

    AAV vector to drive TET-ON activator expression from human synapsin I promoter

    Depositor

    Tetsuo Yamamori

    Article

    Watakabe et al Front Neural Circuits. 2014 Sep 17;8:110

    Insert

    rtTAV16
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    rtTA variant reported in Gene Therapy (2006) 13, …

    Promoter

    human synapsin I promoter

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAGT9569

    Plasmid

    #219431

    Purpose

    Transcriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOS

    Depositor

    Tom Schreiber

    Article

    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

    Insert

    LB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)
    

    Use

    CRISPR and Synthetic Biology; Moclo compatible le…

    Tags

    PapE-4LF2

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCerulean3-Bcl-XL-s2193

    Plasmid

    #166749

    Purpose

    Express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL, in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.

    Depositor

    David Andrews

    Article

    Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

    Insert

    Bcl-XL (BCL2L1 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    Human ferritin

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-HF-BE3

    Plasmid

    #87439

    Purpose

    Expresses HF-BE3 with C-terminal NLS in mammalian cells

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

    Insert

    HF-BE3 (Apobec1 Human, Bacillus phage PBS2, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mammalian codon-optimized Cas9-HF

    Promoter

    CMV

    Available Since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSico-CAG-v-H-Ras-IRES-Luciferase/EGFP

    Plasmid

    #58959

    Purpose

    lentiviral expression of v-H-Ras and luciferase/EGFP reporter

    Depositor

    Snorri Thorgeirsson

    Article

    Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

    Inserts

    CMV enhancer/chicken beta actin promoter
    v-H-Ras
    luc2/EGFP fusion gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer/chicken beta actin

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUCmini-iCAP-PHP.V2

    Plasmid

    #127848

    Purpose

    non-standard AAV2 rep-AAV-PHP.V2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

    Insert

    Synthetic construct isolate AAV-PHP.V2 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. K84M kinase-dead Nuak1-3xflag

    Plasmid

    #97219

    Purpose

    Plasmid expressing kinase-dead mutant Nuak1

    Depositor

    Huda Zoghbi

    Article

    Lasagna-Reeves et al Neuron. 2016 Oct 19;92(2):407-418. doi:

    Insert

    Nuak1 (NUAK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Kinase-dead K84M

    Promoter

    T7

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUCmini-iCAP-PHP.B4

    Plasmid

    #127849

    Purpose

    non-standard AAV2 rep-AAV-PHP.B4 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

    Insert

    Synthetic construct isolate AAV-PHP.B4 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-DIO-FLPo-Myc

    Plasmid

    #124641

    Purpose

    AAV-mediated Cre-dependent expression of FLPo recombinase under EF1a promoter

    Depositor

    David Dupret

    Article

    Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

    Insert

    FLPo
    

    Use

    AAV and Cre/Lox

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAD39_mIRG1_4-462_pvp008

    Plasmid

    #124873

    Purpose

    Expresses mouse CAD (Irg1) in E.coli

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (Acod1 Mouse)
    

    Use

    Tags

    StrepTag and TEV site

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-3 and 463-end.

    Promoter

    T7

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1a-FDIO-ArchT-GFP

    Plasmid

    #124640

    Purpose

    AAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoter

    Depositor

    David Dupret

    Article

    Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

    Insert

    ArchT
    

    Use

    AAV; Flp/frt

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCAD104_Asn152Ser_pCMV6Entry-hIRG1

    Plasmid

    #124877

    Purpose

    Expresses hCAD Asp152Ser mutant in mammalian cells

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Use

    Tags

    myc and FLAG tags

    Expression

    Mammalian

    Mutation

    mutated Asn152 to Ser

    Promoter

    CMV

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCAD64_pCMV6_Entry_hIrg1

    Plasmid

    #124879

    Purpose

    Expresses hCAD His103Ala mutant in mammalian cells

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Use

    Tags

    myc and FLAG tags

    Expression

    Mammalian

    Mutation

    mutated His103 to Ala

    Promoter

    CMV

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pX330-Elf5 gRNA

    Plasmid

    #128836

    Purpose

    gRNA for targeting mouse Elf5 loci using CRISPR-cas technique

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    mElf5 gRNA (Elf5 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. H14-MBP-SUMO-FLAG-LEM

    Plasmid

    #101778

    Purpose

    Expresses His-MBP-tagged mutant LEM-domain (FLAG-tagged) in E.coli (SUMO-cleavable)

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    MAN1 (LEMD3 Human)
    

    Use

    Tags

    His14-MBP-SUMO-FLAG

    Expression

    Bacterial

    Mutation

    amino acids 6-51 of human MAN1 (LEM domain)

    Promoter

    T5

    Available Since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. FUW-TetO-Klf5

    Plasmid

    #128830

    Purpose

    dox-dependent expression of mouse Klf5

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    Klf5 (Klf5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-500-WPRE

    Plasmid

    #174134

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-500 fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

    Insert

    Nos1ap (Nos1ap Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Expression

    Mutation

    Only contains the sequences coding for amino acid…

    Promoter

    hSyn

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCAD54_CAD29_H104A_pvp008

    Plasmid

    #124876

    Purpose

    Expresses hCAD His103Ala mutant in E.coli

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Use

    Tags

    StrepTag and TEV site

    Expression

    Bacterial

    Mutation

    mutated His103 to Ala, deleted amino acids 1-3 an…

    Promoter

    T7

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. pNTKV- Esrrb-KI-3'UTR-2A-TagBFP-RBGpA

    Plasmid

    #128835

    Purpose

    Knock-in of TagBFP into mouse Esrrb locus

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    tagBFP into Esrrb locus (Esrrb Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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29. pAAV-hGRM1-RFP

    Plasmid

    #22920

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    human receptor metabotropic 1 promoter driving DsRedExpress (GRM1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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30. Gal4-VP16-HSF1 WT

    Plasmid

    #71726

    Purpose

    plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT RD

    Depositor

    Lea Sistonen

    Article

    Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

    Insert

    Gal4 DBD HSF1 VP16 AD (HSF1 Human, Budding Yeast, Herpes Simplex Virus protein VP16)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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