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Plasmid#173886PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-mRFP; gcry1: BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Control-cx43.4-48H-pGTag
Plasmid#117818PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsCAAXExpressionMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pPRISM-gal4-VP16-cmlc2-mRFP
Plasmid#117781PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertgal4/VP16
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGTag-NLS-TagRFP-SV40
Plasmid#117805PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsNLSExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1
Plasmid#198240PurposeFor I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sitesDepositorInsertsUseZebrafish transgenesisTagsExpressionMutationPromoterAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCK074 p5E exorh:EGFP
Plasmid#195951PurposeGateway p5E vector with reporterDepositorInsertexorh:EGFP
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tol2-cmlc2-Trpa1b-2A-EGFP
Plasmid#106405PurposeFor expressing Trpa1b-2A-EGFP in zebrafish cardiomyocytes.DepositorInsertsUseGatewayTags2A-EGFPExpressionMutationPromoterAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol2_claudink:Cas9green; erbb2_gRNA171
Plasmid#199339PurposepDEL142; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous erbb2 sgRNADepositorInsertsclaudink
Cas9
erbb2 sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
zCREST3:Cas9green; EGF_gRNA
Plasmid#199333PurposepDEL158; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 EGF sgRNADepositorInsertszCREST3
Cas9
nrg1 EGF sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2_NBT:Cas9green; erbb2_gRNA171
Plasmid#199337PurposepDEL134; transgenic construct to express cell-specific Cas9 in neurons; ubiquitous erbb2 sgRNADepositorInsertsNBT
Cas9
erbb2 sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2_claudink:Cas9green; control_gRNA
Plasmid#199336PurposepDEL143; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous control sgRNADepositorInsertsclaudink
Cas9
control sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-mRFP-; gcry1:BFP -1
Plasmid#173887PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-mRFP; gcry1: BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
zCD2
Plasmid#122031PurposeExpresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.DepositorInsertZebrafish HDAC6 Catalytic Domain 2
UseTagsHis6-MBP tag with cleavable TEV siteExpressionBacterialMutationPromoterT7Available sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_eZ3_Gal4VP64
Plasmid#169917PurposeExpression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.DepositorInsertantiCD19-Notch3-GL4VP64
UseLentiviralTagsExpressionMammalianMutationAdditional EGF repeat between the extracellular d…PromoterPGKAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP1-sox17 promoter
Plasmid#31400DepositorInsertsox17 promoter (sox17 Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCS2+Paxillin-mKate
Plasmid#105974Purposein vitro transcription, paxillin coding sequence without stop codon-paxilin is a protein of focal adhesionsDepositorInsertPaxillin
UseTagsmKateExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-acta1:QFGal4-SV40pA
Plasmid#155121PurposeTol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertacta1:QFGal4
UseZebrafish expressionTagsExpressionMutationPromoteracta1Available sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-lyzC
Plasmid#107591PurposeAn entry vector with a lyzC promoterDepositorInsertLysozyme C promoter
UseTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol1:uas-myc-act-map2k2b-2a-tdTomato
Plasmid#104721PurposeUAS-driven expression of activated MEKDepositorInsertactivated map2k2b (map2k2b Zebrafish)
UseZebrafish transposonTags2A-tdTomato and myc tagExpressionMutationserines 221 and 225 to aspartic acidPromoter10xUASAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only