2,159 results

1. p5E_unc503

   Plasmid

   #64020

   Purpose

   5' Gateway entry vector with muscle-specific promoter of zebrafish unc-45b

   Depositor

   Peter Currie, Joachim Berger

   Article

   Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

   Insert

   muscle-specific promoter of zebrafish unc-45b (unc45b Zebrafish)
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   503-bp promoter of zebrafish unc-45b

   Available since

   May 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. p5E-0.349cldn15la

   Plasmid

   #125026

   Purpose

   Five prime entry vector containing 349bp cldn15la promoter from zebrafish. Drives expression in intestinal epithelial cells.

   Depositor

   John Rawls

   Article

   Murdoch et al PLoS Pathog. 2019 Mar 7;15(3):e1007381.

   Insert

   Claudin 15-like a (cldn15la Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MiniCoopR mitfa:KIT K642E

   Plasmid

   #118849

   Purpose

   Expresses human KIT K642E mutant and zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Insert

   KIT K642E (KIT Zebrafish, Human)
   

   Use

   Zebrafish expression, tol5

   Tags

   Expression

   Mutation

   KIT K642E

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Gfp-zf-vasa

   Plasmid

   #167323

   Purpose

   Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.

   Depositor

   Goro Yoshizaki

   Article

   Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

   Insert

   zf vasa 3'UTRs (ddx4 Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCK011 p5E exorh

   Plasmid

   #195949

   Purpose

   Gateway p5E vector

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   exorh regulatory elements
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST mfap4:icre-p2a-tomato

   Plasmid

   #135217

   Purpose

   Cre coexpressed with tdTomato in macrophages

   Depositor

   David Tobin

   Article

   Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

   Insert

   mfap4 promoter with iCre and Tomato
   

   Use

   Cre/Lox; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2+ Zf GFP-vclb

   Plasmid

   #185411

   Purpose

   GFP tagged form of zebrafish vinculin b. For in vitro transcription (SP6) or expression through CMV

   Depositor

   Nicolas David

   Article

   Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

   Insert

   vinculin b (vclb Zebrafish)
   

   Use

   In vitro synthesis of mrna

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2+ Zf GFP-vcla

   Plasmid

   #185410

   Purpose

   GFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMV

   Depositor

   Nicolas David

   Article

   Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

   Insert

   vinculin a (vcla Zebrafish)
   

   Use

   In vitro synthesis of mrna

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGTag-TagRFP-CAAX-SV40

   Plasmid

   #117809

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   TagRFP
   

   Use

   Synthetic Biology

   Tags

   CAAX

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus

    Plasmid

    #105127

    Purpose

    Used along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent protein

    Depositor

    Kimberley Evason, Didier Stainier

    Article

    Evason et al PLoS Genet. 2015 Jul 2;11(7):e1005305. d

    Inserts

    Zebrafish fabp10a promoter:Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
    Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)
    

    Use

    Zebrafish transgenesis

    Tags

    Expression

    Mutation

    Promoter

    cryaa (crystallin) and fabp10a

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTol2_LSEC:Cas9green; erbb2_gRNA171

    Plasmid

    #199338

    Purpose

    pDEL135; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous erbb2 sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    LSEC
    Cas9
    erbb2 sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST_6xUAS:miRFP670-cetn4

    Plasmid

    #192357

    Purpose

    zfCETN4 tagged with miRFP670, expressed under the UAS promoter

    Depositor

    Darren Gilmour

    Article

    Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

    Insert

    CETN4 (cetn4 Zebrafish)
    

    Use

    Tags

    miRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. p5E-1.7kbfabp6

    Plasmid

    #159087

    Purpose

    p5E with promoter sequence 1.7kb upstream of zebrafish fabp6 gene

    Depositor

    John Rawls

    Article

    Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

    Insert

    1.7kb fabp6 promoter (fabp6 Zebrafish)
    

    Use

    Promoter fragment 5' entry vector for tol2 c…

    Tags

    Expression

    Mutation

    Promoter

    1.7kb fabp6

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. th-P2A-EGFP donor

    Plasmid

    #65562

    Purpose

    Intron targeting-mediated EGFP knockin at the zebrafish th locus.

    Depositor

    Jiu-lin Du

    Article

    Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

    Insert

    th-P2A-EGFP (th Zebrafish)
    

    Use

    Zebrafish injection

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6a:sgRNA(tyr)

    Plasmid

    #64250

    Purpose

    expresses sgRNA(tyr) under U6a promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Insert

    U6a:sgRNA (tyr)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. p5E-elavl3

    Plasmid

    #72640

    Purpose

    Gateway p5E 5'entry clone with elavl3 (HuC) enhancer/promoter for pan-neuronal expression in zebrafish

    Depositor

    Joshua Bonkowsky

    Insert

    elavl3 enhancer (elavl3 Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tol2-krt4-GFP

    Plasmid

    #97151

    Purpose

    label apical epithelial cells in zebrafish

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Insert

    AcGFP
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMT-sox10-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80063

    Purpose

    Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Sox10 promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPRISM-gal4-VP16-gcry-eGFP

    Plasmid

    #117779

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    gal4/VP16
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCK052 p5E exorh:mCherry

    Plasmid

    #195953

    Purpose

    Gateway p5E vector with reporter

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    exorh:mCherry
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge