2,162 results

1. pFRT-TODestRFP_RNAseH1

   Plasmid

   #65785

   Purpose

   expresses RFP-tagged RNAseH1 in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   RNaseH1 (RNASEH1 Human, Zebrafish)
   

   Use

   Tags

   RFP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available Since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Phat2-dr-cPla2-C2-Y90F-V91F-EGFP-6H

   Plasmid

   #187186

   Purpose

   Protein Expression

   Depositor

   Philipp Niethammer

   Article

   Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

   Insert

   ENSDARG00000024546 (pla2g4aa Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Y90F, V91F

   Promoter

   Available Since

   Aug. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-Rab13 in pCSDEST2

   Plasmid

   #140879

   Purpose

   Expression of zebrafish GFP-tagged Rab13 in mammalian cells.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Rab13 (rab13 Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. zCREST3:redCas9; control_gRNA

   Plasmid

   #199334

   Purpose

   pDEL157; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous control sgRNA

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Inserts

   zCREST3
   Cas9
   control sgRNA
   

   Use

   Tol2 destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tol2-lyzC-mito-Dendra2

   Plasmid

   #107590

   Purpose

   Expresses mitochondria localized Dendra2 in zebrafish neutrophils

   Depositor

   Qing Deng

   Article

   Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

   Insert

   mito-Dendra2
   

   Use

   Tags

   mitochondira localization sequence

   Expression

   Mutation

   Promoter

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDestTol2-actinb-kcna1a-IRES-EGFP

   Plasmid

   #164965

   Purpose

   Tol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFP

   Depositor

   GuangJun Zhang

   Article

   Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

   Insert

   kcna1a (kcna1a Zebrafish)
   

   Use

   Tol2 transposon destination vector

   Tags

   IRES-EGFP

   Expression

   Mutation

   Promoter

   actinb (actb2) promoter

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME Integrin beta 1b

   Plasmid

   #105976

   Purpose

   entry clone for gateway cloning, Full length Integrin beta 1b without stop

   Depositor

   Caren Norden

   Article

   Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

   Insert

   Integrinbeta1b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5E-dr-myl7-pro (JDW 1148)

   Plasmid

   #229836

   Purpose

   A gateway compatible 5' entry clone containing the mysoin light chain seven (myl7/cmlc2) promoter for cardiomyocyte specific expression.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   cmlc2/myl7
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFRT-TODestFLAGHA_RNAseH1

   Plasmid

   #65782

   Purpose

   expresses FLAGHA-tagged RNAsH1 in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   RNaseH1 (RNASEH1 Human, Zebrafish)
   

   Use

   Tags

   FLAG HA and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available Since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2

    Plasmid

    #218205

    Purpose

    Zebrafish -2.1prox1a enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector

    Depositor

    Katarzyna Koltowska

    Article

    Panara et al Development. 2024 May 1;151(9):dev202525

    Insert

    -2.1prox1a
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. 5UAS zfSynaptophysin:GFP

    Plasmid

    #74316

    Purpose

    A fusion of zebrafish synaptophysin to GFP under the control of 5UAS repeats

    Depositor

    Martin Meyer, Stephen Smith

    Article

    Meyer et al J Neurosci. 2006 Mar 29;26(13):3604-14.

    Insert

    synaptophysin
    

    Use

    Tags

    EGFP

    Expression

    Mutation

    Q12H and deletion of F13 (please see depositor co…

    Promoter

    5UAS

    Available Since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEM-NLS-Cre-2A-mCherry-FKF

    Plasmid

    #119868

    Purpose

    Donor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

    Insert

    NLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSB-CMV-MCS-Puro cPLA2-mK2-P2A-EGFP-LaminB1

    Plasmid

    #162566

    Purpose

    Used for the dual color labeling of cPLA2 and LaminB1 expression.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

    Inserts

    cytosolic phospholipase a2 (pla2g4aa Zebrafish)
    Lamin B1 (lmnb1 Zebrafish)
    

    Use

    Tags

    EGFP, P2A, and mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SV40

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p5E mpeg1

    Plasmid

    #135199

    Purpose

    Macrophage specific label

    Depositor

    David Tobin

    Article

    Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

    Insert

    mpeg1 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    mpeg1

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCK036 desmaMCS:minCerulean,cryaa:Venus

    Plasmid

    #195981

    Purpose

    Expression construct

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, cryaa Venus (desma Zebrafish)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2+-FLAG3-drAgo2DY-EF

    Plasmid

    #221716

    Purpose

    Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2DY-EF (ago2 Zebrafish)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    D651E and Y680F in active site

    Promoter

    simian CMV IE94

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2+-FLAG3-drAgo2

    Plasmid

    #221712

    Purpose

    Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2 (ago2 Zebrafish)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT2KXIG (Tol2)-tp63:ikk1KD:GFP

    Plasmid

    #105645

    Purpose

    Kinase-deleted Ikk1(alpha) expression in basal keratinocytes

    Depositor

    Sandra Rieger

    Article

    Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

    Insert

    tp63:ikk1KD:GFP (chuk Zebrafish)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    p63 promoter + ikk1 (kinase domain deleted) + GFP

    Promoter

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. p5E_unc503

    Plasmid

    #64020

    Purpose

    5' Gateway entry vector with muscle-specific promoter of zebrafish unc-45b

    Depositor

    Joachim Berger, Peter Currie

    Article

    Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

    Insert

    muscle-specific promoter of zebrafish unc-45b (unc45b Zebrafish)
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    503-bp promoter of zebrafish unc-45b

    Available Since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. p3e UbB polyA

    Plasmid

    #188702

    Purpose

    Gateway 3' element encoding the ubiquitin B polyadenylation sequence

    Depositor

    David Tobin

    Article

    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

    Insert

    UbB polyA (ubb Zebrafish)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge