762 results

1. mTFP1-C-Src-7

   Plasmid

   #55472

   Purpose

   Localization: Membrane/Vesicles, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   C-Src
   

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMK277 (mClover-NeoR)

   Plasmid

   #72793

   Purpose

   C-terminal tagging with mClover using CRIPSR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mClover-Neo
   

   Use

   CRISPR

   Tags

   mClover

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pmCherry-Vinculin

   Plasmid

   #80024

   Purpose

   Mammalian expression of mCherry-vinculin

   Depositor

   Chinten James Lim

   Article

   Lee et al Mol Biol Cell. 2013 May;24(9):1354-62. d

   Insert

   vinculin (VCL Chicken)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

4. 2XDR4

   Plasmid

   #32615

   Depositor

   Connie Cepko

   Article

   Billings et al PLoS One. 2010 . 5(10):e13739.

   Insert

   2XDR4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mTagRFP-T-C-Src-7

   Plasmid

   #58006

   Purpose

   Localization: Membrane/Vesicles, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Insert

   C-Src
   

   Use

   Tags

   mTagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. AP-SEMA3A (chick)

   Plasmid

   #29448

   Depositor

   Jonathan Raper

   Article

   Feiner et al Neuron. 1997 Sep . 19(3):539-45.

   Insert

   AP-SEMA3A (chick) (SEMA3A Chicken)
   

   Use

   Tags

   AP tagged

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pLNCX chick src

   Plasmid

   #13665

   Depositor

   Joan Brugge

   Insert

   Src (SRC Chicken)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

   Plasmid

   #68720

   Purpose

   Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

   Depositor

   Mark Huebener, Tobias Bonhoeffer, Tobias Rose

   Article

   Rose et al Science. 2016 Jun 10;352(6291):1319-22.

   Has Service

   AAV1

   Insert

   mRuby2-P2A-GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Cre-dependent expression from inverted open readi…

   Promoter

   hSyn1-FLEX

   Available since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10

   Plasmid

   #218867

   Purpose

   LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

   Insert

   RSET-jGCaMP8f
   

   Use

   Tags

   6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

   Expression

   Insect

   Mutation

   Q315L

   Promoter

   hsp70

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. aTRE

    Plasmid

    #32612

    Depositor

    Connie Cepko

    Article

    Billings et al PLoS One. 2010 . 5(10):e13739.

    Insert

    Thyroid Response Element
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET30b jGCaMP8f F366H

    Plasmid

    #204139

    Purpose

    Production of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coli

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f F366H
    

    Use

    Tags

    N-terminal His tag

    Expression

    Bacterial

    Mutation

    Phenylalanine 366 changed to histidine

    Promoter

    T7

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGP-CMV-GCaMP3-373

    Plasmid

    #91779

    Purpose

    Mammalian expression of low affinity calcium-sensing fluorescent protein

    Depositor

    Douglas Kim, GENIE Project

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    GCaMP3-373
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA

    Plasmid

    #51473

    Purpose

    Bicistronic vector expressing mRuby2 and GCaMP6m from a single open reading frame.

    Depositor

    Mark Huebener, Tobias Bonhoeffer, Tobias Rose

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Insert

    mRuby2-P2A-GCaMP6m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-Y-RAN-GFP26

    Plasmid

    #106423

    Purpose

    Y-RAN-GFP26 (RANbody GFP26::Chicken IgY)

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

    Insert

    Y-RAN-GFP26
    

    Use

    Tags

    HA and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCS2-FAK30

    Plasmid

    #59131

    Purpose

    Cloning vector for FAK fret sensor with 30 nm helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBS ColI alpha2

    Plasmid

    #19207

    Depositor

    Cliff Tabin

    Article

    Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

    Insert

    Alpha 2 type I collagen (COL1A2 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pZac2.1-GfaABC1D-lck-jGCaMP8m

    Plasmid

    #176760

    Purpose

    AAV-mediated expression of membrane bound GCaMP8m under the control of GfaABC1D promoter

    Depositor

    Loren Looger

    Has Service

    AAV1 and AAV9

    Insert

    lck-jGCaMP8m
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKH3-avian RSK1 K112/464R

    Plasmid

    #8981

    Depositor

    John Blenis

    Article

    Roux et al Mol Cell Biol. 2003 Jul . 23(14):4796-80

    Insert

    RSK (RPS6KA1 Chicken)
    

    Use

    Tags

    3x HA

    Expression

    Mammalian

    Mutation

    K112R, K464R

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pKH3-avian RSK1

    Plasmid

    #8998

    Depositor

    John Blenis

    Article

    Richards et al Mol Cell Biol. 2001 Nov . 21(21):7470-80

    Insert

    RSK (RPS6KA1 Chicken)
    

    Use

    Tags

    3x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pBS Galectin1

    Plasmid

    #19216

    Depositor

    Cliff Tabin

    Article

    Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

    Insert

    Galectin1 (LGALS1 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

21. pMK285 (Stag-3FLAG-Bsr)

    Plasmid

    #72801

    Purpose

    C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    Stag-3FLAG-Bsr
    

    Use

    CRISPR

    Tags

    Stag-3FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET15b/GgVcl 884-1066-ECFP

    Plasmid

    #46182

    Depositor

    Susan Craig

    Insert

    Vcl 884-1066 (VCL Chicken)
    

    Use

    Tags

    ECFP and His

    Expression

    Bacterial

    Mutation

    aa 884-1066 (tail domain)

    Promoter

    T7

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

23. mApple-Paxillin-22

    Plasmid

    #54935

    Purpose

    Localization: Focal Adhesions, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Thievessen et al J Cell Biol. 2013 Jul 8;202(1):163-77. d

    Insert

    Paxillin
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

24. VinTS-tCRMod-GGSGGS5

    Plasmid

    #111763

    Purpose

    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)5-mRuby2 module

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)5 extensible domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV-hSyn-Ribo-jGCaMP8f

    Plasmid

    #167573

    Purpose

    DEPRECATED: Please see Addgene item #169249 (coming soon) for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    Ribo-GCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GgVcl 1-1066 FRET tail probe 1

    Plasmid

    #46276

    Depositor

    Susan Craig

    Article

    Chen et al J Cell Biol. 2005 May 9;169(3):459-70.

    Insert

    Vcl 1-1066 + EYFP (VCL Chicken)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    addition of 9 bp linker and EYFP at aa 883

    Promoter

    CMV

    Available since

    July 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSNAP-CAPZ

    Plasmid

    #69948

    Purpose

    Simultaneous expression of a His- and SNAP-tagged β1 subunit and untagged α1 subunits of chicken CapZ

    Depositor

    Jeff Gelles

    Article

    Bombardier et al Nat Commun. 2015 Nov 13;6:8707. doi: 10.

    Inserts

    CapZ beta 1 (CAPZB Chicken)
    CapZ alpha 1 (CAPZA1 Chicken)
    

    Use

    Tags

    His 6 and SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PA-mCherry-miniSOG-Paxillin-22

    Plasmid

    #57796

    Purpose

    Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Paxillin
    

    Use

    Tags

    PA-mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pCS2-FAK0

    Plasmid

    #59128

    Purpose

    Cloning vector for FAK fret sensor without helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTK_Sox9_enh_349_Citrine

    Plasmid

    #130594

    Purpose

    enhancer reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    Sox9_enh_349
    

    Use

    Enhancer reporter

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge