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Showing: 281 - 320 of 4777 results

  2. pSNRW-Z5-Nluc

    Plasmid
    #174487
    Purpose
    Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.
    Depositor
    Richard C. Willson
    Article
    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1
    Insert
    Z5-Nluc
    Use
    Tags
    6x Histidine tag (N terminal on insert)
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac promoter
    Available since
    Oct. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    174487-plasmid-map-sequence-id-345291

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  9. pGW-Dronpa-Cav1.2

    Plasmid
    #182333
    Purpose
    L-type voltage-gated calcium channel α1C subunit tagged with Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    Cav1.2-Dronpa (CACNA1C Rabbit)
    Use
    Tags
    Full-length rabbit Cav1.2.
    Expression
    Mammalian
    Mutation
    T1066Y, Q1070M
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182333-plasmid-map-sequence-id-363204

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  11. pcDNA3-RIIα-Dronpa

    Plasmid
    #182331
    Purpose
    PKA RIIα subunit-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    RIIalpha-Dronpa (PRKAR2A Human)
    Use
    Tags
    Full-length human PKA type 2alpha regulatory subu…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182331-plasmid-map-sequence-id-363196

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  12. pcDNA3-DrFLINC-AKAR-Kras

    Plasmid
    #182334
    Purpose
    Genetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    DrFLINC-AKAR
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    182334-plasmid-map-sequence-id-363289

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  13. pcDNA3-H2B-μDpTT

    Plasmid
    #182328
    Purpose
    Histone H2B-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    H2B-μDronpa-TagRFP-T (H2BC3 Human)
    Use
    Tags
    Full-length human histone H2B
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    182328-plasmid-map-sequence-id-363396

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  29. pSNRW-Z3-Nluc

    Plasmid
    #174486
    Purpose
    Recombinant fusion protein gene combining IgG-Fc binding domain Z (3 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.
    Depositor
    Richard C. Willson
    Article
    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1
    Insert
    Z3-Nluc
    Use
    Tags
    6x Histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac promoter
    Available since
    Oct. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    174486-plasmid-map-sequence-id-345289

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  31. pAct-GST-H3

    Plasmid
    #182842
    Purpose
    A PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.
    Depositor
    Peter Harte
    Article
    Tie et al Development. 2009 Sep;136(18):3131-41. d
    Insert
    GST-histone H3 fusion
    Use
    Tags
    GST
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    182842-plasmid-map-sequence-id-360059

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  35. pcDNA3-CBX1mut-PA-mCherry

    Plasmid
    #138251
    Purpose
    Triple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.
    Depositor
    Jin Zhang
    Article
    Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:
    Insert
    CBX1mut-PAmCherry
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    138251-plasmid-map-sequence-id-307435

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  39. Q54A

    Plasmid
    #217912
    Purpose
    A plasmid encoding the hydrogel-forming protein PXP with the Q54A coil mutation
    Depositor
    Cole DeForest
    Article
    Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d
    Insert
    PXP Q54A
    Use
    Tags
    6x His (N and C term)
    Expression
    Bacterial
    Mutation
    Glutamine at position 55 and 267 mutated to Alani…
    Promoter
    T5
    Available since
    June 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    217912-plasmid-map-sequence-id-437013

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  40. T40A

    Plasmid
    #217911
    Purpose
    A plasmid encoding the hydrogel-forming protein PXP with the T40A coil mutation
    Depositor
    Cole DeForest
    Article
    Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d
    Insert
    PXP T40A
    Use
    Tags
    6x His (N and C term)
    Expression
    Bacterial
    Mutation
    Threonine at position 41 and 253 mutated to Alani…
    Promoter
    T5
    Available since
    June 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    217911-plasmid-map-sequence-id-437011

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Showing: 281 - 320 of 4777 results