-
Plasmid#178669PurposeBacterial expression of N-terminally 6His tagged A1-LCD with three Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-3R+3K (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationthree Arg residues replaced with LysPromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+7F-7Y
Plasmid#178660PurposeBacterial expression of N-terminally 6His tagged A1-LCD with seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD +7F-7Y (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationseven Tyr residues replaced with PhePromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+12E
Plasmid#178676PurposeBacterial expression of N-terminally 6His tagged A1-LCD with twelve Glu residues added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+12E (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationtwelve Glu residues addedPromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-20G+20S
Plasmid#178683PurposeBacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-20G+20S (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationtwenty Gly residues replaced with SerPromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAD100_hIRG1_4-461_pvp008
Plasmid#124875PurposeExpresses hCAD Arg273His mutant in E.coliDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
UseTagsStrepTag and TEV siteExpressionBacterialMutationmutated Arg273 to His, deleted amino acids 1-3 an…PromoterT7Available sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+7R+12D
Plasmid#178678PurposeBacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+7R+12D (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationseven Arg residues added, twelve Glu residues add…PromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-30G+30S-12F+12Y
Plasmid#178687PurposeBacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-30G+30S-12F+12Y (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationthirty Gly residues replaced with Ser, twelve Phe…PromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-30G+30S+7F-7Y
Plasmid#178686PurposeBacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser, seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-30G+30S+7F-7Y (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationthirty Gly residues replaced with Ser, seven Tyr …PromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-2R-2K+3D
Plasmid#178680PurposeBacterial expression of N-terminally 6His tagged A1-LCD with two Arg residues removed, two Lys removed, three Asp added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-2R+2K+3D (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationtwo Arg residues removed, two Lys added, three As…PromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-20G+20S+7F-7Y
Plasmid#178688PurposeBacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser, seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-20G+20S+7F-7Y (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationtwenty Gly residues replaced with Ser, seven Tyr …PromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+7K+12D
Plasmid#178679PurposeBacterial expression of N-terminally 6His tagged A1-LCD with seven Lys residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+7K+12D (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationseven Lys residues added, twelve Glu residues add…PromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-14N-4Q+18G
Plasmid#178694PurposeBacterial expression of N-terminally 6His tagged A1-LCD with fourteen Gln residues removed, four Asn removed, replaced with eighteen Gly (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-14N-4Q+18G (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationfourteen Gln residues removed, four Asn removed, …PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-4R-2K+5D
Plasmid#178681PurposeBacterial expression of N-terminally 6His tagged A1-LCD with four Arg residues removed, two Lys removed, five Asp added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-4R+2K+5D (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationfour Arg residues removed, two Lys added, five As…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-20G+20S-12F+12Y
Plasmid#178689PurposeBacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-20G+20S-12F+12Y (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationtwenty Gly residues replaced with Ser, twelve Phe…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+23G-23S-12F+12Y
Plasmid#178691PurposeBacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+23G-23S-12F+12Y (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationtwenty-three Ser residues replaced with Gly, twel…PromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+7R+10D
Plasmid#178677PurposeBacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, ten Glu residues added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+7R+10D (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationseven Arg residues added, ten Glu residues addedPromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+23G-23S+7F-7Y
Plasmid#178690PurposeBacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, seven Tyr replaced with Phe (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+23G-23S+7F-7Y (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationtwenty-three Ser residues replaced with Gly, seve…PromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
PCR4-Hsp68::lacZ-H11
Plasmid#139099PurposeenSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterHsp68Available sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
PCR4-Shh::lacZ-H11
Plasmid#139098PurposeenSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterShhAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-R914W_EGFP-PEST_reporter
Plasmid#172596PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-R914W mutant signaling.DepositorInsertmyc-PDGFRA-R914W (PDGFRA Human, Synthetic)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-R914WPromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-D842V_EGFP-PEST_reporter
Plasmid#172598PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V mutant signaling.DepositorInsertmyc-PDGFRA-D842V (PDGFRA Human, Synthetic)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-D842VPromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-D842V+R914W_EGFP-PEST_reporter
Plasmid#172599PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V+R914W mutant signaling.DepositorInsertmyc-PDGFRA-D842V+R914W (PDGFRA Human, Synthetic)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-D842V+R914WPromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-K627R+R914W_EGFP-PEST_reporter
Plasmid#172600PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R+R914W mutant signaling.DepositorInsertmyc-PDGFRA-K627R+R914W (PDGFRA Human, Synthetic)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-K627R+R914WPromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-K627R_EGFP-PEST_reporter
Plasmid#172597PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R mutant signaling.DepositorInsertmyc-PDGFRA-K627R (PDGFRA Human, Synthetic)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-K627RPromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-YFP-KRAS4B
Plasmid#112718PurposeExpresses YFP-KRAS4B fusion protein used for FRET studies in eukaryotic cells.DepositorInsertYFP-KRAS4B (KRAS Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CFP-KRAS4B
Plasmid#112717PurposeExpresses CFP-KRAS4B fusion protein used for FRET studies in mammalian cells.DepositorInsertCFP-KRAS4B (KRAS Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC18-GFP
Plasmid#220495PurposeTo generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity.DepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterSV40Available sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYFP-lacY-.008
Plasmid#140140PurposeEvaluating the impact of feedback and low sensor concentration on information transmission of a genetic sensor.DepositorInsertlacY (lacY )
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYFP-lacY-1
Plasmid#140138PurposeEvaluating the impact of feedback on information transmission of a genetic sensor.DepositorInsertlacY (lacY )
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJH60
Plasmid#172142PurposeMicroscopy inclusion body assayDepositorInsertpCDF.CD
UseTagsExpressionBacterialMutationPylCD partial operon (PylB deleted to assess impa…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYFP-lacY-10
Plasmid#140139PurposeEvaluating the impact of feedback and high sensor concentration on information transmission of a genetic sensor.DepositorInsertlacY (lacY )
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
TAL3463
Plasmid#47182DepositorInsertZebrafishCommunity-Impacthomolog(mouse)-right
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
TAL3462
Plasmid#47181DepositorInsertZebrafishCommunity-Impacthomolog(mouse)-left
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC18-LucFF
Plasmid#220496PurposeTo generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity.DepositorInsertFirefly Luciferase
UseTagsExpressionMammalianMutationPromoterSV40Available sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYFP-x-10
Plasmid#140143PurposeEvaluating the impact of high sensor concentration on information transmission of a genetic sensor.DepositorInsertlacY (lacY )
UseTagsExpressionBacterialMutationD37, I38, and N39 are changed to nonsense (stop) …PromoternoneAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYFP-x-.008
Plasmid#140141PurposeEvaluating the impact of low sensor concentration on information transmission of a genetic sensor.DepositorInsertlacY (lacY )
UseTagsExpressionBacterialMutationD37, I38, and N39 are changed to nonsense (stop) …PromoterAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
GLN, Linear-3, PolyU(1)
Plasmid#166984PurposeTests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.DepositorInsertTermination module:PolyU(1)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27Linear-2, PolyU(0)
Plasmid#166994PurposeTests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination module:PolyU(0)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-1, PolyU(0)
Plasmid#166992PurposeTests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination module:PolyU(0)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
GLY, Linear-3, PolyU(1)
Plasmid#166988PurposeTests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLNYtRNA promoter.DepositorInsertTermination module:PolyU(1)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only