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We narrowed to 62,913 results for: cloning

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Showing: 141 - 160 of 62913 results

  5. lentiSAMv2

    Plasmid
    #75112
    Purpose
    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Publication
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    41 Articles
    75112-plasmid-map-sequence-id-305764

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  8. pTRKH2

    Plasmid
    #71312
    Purpose
    Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.
    Depositor
    Rodolphe Barrangou, Todd Klaenhammer
    Publication
    O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.
    Type
    Empty backbone
    Use
    E. coli-gram positive bacteria shuttle vector
    Expression
    Bacterial
    Available since
    Jan. 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    71312-plasmid-map-sequence-id-323567

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  9. pET28a-LIC

    Plasmid
    #26094
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis and Thrombin cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    9 Articles
    26094-plasmid-map-sequence-id-246717

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  10. pCW-LIC

    Plasmid
    #26098
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    Three consecutive Taq promoters, 2 active
    Expression
    Bacterial
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    12 Articles
    26098-plasmid-map-sequence-id-251071

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  12. pLEX_307

    Plasmid
    #41392
    Purpose
    Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag
    Depositor
    David Root
    Type
    Empty backbone
    Use
    Gateway Cloning and Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    116 Articles
    41392-plasmid-map-sequence-id-331248

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  15. pET28-MHL

    Plasmid
    #26096
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    20 Articles
    26096-plasmid-map-sequence-id-326568

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  16. lentiGuide-Puro

    Plasmid
    #52963
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Publication
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    S. pyogenes sgRNA cassette
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    Ef1-a and hU6
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    873 Articles
    52963-plasmid-map-sequence-id-331247

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  17. pLIX_403

    Plasmid
    #41395
    Purpose
    Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA
    Depositor
    David Root
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector, doxycycli…
    Tags
    V5
    Expression
    Mammalian
    Promoter
    TRE promoter, Tet ON
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74 Articles
    41395-plasmid-map-sequence-id-364439

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  19. pBMN-I-GFP

    Plasmid
    #1736
    Purpose
    This retroviral vector has an IRES-GFP downstream of the cloning sites
    Depositor
    Garry Nolan
    Insert
    IRES-GFP
    Use
    Retroviral
    Available since
    June 20, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    26 Articles
    1736-plasmid-map-sequence-id-418359

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  20. pML104

    Plasmid
    #67638
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation
    Depositor
    John Wyrick
    Publication
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3
    Available since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    29 Articles
    67638-plasmid-map-sequence-id-322882

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Showing: 141 - 160 of 62913 results