165,706 results

1. pJet1.2 kanR cassette

   Plasmid

   #117122

   Purpose

   Kanamycin resistance cassette

   Depositor

   Stephan Gruber

   Article

   Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

   Insert

   Kanamycin resistance cassette for Bacillus subtilis selection
   

   Use

   Golden gate donor vector for gene targeting in ba…

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+cPLA2-mKate2

   Plasmid

   #162068

   Purpose

   Used to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.

   Depositor

   Philipp Niethammer

   Article

   Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

   Insert

   cytosolic phospholipase a2 (pla2g4aa Zebrafish)
   

   Use

   Avian, xenopus, zebrafish

   Tags

   mKate2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKan-AID*-9myc

   Plasmid

   #99522

   Purpose

   C-terminal AID*-9myc degron cassette with KanR marker for S. cerevisiae

   Depositor

   Helle Ulrich

   Article

   Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

   Insert

   IAA17(71-116) (AXR3 Mustard Weed)
   

   Use

   Pcr template for yeast tagging cassette

   Tags

   9xMyc

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-FOXG1

   Plasmid

   #153124

   Purpose

   Expression of Flag tagged human FOXG1 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXG1 (FOXG1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV2-SEP-GluA1 (M1)

   Plasmid

   #64942

   Purpose

   mammalian expression of rat GluA1

   Depositor

   Jeremy Henley

   Article

   Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

   Insert

   GluA1 (Gria1 Rat)
   

   Tags

   SEP (superecliptic pHluorin)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-CA sfGFP

   Plasmid

   #159094

   Purpose

   PB-CA sfGFP piggyBac transposon

   Depositor

   Mario Capecchi

   Insert

   sfGFP
   

   Use

   Piggybac

   Expression

   Mammalian

   Available Since

   Nov. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTDH3-dCas9

   Plasmid

   #46920

   Purpose

   Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoter

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   TDH3

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGM6

   Plasmid

   #110660

   Purpose

   Adeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo transduction of muscle, lung, liver and other tissues.

   Depositor

   David Russell

   Article

   Gregorevic et al Nat Med. 2004 Aug;10(8):828-34. doi: 10.

   Insert

   AAV6 cap genes, AAV2 rep genes, adenovirus helper genes
   

   Use

   AAV

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tol2-CAG::Mitbow

   Plasmid

   #158994

   Purpose

   Multicolor mitochondrial-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing mito-tethered mEYFP, mCherry or mCerulean upon Cre recombination)

   Depositor

   Jean Livet

   Article

   Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

   Insert

   Mitbow
   

   Tags

   The default expressed EBFP2 is fused to H2B; oth…

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-AID-Ns3h-UGI-T2A-mTagBFP2

    Plasmid

    #221223

    Purpose

    Express HACE Editor as a fusion of AID cytidine deaminase and Ns3h helicase with mTagBFP2 reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-Ns3h-UGI-T2A-mTagBFP2
    

    Expression

    Mammalian

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-TetON-3G

    Plasmid

    #96963

    Purpose

    Mammalian expression of Clontech rtTA driven by human beta-actin promoter

    Depositor

    Elena Cattaneo

    Article

    Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOG44_FLP_recombinase (pEHA1338)

    Plasmid

    #209087

    Purpose

    FLP recombinase for FRT-mediated integration

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Insert

    FLP recombinase
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMOS011: superecliptic pHluorin (mitochondrial)

    Plasmid

    #163050

    Purpose

    superecliptic pHluorin (mitochondrial)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    superecliptic pHluorin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLIK CA ROCK2

    Plasmid

    #84649

    Purpose

    Lentiviral expression of doxycycline-inducible constitutively active ROCK2 and co-expression of Venus

    Depositor

    Sanjay Kumar

    Article

    Wong et al Cancer Res. 2015 Mar 15;75(6):1113-22. d

    Insert

    ROCK2 (ROCK2 Bovine)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Constitutive Active (please see depositor comment…

    Available Since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya

    Plasmid

    #186980

    Purpose

    mammalian expression of Cluc-Gluc reporter

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

    Insert

    Cluc, Gluc
    

    Use

    Luciferase

    Available Since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEIGW-human KLF4

    Plasmid

    #100131

    Purpose

    Human KLF4 lentiviral expression vector

    Depositor

    Eva Hernando

    Article

    Segura et al Oncotarget. 2017 Apr 18;8(16):26298-2631

    Insert

    Kruppel like factor 4 (KLF4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    E1F

    Available Since

    Feb. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF0174

    Plasmid

    #141541

    Purpose

    Lentiviral vector for overexpressing the RELA transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RELA (RELA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHH0103_YWHAG_14-3-3

    Plasmid

    #109905

    Purpose

    Protein expression and purification of YWHAG_14-3-3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    YWHAG_14-3-3 (YWHAG Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAcBac1-Mb-sfGFP 150TAG

    Plasmid

    #197570

    Purpose

    Expression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP 150TAG
    Pyl-tRNA (4 copies)
    

    Tags

    V5-His6

    Expression

    Mammalian

    Mutation

    N150TAG

    Promoter

    CMV and U6/H1

    Available Since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSEMS-CV SUy-Halo7Tag

    Plasmid

    #108933

    Purpose

    Selflabeling tag at ATP Synthase

    Depositor

    Karin Busch

    Article

    Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

    Insert

    ATP synthase F1 subunit gamma (ATP5F1C Human)
    

    Tags

    Halo7 tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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