We narrowed to 7,668 results for: CCH

1. ClhN-P1K-3VSV-Atg8(L55W T56A V61W R65A)-MET15

   Plasmid

   #207054

   Purpose

   Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Tags

   3xVSV

   Expression

   Yeast

   Mutation

   L55W T56A V61W R65A

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEE059

   Plasmid

   #176816

   Purpose

   Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

   Insert

   610
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKdH_P-GAP-ScACS1*

   Plasmid

   #126736

   Purpose

   yeast plasmid overexpressing ACS1 from Saccharomyces cerevisiae

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   acs1*
   

   Tags

   6xHis,Myc

   Expression

   Yeast

   Mutation

   T3542C and A30S, P113S, and S629N (please see dep…

   Promoter

   GAP

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKdH_P-GAP-ScADH2+P-GAP*-ScACS1*

   Plasmid

   #126738

   Purpose

   yeast plasmid overexpressing ADH2 and ACS1 from Saccharomyces cerevisiae

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   adh2,acs1*
   

   Tags

   6xHis,Myc

   Expression

   Yeast

   Mutation

   T3542C

   Promoter

   GAP

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-EGFP-NLS-P2A-mCherry-PTS1

   Plasmid

   #87803

   Purpose

   Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.

   Depositor

   Andrea Musacchio

   Article

   Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

   Inserts

   NLS
   PTS1
   

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Promoter

   CMV promoter, tetracycline operator

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. mClY-N1

   Plasmid

   #90457

   Purpose

   A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

   Depositor

   Joseph Santos-Sacchi

   Article

   Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

   Insert

   mClY-N1
   

   Expression

   Mammalian

   Mutation

   EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCLXEBR-pTF-cKit

   Plasmid

   #114293

   Purpose

   TET-inducible expression of c-Kit

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Insert

   cKit (Kit Mouse)
   

   Use

   Lentiviral

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1

   Plasmid

   #87828

   Purpose

   High-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.

   Depositor

   Andrea Musacchio

   Article

   Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

   Inserts

   NLS
   PTS1
   

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Promoter

   CMV promoter, tetracycline operator

   Available Since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLIB-HIS-TEV-CKS1

   Plasmid

   #177013

   Purpose

   Generate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEV

   Depositor

   Andrea Musacchio

   Article

   Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

   Insert

   CKS1 (CKS1B Human, Synthetic)
   

   Tags

   Polyhistidine-TEV

   Expression

   Insect

   Mutation

   codon-optimised for insect cell expression

   Available Since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6xHis-TEV-ATG3 (Human autophagy E2-like enzyme)

    Plasmid

    #169079

    Purpose

    Human ATG3

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    ATG3 (ATG3 Human)
    

    Tags

    6X Histidine Tag, TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 lac promoter

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSUMO-β′-COPI-WD40

    Plasmid

    #180753

    Purpose

    Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40 (residues 1-301).

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    β′-COPI-WD40 (SEC27 Budding Yeast)
    

    Tags

    N-terminal strep tag, His-tag, and SUMO tag

    Expression

    Bacterial

    Mutation

    Encodes residues 1-301; deleted 302-889; mutated …

    Promoter

    T7

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti-HA-mBec1

    Plasmid

    #99505

    Purpose

    3rd generation lentiviral expression of wild-type Becn1

    Depositor

    Congcong He

    Article

    Rocchi et al PLoS Genet. 2017 Aug 14;13(8):e1006962.

    Insert

    Beclin 1 (Becn1 Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    CMV

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

    Plasmid

    #87827

    Purpose

    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    NLS
    PTS1
    

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Promoter

    CMV promoter, tetracycline operator

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCCLc-MNDU3-LIN28A-PGK-EGFP-WPRE

    Plasmid

    #89607

    Purpose

    Expression of LIN28A in human cells

    Depositor

    Fernando Fierro

    Article

    Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

    Insert

    LIN28A (LIN28A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    MNDU3

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. G31, Cdc20-AID tagging

    Plasmid

    #130269

    Purpose

    Tagging the Saccharomyces cerevisiae Cdc20 gene with a auxin-degron tag, via homologous recombination

    Depositor

    Matthias Heinemann

    Article

    Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

    Insert

    Cdc20/AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)
    

    Tags

    AID(71-114)

    Expression

    Yeast

    Promoter

    Native promoter

    Available Since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCWXPGR-pTF-DVL2deltaDIX

    Plasmid

    #114276

    Purpose

    TET-inducible expression of DVL2 protein with a deletion of its DIX-domain

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    DVL2 (Dvl2 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    deletion of DIX domain

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1*

    Plasmid

    #126739

    Purpose

    yeast plasmid overexpressing ADH2,ALD6 and ACS1 from Saccharomyces cerevisiae

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    adh2, acs1*, ald6
    

    Tags

    6xHis,Myc

    Expression

    Yeast

    Mutation

    T3542C and A30S, S629N (please see depositor comm…

    Promoter

    GAP

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. SSH-pSUMO-β′-COPI-WD40-Tyr33Ala

    Plasmid

    #208170

    Purpose

    Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Tyr33Ala mutant (residues 1-301)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    N-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Tyr33Ala substitution
    

    Tags

    His-tag, SUMO-tag, and Strep-tag

    Expression

    Bacterial

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-α-COPI-WD40-Arg57Ala

    Plasmid

    #180759

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg57Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40-Arg57Ala
    

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Arg57Ala, Leu1…

    Promoter

    CMV

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-α-COPI-WD40-Tyr139Ala

    Plasmid

    #180761

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Tyr139Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40-Tyr139Ala
    

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Tyr139Ala, Leu…

    Promoter

    CMV

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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