We narrowed to 2,542 results for: pcas9

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p458 VQR

Plasmid

#101727

Purpose

Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VQR

Expression

Mammalian

Mutation

VQR

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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KA2058_pX330A-G2P

Plasmid

#124202

Purpose

Expression of SpCas9 and sgRNA

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pIVT-Cas9-DEN2

Plasmid

#238017

Purpose

for DNA-free gene knockout editing in rice and wheat or other plants

Depositor

Caixia Gao

Article

Qiu et al Plant Biotechnol J. 2025 Apr;23(4):1348-

Insert

SpCas9

Expression

Plant

Promoter

T7

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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piCRg Entry

Plasmid

#58904

Purpose

Cas9/gRNA Entry expression plasmid

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Expression

Mammalian

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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px458_Conc2

Plasmid

#134449

Purpose

Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFP

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pICSL1P1_AtU626Ter26

Plasmid

#191769

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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p206_LTJ_sgRNACD45.2_R3

Plasmid

#82674

Purpose

sgRNA targeting murine CD45.2 region 3. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting CD45.2 region 3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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px458_Conc3

Plasmid

#134450

Purpose

Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + GFP

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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ABE-RA2.0

Plasmid

#208299

Purpose

Expresses ABE-RA2.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA2.0-SpCas9 D10A

Expression

Mammalian

Mutation

P48A, R51H, I76F, D108G, K110R, H122R, M126I, N12…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA3.1

Plasmid

#208302

Purpose

Expresses ABE-RA3.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.1-SpCas9 D10A

Expression

Mammalian

Mutation

E27D, R47K, P48A, R51H, I76F, D108G, K110R, H122R…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMH220

Plasmid

#122836

Purpose

dCas9 with GreenGate B-C flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis

Use

Golden gate compatible cloning vector

Mutation

Contains D10A and H840A mutations

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSCL.140

Plasmid

#184995

Purpose

Express Cas9 in human cells

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

SpCas9

Tags

SV40NLS

Expression

Mammalian

Promoter

TetOn-3g

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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p184_LTJ_sgRNACD90.2

Plasmid

#82670

Purpose

sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting mouse CD90.2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pOC4 cloning template vector

Plasmid

#183423

Purpose

FlpON knock-in vector with FLAG-SpCas9 expression

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDU1

Plasmid

#169817

Purpose

pSpCas9(BB)-2A-tCD19 (pDU1)

Depositor

Andrea Rossi

Article

Ramachandran et al Life Sci Alliance. 2021 Apr 26;4(6). pii

Insert

tCD19

Tags

fusion protein

Expression

Mammalian

Promoter

Cbh

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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USP19 sgRNA1

Plasmid

#78585

Purpose

delete USP19 gene in human cell

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

USP19 sgRNA1

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
py100-sgRNA_hVEGFA

Plasmid

#198282

Purpose

Expression of SpCas9 guide RNA specific for human VEGFA locus

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

py100-sgRNA_hVEGFA

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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px330-USP13

Plasmid

#61812

Purpose

to make USP13 Knockout cell line

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13 (USP13 Human)

Use

CRISPR

Promoter

U6

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only
Yeast_Target-AID3S

Plasmid

#188646

Purpose

Expresses Target-AID3S in yeasts

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nCas9-AID2S-UGI

Tags

UGI

Expression

Yeast

Mutation

D10A for SpCas9

Promoter

pGal1

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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epiCRISPRn

Plasmid

#135961

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

Type

Empty backbone

Expression

Mammalian

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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