We narrowed to 12,292 results for: BASE

1. pFA6a-GFP(S65T)-TRP1

   Plasmid

   #41597

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   TRP1 (TRP1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   GFP (S65T) and yeast ADH1 terminator

   Expression

   Mutation

   Promoter

   Available since

   Jan. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. P16ΔS:S10-(MCS)

   Plasmid

   #108182

   Purpose

   Binary vectors used for the constitutive expression of tripartite split-sfGFP components in plants

   Depositor

   Tzu-Yin Liu

   Article

   Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   P16ΔS

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMCP-RecT

   Plasmid

   #183827

   Purpose

   pLenti-EF1A-MCP-EXTEN-RecT-NLS

   Depositor

   Le Cong

   Article

   Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

   Insert

   MCP-RecT_NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. qTAG-KO-Puro

   Plasmid

   #227262

   Purpose

   Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMK445 (BSD-P2A-BromoTag)

   Plasmid

   #214375

   Purpose

   BromoTag for N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   BSD-P2A-BromoTag
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. qTAG-N-Blast-mStayGold

   Plasmid

   #227251

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mStayGold cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pT7-VP2SA11

   Plasmid

   #89163

   Purpose

   Express Rotavirus SA11 VP2 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 VP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promotor

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT7-VP1SA11

   Plasmid

   #89162

   Purpose

   Express Rotavirus SA11 VP1 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 VP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promotor

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. qTAG-N-Puro-ultraID

   Plasmid

   #207693

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-ultraID cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pT7-NSP4SA11

    Plasmid

    #89171

    Purpose

    Express Rotavirus SA11 NSP4 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 NSP4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSEVA221pmCDA1-T7RNAP

    Plasmid

    #167978

    Purpose

    Expresses fusion protein pmCDA1-T7RNAP in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

    Insert

    pmCDA1-T7RNAP (T7p07 Synthetic)
    

    Use

    Tags

    T7 tag and T7RNAP

    Expression

    Bacterial

    Mutation

    Promoter

    TetR-Ptet

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT7-VP3SA11

    Plasmid

    #89164

    Purpose

    Express Rotavirus SA11 VP3 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT7-VP4SA11

    Plasmid

    #89165

    Purpose

    Express Rotavirus SA11 VP4 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT7-NSP3SA11

    Plasmid

    #89170

    Purpose

    Express Rotavirus SA11 NSP3 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 NSP3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. qTAG-N-Puro-mScarlet

    Plasmid

    #207689

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mScarlet cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. qTAG-C-3xFLAG-Blast

    Plasmid

    #207717

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pT7-NSP1SA11

    Plasmid

    #89168

    Purpose

    Express Rotavirus SA11 NSP1 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 NSP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. qTAG-N-Puro-3xFLAG

    Plasmid

    #207695

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xFLAG cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. qTAG-C-mNeon-Puro

    Plasmid

    #207720

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. qTAG-N-Puro-mNeon

    Plasmid

    #207687

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mNeon cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRDA_429

    Plasmid

    #179098

    Purpose

    BE Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    ABE8e (Cas9-NG)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p663-UBC-TurboID-V5-CDK13_IDG-K

    Plasmid

    #135267

    Purpose

    Gateway destination clone of CDK13 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    TurboID-V5-CDK13 (CDK13 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pT7-NSP5SA11

    Plasmid

    #89172

    Purpose

    Express Rotavirus SA11 NSP5 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 NSP5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pT7-NSP2SA11

    Plasmid

    #89169

    Purpose

    Express Rotavirus SA11 NSP2 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 NSP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLR-NI

    Plasmid

    #31006

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    last NI repeat
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLR-NG

    Plasmid

    #30995

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    last NG repeat
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLR-HD

    Plasmid

    #30984

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    last HD repeat
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLR-NN

    Plasmid

    #31017

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    last NN repeat
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. qTAG-C-3xFLAG-Puro

    Plasmid

    #207728

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pT7-VP6SA11

    Plasmid

    #89166

    Purpose

    Express Rotavirus SA11 VP6 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pT7-VP7SA11

    Plasmid

    #89167

    Purpose

    Express Rotavirus SA11 VP7 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP7
    

    Use

    Tags

    *see below

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Level 1 P4 TaU6 guide acceptor

    Plasmid

    #165600

    Purpose

    GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid

    Depositor

    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    TaU6 promoter::LacZ::sgRNA scaffold
    

    Use

    CRISPR and Synthetic Biology; Sgrna acceptor with…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Level 1 P3 TaU6 guide acceptor

    Plasmid

    #165599

    Purpose

    GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid

    Depositor

    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    TaU6 promoter::LacZ::sgRNA scaffold
    

    Use

    CRISPR and Synthetic Biology; Sgrna acceptor with…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLV hUbC-dCas9-T2A-GFP

    Plasmid

    #53191

    Purpose

    Co-expresses human optimized S. pyogenes dCas9 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBJ2

    Plasmid

    #167131

    Purpose

    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Kanamycin promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCT-tRNA

    Plasmid

    #133813

    Purpose

    CRISPR-Cas9 system for genetic manipulation of Candida tropicalis

    Depositor

    Geraldine Butler

    Article

    Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

    Insert

    cassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin

    Plasmid

    #134662

    Purpose

    Expresses EGFP-tagged plasma membrane marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    vesicle-associated membrane protein 5 (VAMP5 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFA6a-His3MX6

    Plasmid

    #41596

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    His3
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Dimer cleaved

    Plasmid

    #198199

    Purpose

    FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavage

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    mVenus-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K both

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TALED_Left-ND1-AD-E1347A

    Plasmid

    #183894

    Purpose

    Expresses TALED_Left-ND1-AD-E1347A in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    TALED for ND1 mutation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge